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リコンビナントRabMAb

Anti-AACT 抗体 [EPR14117(B)] (ab180492)

Submit a review Submit a question References (1)
Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)
  • Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)
  • Immunoprecipitation - Anti-AACT antibody [EPR14117(B)] (ab180492)
  • Anti-AACT antibody [EPR14117(B)] (ab180492)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14117(B)] to AACT
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-AACT antibody [EPR14117(B)]
    AACT 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14117(B)] to AACT

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WB, IPmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human plasma and colon cancer lysates; Human liver and placenta tissues.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab180492の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 47 kDa).
IP
1/70.
特記事項
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 47 kDa).
IP
1/70.

ターゲット情報

  • 機能

    Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.

  • 組織特異性

    Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.

  • 関連疾患

    Defects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280].

  • 配列類似性

    Belongs to the serpin family.

  • ドメイン

    The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • SERPINA3 antibody
      • AACT antibody
      • AACT_HUMAN antibody
      • ACT antibody
      • alpha 1 Antichymotrypsin antibody
      • Alpha-1-antichymotrypsin His-Pro-less antibody
      • Antichymotrypsin antibody
      • Cell growth inhibiting gene 24/25 protein antibody
      • Cell growth-inhibiting gene 24/25 protein antibody
      • GIG24 antibody
      • GIG25 antibody
      • Growth inhibiting protein 24 antibody
      • Growth inhibiting protein 25 antibody
      • MGC88254 antibody
      • Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 3 antibody
      • Serine (or cysteine) proteinase inhibitor clade A member 3 antibody
      • Serine proteinase inhibitor clade A member 3 antibody
      • Serpin A3 antibody
      • Serpin family A member 3 antibody
      • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3 antibody
      • SERPINA3 antibody
      see all

    画像

    • Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Anti-AACT antibody [EPR14117(B)] (ab180492) at 1/10000 dilution + Human plasma lysate at 20 µg

      Secondary
      Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size: 47 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Western blot - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Anti-AACT antibody [EPR14117(B)] (ab180492) at 1/2000 dilution + Human colon cancer tissue lysate at 20 µg

      Secondary
      Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size: 47 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling AACT with ab180492 at 1/500 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody [EPR14117(B)] (ab180492)

      Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling AACT with ab180492 at 1/500 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-AACT antibody [EPR14117(B)] (ab180492)
      Immunoprecipitation - Anti-AACT antibody [EPR14117(B)] (ab180492)

      Western blot analysis of  Human plasma immunoprecipitated using ab180492 at 1/70 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate was used as secondary antibody, at 1/1000 dilution.

    • Anti-AACT antibody [EPR14117(B)] (ab180492)
      Anti-AACT antibody [EPR14117(B)] (ab180492)

    参考文献 (1)

    ab180492 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab180492 は 1 報の論文で使用されています。

    • Li Y  et al. SERPINA3 induced by astroglia/microglia co-culture facilitates glioblastoma stem-like cell invasion. Oncol Lett 15:285-291 (2018). PubMed: 29399139

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