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リコンビナントRabMAb

Anti-7-Methylguanosine 抗体 [EPR20846-92] (ab300740)

Reviews (1) Submit a question
Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (AB300740)
  • Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (AB300740)
  • FRET - Anti-7-Methylguanosine antibody [EPR20846-92] (ab300740)
  • Anti-7-Methylguanosine antibody [EPR20846-92] (AB300740)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20846-92] to 7-Methylguanosine
  • Suitable for: FRET, Dot blot
  • Reacts with: Species independent

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-7-Methylguanosine antibody [EPR20846-92]
    7-Methylguanosine 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR20846-92] to 7-Methylguanosine

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: FRET, Dot blotmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Chemical/ Small Molecule. Chemical/ Small Molecule corresponding to 7-methylguanosine (m7G) conjugated to a carrier

  • ポジティブ･コントロール

    • Dot Blot: HeLa total RNA and mRNA; m7G(cap)-oligo. FRET: m7G(cap)-oligo.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR20846-92

  • アイソタイプ

    IgG

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300740の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
FRET
Use at an assay dependent concentration.
Dot blot
1/500.
特記事項
FRET
Use at an assay dependent concentration.
Dot blot
1/500.

ターゲット情報

  • 関連性

    The 7-methylguanosine (m7G) cap, also known as N7-MeGppp is located on the 5'-terminus of the eukaryotic mRNA and in certain viral RNAs, lncRNA and pri-miRNAs.

  • 別名

    • m7G antibody

画像

  • Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (AB300740)
    Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (AB300740)

    Concentration of ab300740: 1/500 dilution (1 μg/ml)

    Secondary ab: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/20000 dilution

    Blocking/diluting buffer and concentration: AdvanBlock

    Lane 1: HeLa total RNA

    Lane 2: HeLa mRNA

    Exposure time: 137s /17s

  • Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (AB300740)
    Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (AB300740)

    Concentration of ab300740: 1/500 dilution (1 μg/ml)

    Secondary ab: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/20000 dilution

    Blocking/diluting buffer and concentration: AdvanBlock

    Lane 1: m7G(cap)-oligo

    Lane 2: m2A-oligo

    Lane 3: m1G-oligo

    Lane 4: m2, 2G-oligo

    Lane 5: G-oligo

    Lane 6: m2G-oligo

    Lane 7: m1A-oligo

    Lane 8: m6, 6A-oligo

    Exposure times: Lane 1: 6 seconds; Lanes 2-8: 180 seconds

  • FRET - Anti-7-Methylguanosine antibody [EPR20846-92] (ab300740)
    FRET - Anti-7-Methylguanosine antibody [EPR20846-92] (ab300740)

    A FRET-based assay was performed using 25 nm of each biotinylated oligo and ab300740 at a concentration range of 0.001-4.000 ug/ml.

  • Anti-7-Methylguanosine antibody [EPR20846-92] (AB300740)
    Anti-7-Methylguanosine antibody [EPR20846-92] (AB300740)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab300740 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab300740 は論文での使用が確認できていません。

レビューと Q&A

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レビューを送る 質問を送る

Western blot abreview for Anti-7-Methylguanosine antibody [EPR20846-92]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human (purified nucleoside/ cap analog 1nmol or 0.762 ug)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (no gel run - please see additional notes)
Loading amount
0.762 µg
Specification
purified nucleoside/ cap analog 1nmol or 0.762 ug
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Hsiang Chou

Verified customer

投稿 May 13 2024

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