Anti-ABAT/GABA-T 抗体 (ab152134)
Key features and details
- Rabbit polyclonal to ABAT/GABA-T
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-ABAT/GABA-T antibody
ABAT/GABA-T 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ABAT/GABA-T -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment within Human ABAT/GABA-T. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Centre region
Database link: P80404 -
ポジティブ･コントロール
- SK-N-SH, Mouse brain and Rat brain whole cell lysates; HepG2 cells; U373 xenograft.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab152134の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
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| IHC-P |
1/100 - 1/1000.
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| ICC/IF |
1/100 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 56 kDa. |
|
IHC-P
1/100 - 1/1000. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine. -
組織特異性
Liver > pancreas > brain > kidney > heart > placenta. -
関連疾患
Defects in ABAT are a cause of gamma-aminobutyrate transaminase deficiency (GABA-AT deficiency) [MIM:613163]. The phenotype of this deficiency includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. -
配列類似性
Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 18 Human
- Entrez Gene: 268860 Mouse
- Entrez Gene: 81632 Rat
- Omim: 137150 Human
- SwissProt: P80404 Human
- SwissProt: P61922 Mouse
- SwissProt: P50554 Rat
- Unigene: 336768 Human
see all -
別名
- (S) 3 amino 2 methylpropionate transaminase antibody
- (S)-3-amino-2-methylpropionate transaminase antibody
- 4 aminobutyrate aminotransferase antibody
see all
画像
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Western blot - Anti-ABAT/GABA-T antibody (ab152134)Anti-ABAT/GABA-T antibody (ab152134) at 1/5000 dilution + Mouse brain whole cell lysate at 20 µg
Predicted band size: 56 kDa
7.5% SDS PAGE -
Western blot - Anti-ABAT/GABA-T antibody (ab152134)Anti-ABAT/GABA-T antibody (ab152134) at 1/1000 dilution + Rat brain whole cell lysate at 50 µg
Predicted band size: 56 kDa
7.5% SDS PAGE -
Western blot - Anti-ABAT/GABA-T antibody (ab152134)Anti-ABAT/GABA-T antibody (ab152134) at 1/1000 dilution + SK-N-SH whole cell lysate at 30 µg
Predicted band size: 56 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-ABAT/GABA-T antibody (ab152134)Immunofluorescent analysis of methanol-fixed HepG2 cells labeling ABAT/GABA-T with ab152134 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABAT/GABA-T antibody (ab152134)Immunohistochemical analysis of paraffin-embedded U373 xenograft labeling ABAT/GABA-T with ab152134 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab152134 は 1 報の論文で使用されています。
- Huo Q et al. Prefrontal Cortical GABAergic Dysfunction Contributes to Aberrant UP-State Duration in APP Knockout Mice. Cereb Cortex : (2016). PubMed: 27552836