Anti-6X His tag® 抗体 [HIS-1] (ab49936)
Key features and details
- Mouse monoclonal [HIS-1] to 6X His tag®
- Suitable for: Flow Cyt (Intra), WB, Dot blot, ELISA, ICC/IF
- Reacts with: Species independent
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
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製品名
Anti-6X His tag® antibody [HIS-1]
6X His tag® 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [HIS-1] to 6X His tag® -
由来種
Mouse -
特異性
ab49939 preferentially reacts to recombinant proteins containing the 6X His tag® fused to amino terminus of fusion proteins.
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アプリケーション
適用あり: Flow Cyt (Intra), WB, Dot blot, ELISA, ICC/IFmore details -
種交差性
交差種: Species independent -
免疫原
Recombinant full length protein corresponding to 6X His tag®.
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特記事項
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR224242. Lot numbers higher than GR224242 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Proprietary Purification -
特記事項（精製）
Purified from Tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
HIS-1 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab49936の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| WB |
1/3000.
|
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| Dot blot |
Use at an assay dependent concentration.
|
|
| ELISA |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. |
|
WB
1/3000. |
|
Dot blot
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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関連性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
細胞内局在
Depends upon the localization of the parent protein tagged with hexahistidine. -
別名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab49936 は 3 報の論文で使用されています。
- Li T et al. SICKLE represses photomorphogenic development of Arabidopsis seedlings via HY5- and PIF4-mediated signaling. J Integr Plant Biol 64:1706-1723 (2022). PubMed: 35848532
- Allen ER et al. A Protective Monoclonal Antibody Targets a Site of Vulnerability on the Surface of Rift Valley Fever Virus. Cell Rep 25:3750-3758.e4 (2018). PubMed: 30590046
- Burgos JM & Schmitt MP The ChrSA and HrrSA Two-Component Systems Are Required for Transcriptional Regulation of the hemA Promoter in Corynebacterium diphtheriae. J Bacteriol 198:2419-30 (2016). PubMed: 27381918