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Anti-8-Hydroxy-2'-deoxyguanosine 抗体 [N45.1] (ab48508)

Reviews (9)Q&A (12)References (145)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)

Key features and details

  • Mouse monoclonal [N45.1] to 8-Hydroxy-2'-deoxyguanosine
  • Suitable for: IHC-P
  • Reacts with: Species independent
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1]

  • 製品の詳細

    Mouse monoclonal [N45.1] to 8-Hydroxy-2'-deoxyguanosine

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-Pmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Chemical/ Small Molecule: 8-Hydroxy-2'-deoxyguanosine conjugated Keyhole Limpet Hemocyanin

  • ポジティブ･コントロール

    • Epidermis from hairless mouse treated for 4 weeks with chronic UVB irradiation, or kidney treated with Fe-NTA.

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 27th February 2018.  Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab48508の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (3)
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

For mouse on mouse staining we recommend uisng MOM kit - ab127055.
特記事項
IHC-P
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

For mouse on mouse staining we recommend uisng MOM kit - ab127055.

ターゲット情報

  • 関連性

    8-hydroxy-2'-deoxyguanosine is a biomarker of oxidative DNA damage.

  • 別名

    • 8 OHdG antibody
    • 8-OHdG antibody
    • 8OHdG antibody

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)Image from Wu L et al., PLoS ONE 8(4): e60262. Fig 6 doi:10.1371/journal.pone.0060262 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    De-paraffinized and dehydrated sections were treated in a microwave oven at low power for 10 min in 0.01 M sodium citrate buffer (pH 6.0) to retrieve antigen, and inactivated endogenous peroxidase using 3% hydrogen peroxide in methanol for 10 min, and blocked with blocking solution for 1 hour at room temperature. Sections were then incubated with 1∶20 dilution of mouse monoclonal antibody to 8-Hydroxy-2'-deoxyguanosine (ab48508) overnight at 4°C. For detection, a biotinylated anti-mouse and HRP-labeled Streptavidin, and staining with DAB was used.

    Immunohistochemical staining of nitrotyrosine (A) and 8-hydroxy-2′-deoxyguanosine (B) was performed on 5 rats randomly chosen from each group (ND: chow diet group, HFD: High fat diet, HFD-QH: High fat diet treated with Qing Huo Yi Hao" (QHYH)). An islet is encircled in each quandrant. Black arrows point to islets of Langerhans (Original magnification ×200). Percentage of immunohistochemial positive staining area in islet out of the area of corresponding islet (C, D) was presented. Values and bars represent means±SEM. * and ** denote P<0.05 and P<0.001 versus group ND.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)This image is courtesy of an Abreview submitted by Rudolf Jung

    ab48508 staining 8-Hydroxy-2'-deoxyguanosine in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a TE buffer. Samples were incubated with primary antibody (1/50) for 30 mins at 20°C. A HRP-conjugated Goat anti-mouse IgG was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (ab48508)
    Ab48508, at a concentration of 5 µg/ml, staining 8-Hydroxy-2'-deoxyguanosine in epidermis from hairless mouse after 4 weeks of treatment by chronic UVB irradiation.

参考文献 (145)

ab48508 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab48508 は 145 報の論文で使用されています。

  • Fang Z  et al. Oxidative stress-triggered Wnt signaling perturbation characterizes the tipping point of lung adeno-to-squamous transdifferentiation. Signal Transduct Target Ther 8:16 (2023). PubMed: 36627278
  • Tanushi X  et al. OGG1 competitive inhibitors show important off-target effects by directly inhibiting efflux pumps and disturbing mitotic progression. Front Cell Dev Biol 11:1124960 (2023). PubMed: 36819096
  • Ragu S  et al. A noncanonical response to replication stress protects genome stability through ROS production, in an adaptive manner. Cell Death Differ 30:1349-1365 (2023). PubMed: 36869180
  • Huang A  et al. Bardoxolone Methyl Ameliorates Myocardial Ischemia/Reperfusion Injury by Activating the Nrf2/HO-1 Signaling Pathway. Cardiovasc Ther 2023:5693732 (2023). PubMed: 36874247
  • Park J  et al. Combined Effects of Focused Ultrasound and Photodynamic Treatment for Malignant Brain Tumors Using C6 Glioma Rat Model. Yonsei Med J 64:233-242 (2023). PubMed: 36996894
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-10 of 21 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MC3T3 cells)
Permeabilization
Yes - NP40
Specification
MC3T3 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 07 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - NP40
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 06 2020

