JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB291243

Anti-AAV5 抗体 [HL2476] - BSA and Azide free

Anti-AAV5 antibody [HL2476] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse Recombinant Monoclonal AAV5 antibody. Carrier free. Suitable for I-ELISA, Dot and reacts with Recombinant fragment - adeno-associated virus 5 samples.
2 Images
Indirect ELISA - Anti-AAV5 antibody [HL2476] - BSA and Azide free (AB291243)
  • I-ELISA

Lab

Indirect ELISA - Anti-AAV5 antibody [HL2476] - BSA and Azide free (AB291243)

This data was developed using ab315831, the same antibody clone in a different buffer formulation.

Indirect ELISA showing ab315829 binding to AAV8 & AAV3B empty capsid. Binding of ab315829 was assessed in a serial dilution and EC-50 is 1.234 ng/ml for AAV8 and 6.202 ng/ml for AAV3B. The secondary antibody used was Goat Anti-Mouse IgG H&L (HRP) preadsorbed at 1/2000 dilution.

Antigen : AAV1 empty capsid,AAV2 empty capsid,AAV5 empty capsid,AAV6 empty capsid,AAV8 empty capsid,AAV9 empty capsid AAV3B empty capsid.

Antigen concentration : 7.5E+10 vp/mL

Dot Blot - Anti-AAV5 antibody [HL2476] - BSA and Azide free (AB291243)
  • Dot

Supplier Data

Dot Blot - Anti-AAV5 antibody [HL2476] - BSA and Azide free (AB291243)

This data was developed using ab315831, the same antibody clone in a different buffer formulation.

Dot blot analysis of AAV5 using ab315831 at 1 : 2000 (0.5 ug/ml) followed by a Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1 : 5000 dilution.

Dot blot was performed using 1 ng of each capsid loaded per well.

Anti-AAV9 antibody [HL2368] (ab315818) (1 : 2000), Anti-AAV9 antibody [HL2370] (ab315820) (1 : 2000), Anti-AAV9 antibody [HL2374] (ab315823) (1 : 2000), and Anti-AAV9 antibody [HL2374-IgG1] - BSA and Azide free (ab315826) (1 : 2000) specifically recognize AAV9 capsid.

Anti-AAV8 + AAV3B antibody [HL2383] (ab315827) (1 : 2000), and Anti-AAV8 + AAV3B antibody [HL2383-IgG1] (ab315829) (1 : 2000) specifically recognize AAV8/3B capsid.

Anti-AAV8 + AAV9 antibody [HL2372] (ab315821) (1 : 2000) specifically recognizes AAV8/9 capsid.

Anti-AAV5 antibody [HL2476] (ab315831) (1 : 2000) specifically recognizes AAV5 capsid.

false

Exposure time: 8min

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

HL2476

アイソタイプ

IgG2b

キャリアフリー

Yes

交差種

adeno-associated virus 5

アプリケーション

I-ELISA, Dot

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - adeno-associated virus 5": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>" }, "adeno-associated virus 5": { "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "" } } }
style
left-column

製品の詳細

ab291243 is the carrirer-free version of ab315831.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The biological target AAV5 or Adeno-Associated Virus type 5 is a non-pathogenic virus used widely as a vector in gene therapy. This virus has a protein structure named the AAV5 capsid which serves as its protective shell. The AAV5 capsid has an estimated mass of 60 megadaltons and plays an important role in virus stability and infectivity. AAV5 is expressed in various tissues but its expression pattern can differ based on the type of tissue and species being studied.
Biological function summary

The AAV5 capsid facilitates entry into host cells by interacting with cellular receptors. It is not a component of a larger complex but its role is important for delivering genetic material into cells. The virus's ability to transduce a wide range of cell types makes it highly effective in gene therapy applications. This versatility allows researchers to target specific tissues and diseases.

Pathways

AAV5 plays an important role in gene delivery systems which can impact the expression of genetically targeted therapies. Its interaction with heparan sulfate proteoglycans during cellular entry highlights its involvement in pathways related to cellular uptake and transfer of genetic material. This function is highly relevant when considering AAV5 as a tool for introducing therapeutic genes into cells alongside proteins like other adeno-associated viruses which may share similar pathways.

AAV5 has emerged as a powerful tool in the treatment of genetic disorders such as hemophilia and neurodegenerative diseases. Its ability to transduce non-dividing cells makes it suitable for long-term expression in cells. AAV5's interaction with the coagulation factor IX protein can provide therapeutic benefits in hemophilia B patients when delivered effectively. It also shows promise in delivering genes to neural tissues potentially aiding in treating disorders like Parkinson’s disease.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

See full target information AAV5
style
left-column
style
left-column
style
right-column

組合せ製品

Primary Antibodies

AB2722

Anti-NFAT1 antibody [25A10.D6.D2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT1 antibody [25A10.D6.D2] (AB2722)

  • 5
  • 7
Primary Antibodies

AB9879

Anti-Mumps nucleoprotein antibody [5E3]

Western blot - Anti-Mumps nucleoprotein antibody [5E3] (AB9879)

  • 0
  • 0
Primary Antibodies

AB109864

Anti-Adenine Nucleotide Translocase Immunocapture antibody [5F51BB5AG7]

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenine Nucleotide Translocase Immunocapture antibody [5F51BB5AG7] (AB109864)

  • 0
  • 0
Primary Antibodies

AB2596

Anti-Adenovirus type 1 hexon E11 antibody [1-M-13]

  • 0
  • 0

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com