AB245114

Anti-6X His tag® 抗体 [JPAR-2]

Anti-6X His tag® antibody [JPAR-2]

20ul selling size
RabMAb
Recombinant
Lab Essentials
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(4 Publications)

Anti-6X His tag® antibody [JPAR-2] (ab245114) is a rabbit monoclonal antibody detecting 6X His tag® in Western Blot, Flow Cytometry, IP, IHC-P.

- Biophysical QC for unrivalled batch-batch consistency

別名を表示する

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

4 Images

Flow Cytometry - Anti-6X His tag® antibody [JPAR-2] (AB245114)

Flow Cyt

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Flow Cytometry - Anti-6X His tag® antibody [JPAR-2] (AB245114)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a His-tagged Staphylococcus aureus cas9 (J7RUA5) construct labeling 6X His tag^® with ab245114 at 1/500 (Right) compared with a Rabbit monoclonal IgG isotype control (ab172730) (Left). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 647) (ab150079), at 1/2000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-6X His tag® antibody [JPAR-2] (AB245114)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-6X His tag® antibody [JPAR-2] (AB245114)

Immunohistochemical analysis of paraffin-embedded HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) labeling 6X His tag^® with ab245114 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on (A) HEK-293T transfected with a 6× His tag construct is observed. No staining on (B) HEK-293T transfected with an empty plasmid is observed. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunoprecipitation - Anti-6X His tag® antibody [JPAR-2] (AB245114)

His-tagged Staphylococcus aureus cas9 (J7RUA5; aa 1-1053; 125kDa) was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (transfected with Staphylococcus aureus cas9 (J7RUA5; aa 1-1053; 125kDa)) whole cell lysate with ab245114 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab245114 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1 : HEK-293T transfected with a His-tagged Staphylococcus aureus cas9 whole cell lysate 10 μg (Input).
Lane 2 : ab245114 IP in HEK-293T transfected with a His-tagged Staphylococcus aureus cas9 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab245114 in HEK-293T transfected with a His-tagged Staphylococcus aureus cas9 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-6X His tag® antibody [JPAR-2] (ab245114)

Observed band size: 125 kDa

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Supplier Data

Western blot - Anti-6X His tag® antibody [JPAR-2] (AB245114)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-6X His tag® antibody [JPAR-2] (ab245114) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty plasmid, whole cell lysate at 20 µg

Lane 2:

HEK-293T transfected with a His-tagged Staphylococcus aureus cas9 (J7RUA5; aa 1-1053; 125kDa) construct, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 125 kDa

false

Exposure time: 15s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

JPAR-2

アイソタイプ

IgG

キャリアフリー

アプリケーション

WB, IHC-P, Flow Cyt, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Tag": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-6X His tag® antibody [JPAR-2] (ab245114) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P). Tagged protein detection
6X His tag® antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [JPAR-2] also available for your convenience: ab245114, Carrier free - ab245203

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information 6X His tag®

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 29:e70800 PubMed40845179

2025

Decoy Receptor 2 as a Cell Cycle Arrest Biomarker for Predicting Renal Recovery Following Acute Kidney Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangling Yi,Liming Wang,Xiao-Yue Wang,Yu Fang,Jiarui Liu,Kehong Chen,Ya-Ni He,Jia Chen

Scientific reports 15:327 PubMed39747903

2025

FN1 shapes the behavior of papillary thyroid carcinoma through alternative splicing of EDB region.

Applications

Unspecified application

Species

Unspecified reactive species

Mian Liu,Pei Chen,Bo Wei,Hai-Long Tan,Ya-Xin Zhao,Lei Ai,Ning Li,Ying-Ke Jiang,Jing Lin,Shi-Jin Li,Shi Chang

Scientific reports 13:21086 PubMed38030755

2023

Advancing breast cancer diagnosis with a near-infrared fluorescence imaging smart sensor for estrogen/progesterone receptor detection.

Applications

Unspecified application

Species

Unspecified reactive species

Gong Zhang,Min Dong,Xiulei Yao,Yuke Xia,Han Yu,Yu Zhou,Chao Lian,Yunlei Zhang,Yiyao Cui

JCI insight 8: PubMed36480289

2023

ABT1 modifies SMARD1 pathology via interactions with IGHMBP2 and stimulation of ATPase and helicase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Gangadhar P Vadla,Sara M Ricardez Hernandez,Jiude Mao,Mona O Garro-Kacher,Zachary C Lorson,Ronin P Rice,Sarah A Hansen,Christian L Lorson,Kamal Singh,Monique A Lorson

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-6X His tag® 抗体 [JPAR-2]

Anti-6X His tag® antibody [JPAR-2]

Flow Cytometry - Anti-6X His tag® antibody [JPAR-2] (AB245114)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-6X His tag® antibody [JPAR-2] (AB245114)

Immunoprecipitation - Anti-6X His tag® antibody [JPAR-2] (AB245114)

Western blot - Anti-6X His tag® antibody [JPAR-2] (AB245114)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (4)

Abcam product promise