Western blot abreview for Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - nuclear (MC3T3 cells)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (No gel used)
Loading amount
1000 cells
Treatment
20 uM or 100 uM H2O2 for 12 hours
Specification
MC3T3 cells
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 03 2020

Western blot abreview for Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - nuclear (Vascular smooth muscle cells)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (NO gel used)
Loading amount
1000 cells
Treatment
20 uM or 100 uM H2O2 for 12 hours
Specification
Vascular smooth muscle cells
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 03 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: PH=6 ,citrate buffer
Permeabilization
No
Specification
skin
Blocking step
Rodent Block from ab127055 as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
10% Formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 10 2018

Immunohistochemistry (Frozen sections) abreview for Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver)
Permeabilization
Yes - 0.3%triton-X
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Yoshihisa Koyama

Verified customer

投稿 Dec 22 2017

Question


I’ve just received the Ab ab48508 I’ve ordered, thank you. But there is something I don’t understand. The Ab is liquid and it’s a very, very small amount (few ul). I checked the datasheet and it doesn’t say anything beside the recommendation to aliquot and store at -20/-80 upon arrival! Could you please clarify this to me? Do I have to dilute it in something?

Please let me know as soon as possible since I will need to use the Ab soon.

Thank you.

Best regards,

Read More
Answer

Thank you for your enquiry regarding ab48508.

Would you be so kind to confirm if the tube has been centrifuged before opening the lid? It would help me if you could specify how much volume of the liquid was in the vial?

Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.

Read More

Question

Inquiry: I am trying to find a primary antibody for 8-hydroxy-2'-deoxyguanosine by immunohistochemistry on paraffin sections with specific reactivity for mouse can you help me

Read More
Answer

Thank you for contacting us.

I can recommend the antibody ab48508 against 8-hydroxy-2'-deoxyguanosine. This antibody has been tested in immunohistochemistry on paraffin embedded tissue sections (IHC-P).
https://www.abcam.com/8-Hydroxy-2-deoxyguanosine-antibody-N451-ab48508.html (or use the following: https://www.abcam.com/8-Hydroxy-2-deoxyguanosine-antibody-N451-ab48508.html).

Alternatively, we do have also the ab264842 which has been tested in IHC-P.
https://www.abcam.com/8-Hydroxyguanosine-antibody-7D7E4-ab26842.html (or use the following: https://www.abcam.com/8-Hydroxyguanosine-antibody-7D7E4-ab26842.html).

All these antibodies will also work on mouse tissue. As they are however mouse antibodies, you must be careful not to introduce background by the detection with the secondary antibody. To avoid such a problem, I can recommend you to block endogenous mouse immunoglobulins on the tissue slides. This can be done with our MOM kit for example (ab127055). https://www.abcam.com/Mouse-on-Mouse-Polymer-IHC-Kit-ab127055.html (or use the following: https://www.abcam.com/Mouse-on-Mouse-Polymer-IHC-Kit-ab127055.html).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question

Customer called because their product only contained 25ul when this should ship with 500ul.

Read More
Answer

I am writing to you as I have been informed by our logistic group that ourAnti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1], AB48508, now ships with a concentration of 1.0mg/ml instead of 0.1mg/ml. As such the aliquot you receive will contain 50ug in a 50ul volume. Please let me know if this is alright to you and I will have the order released immediately.

This product is covered by our Abpromise guarantee. Should you experience any problems with this product we will replace or refund it under our Abpromise guarantee, if it is being used according to specifications listed on our datasheet.

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Read More

Question

Customer called because their product only contained 25ul when this should ship with 500ul.

Read More
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not parrive with the volume as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number XXXX. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

Read More

1-10 of 21 Abreviews or Q&A

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