Anti-AACT 抗体 (ab9374)
Key features and details
- Rabbit polyclonal to AACT
- Suitable for: IHC-P, Sandwich ELISA, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-AACT antibody
AACT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to AACT -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, Sandwich ELISA, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mammals
非交差種:
Mouse -
免疫原
Full length native protein (purified) corresponding to AACT. Alpha-1-antichymotrypsin isolated from human serum.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.3
Constituents: PBS, 0.01425% Magnesium chloride, 0.00154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.01% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9374の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/25 - 1/50. ABC method.
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| Sandwich ELISA |
Use at an assay dependent concentration.
For sandwich ELISA, use this antibody as Detection at an assay dependent concentration with Mouse monoclonal to AACT (ab54693) as Capture. |
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 48 kDa).
Assay Dependent |
| 特記事項 |
|---|
|
IHC-P
1/25 - 1/50. ABC method. |
|
Sandwich ELISA
Use at an assay dependent concentration. For sandwich ELISA, use this antibody as Detection at an assay dependent concentration with Mouse monoclonal to AACT (ab54693) as Capture. |
|
WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 48 kDa). Assay Dependent |
ターゲット情報
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機能
Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. -
組織特異性
Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains. -
関連疾患
Defects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280]. -
配列類似性
Belongs to the serpin family. -
ドメイン
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 12 Human
- Omim: 107280 Human
- SwissProt: P01011 Human
- Unigene: 534293 Human
- Unigene: 710488 Human
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別名
- SERPINA3 antibody
- AACT antibody
- AACT_HUMAN antibody
see all
画像
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Western blot - Anti-AACT antibody (ab9374)All lanes : Anti-AACT antibody (ab9374) at 1 µg/ml
Lane 1 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 2 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 48 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
Sandwich ELISA - Anti-AACT antibody (ab9374)Standard Curve for AACT (Analyte: AACT protein (Human) (ab80517) ); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to AACT (ab54693) at 5µg/ml and Detector Antibody Rabbit polyclonal to AACT (ab9374) at 0.5µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AACT antibody (ab9374)Human normal skin. Staining is mainly membrane-bound and cytoplasmic. Left panel: with primary antibody at 2 ug/ml. Right panel: negative control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for rabbit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Western blot - Anti-AACT antibody (ab9374)
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab9374 は 1 報の論文で使用されています。
- Martin-Rojas T et al. A clinical perspective on the utility of alpha 1 antichymotrypsin for the early diagnosis of calcific aortic stenosis. Clin Proteomics 14:12 (2017). WB, IHC . PubMed: 28439213
