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リコンビナントRabMAb

Anti-7-Methylguanosine 抗体 [EPR20846-92] (BSA and Azide free) (ab300741)

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Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)
  • Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)
  • FRET - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (ab300741)
  • Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20846-92] to 7-Methylguanosine - BSA and Azide free
  • Suitable for: Dot blot, FRET
  • Reacts with: Species independent

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free)
    7-Methylguanosine 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR20846-92] to 7-Methylguanosine - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Dot blot, FRETmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Chemical/ Small Molecule. Chemical/ Small Molecule corresponding to 7-methylguanosine (m7G) conjugated to a carrier

  • ポジティブ･コントロール

    • Dot Blot: HeLa total RNA and mRNA; m7G(cap)-oligo. FRET: m7G(cap)-oligo.

  • 特記事項

    ab300741 is the carrier-free version of ab300740.

     

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.20
    Constituent: 100% PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR20846-92

  • アイソタイプ

    IgG

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300741の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Dot blot
Use at an assay dependent concentration.
FRET
Use at an assay dependent concentration.
特記事項
Dot blot
Use at an assay dependent concentration.
FRET
Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    The 7-methylguanosine (m7G) cap, also known as N7-MeGppp is located on the 5'-terminus of the eukaryotic mRNA and in certain viral RNAs, lncRNA and pri-miRNAs.

  • 別名

    • m7G antibody

画像

  • Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)
    Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

    This data was developed using ab300740, the same antibody clone in a different buffer formulation.

    Concentration of ab300740: 1/500 dilution (1 μg/ml)

    Secondary ab: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/20000 dilution

    Blocking/diluting buffer and concentration: AdvanBlock

    Lane 1: HeLa total RNA

    Lane 2: HeLa mRNA

    Exposure time: 137s /17s

  • Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)
    Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

    This data was developed using ab300740, the same antibody clone in a different buffer formulation.

    Concentration of ab300740: 1/500 dilution (1 μg/ml)

    Secondary ab: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/20000 dilution

    Blocking/diluting buffer and concentration: AdvanBlock

    Lane 1: m7G(cap)-oligo

    Lane 2: m2A-oligo

    Lane 3: m1G-oligo

    Lane 4: m2, 2G-oligo

    Lane 5: G-oligo

    Lane 6: m2G-oligo

    Lane 7: m1A-oligo

    Lane 8: m6, 6A-oligo

    Exposure times: Lane 1: 6 seconds; Lanes 2-8: 180 seconds

  • FRET - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (ab300741)
    FRET - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (ab300741)

    This data was developed using ab300740, the same antibody clone in a different buffer formulation.

    A FRET-based assay was performed using 25 nm of each biotinylated oligo and ab300740 at a concentration range of 0.001-4.000 ug/ml.

  • Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)
    Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab300741 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab300741 は論文での使用が確認できていません。

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