AB300741

Anti-7-Methylguanosine 抗体 [EPR20846-92] (BSA and Azide free)

Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal 7-Methylguanosine antibody. Carrier free. Suitable for Dot, FRET and reacts with Modified Nucleic Acid samples.

別名を表示する

m7G

5 Images

Supplier Data

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

This data was developed using ab300740, the same antibody clone in a different buffer formulation. Concentration of ab300740 : 1/500 dilution (1 μg/ml) Secondary ab : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/20000 dilution Blocking/diluting buffer and concentration : AdvanBlock Lane 1 : HeLa total RNA Lane 2 : HeLa mRNA Exposure time : 137s /17s

Supplier Data

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

Supplier Data

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

This data was developed using ab300740, the same antibody clone in a different buffer formulation. Concentration of ab300740 : 1/500 dilution (1 μg/ml) Secondary ab : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/20000 dilution Blocking/diluting buffer and concentration : AdvanBlock Lane 1 : m7G(cap)-oligo Lane 2 : m2A-oligo Lane 3 : m1G-oligo Lane 4 : m2, 2G-oligo Lane 5 : G-oligo Lane 6 : m2G-oligo Lane 7 : m1A-oligo Lane 8 : m6, 6A-oligo Exposure times : Lane 1 : 6 seconds; Lanes 2-8 : 180 seconds

Fluorescence Resonance Energy Transfer - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

FRET

Supplier Data

Fluorescence Resonance Energy Transfer - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

This data was developed using ab300740, the same antibody clone in a different buffer formulation. A FRET-based assay was performed using 25 nm of each biotinylated oligo and ab300740 at a concentration range of 0.001-4.000 ug/ml.

Supplier Data

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

This data was developed using ab300740, the same antibody clone in a different buffer formulation. Concentration of ab300740 : 1/500 dilution (1 μg/ml) Secondary ab : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/20000 dilution Blocking/diluting buffer and concentration : AdvanBlock Lane 1 : m7G(cap)-oligo Lane 2 : m2A-oligo Lane 3 : m1G-oligo Lane 4 : m2, 2G-oligo Lane 5 : G-oligo Lane 6 : m2G-oligo Lane 7 : m1A-oligo Lane 8 : m6, 6A-oligo Exposure time : lane 1, 6 sec.; lanes 2-8, 180 sec.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR20846-92

アイソタイプ

IgG

キャリアフリー

Yes

アプリケーション

Dot, FRET

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "FRET" : {"fullname" : "Fluorescence Resonance Energy Transfer", "shortname":"FRET"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Nucleic Acid": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "FRET-species-checked": "testedAndGuaranteed", "FRET-species-dilution-info": "", "FRET-species-notes": "<p></p>" } } }

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製品の詳細

ab300741 is the carrier-free version of ab300740.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The target 7-Methylguanosine also known as 7-methylguanosine cap or 7-methylguanosine triphosphate (7-methylguanosine GTP) plays an important role in RNA processing. It is a modified nucleotide with a mass of approximately 362.3 Da. This cap structure is found at the 5' end of eukaryotic messenger RNA (mRNA). The 7-methylguanosine cap is expressed in the nucleus where it is added co-transcriptionally to nascent mRNA transcripts. This modification enhances mRNA stability and regulates its export to the cytoplasm.

Biological function summary

This cap modification promotes ribosomal binding facilitating efficient translation initiation. The 7-methylguanosine cap is part of the cap-binding complex which includes other proteins like eIF4E. This complex protects RNA from degradation and aids in splicing 5' end processing and polyadenylation. The methylation of the guanine base forms a structure that recruits proteins essential for mRNA maturation and function. Without this modification mRNA would display reduced translational efficiency and be more susceptible to exonucleolytic decay.

Pathways

7-methylguanosine participates in the RNA processing and translation initiation pathways. It interacts with the eukaryotic translation initiation factor 4E (eIF4E) a critical player in the regulation of mRNA cap-dependent translation. The binding of eIF4E to the 7-methylguanosine cap is essential for assembly of the eIF4F complex. This complex drives the mRNA to the ribosome facilitating protein synthesis. Additionally this cap structure influences mRNA export and poly(A) tail formation connecting to the broader mRNA metabolic pathway and impacting gene expression regulation.

Defects in the 7-methylguanosine cap can lead to improper mRNA processing and contribute to diseases like cancer and some congenital disorders. Dysregulation of cap-binding proteins such as eIF4E links to oncogenesis as these proteins can become overexpressed in cancers driving abnormal cell proliferation. Additionally cancer therapies often target these pathways to inhibit the translation of oncogenic mRNAs. Furthermore mutations or malfunctions in methylation enzymes can result in congenital developmental disorders as critical proteins necessary for normal growth and cellular function become deficient due to impaired mRNA translation.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information 7-Methylguanosine

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-7-Methylguanosine 抗体 [EPR20846-92] (BSA and Azide free)

Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free)

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

Fluorescence Resonance Energy Transfer - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

アプリケーション

免疫原

組合せ製品

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

Abcam product promise