Anti-6X His tag® 抗体 [AD1.1.10] (ab15149)
Key features and details
- Mouse monoclonal [AD1.1.10] to 6X His tag®
- Suitable for: ICC/IF, IHC-Fr, IP, WB
- Reacts with: Species independent
- Isotype: IgG1
Related conjugates and formulations
製品の概要
-
製品名
Anti-6X His tag® antibody [AD1.1.10]
6X His tag® 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [AD1.1.10] to 6X His tag® -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, IHC-Fr, IP, WBmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
特記事項
The clone number has been updated from (3H2201) to (AD1.1.10) both clone numbers name the same antibody clone.
HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.58% Sodium chloride, 95% Sodium phosphate -
Concentration information loading...
-
精製度
Protein G purified -
特記事項(精製)
Tissue cuture supernatant purified by Protein G affinity chromatography. -
一次抗体 備考
ab15149 recognises proteins containing the motif HHHHHH and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. -
ポリ/モノ
モノクローナル -
クローン名
AD1.1.10 -
ミエローマ
NS1 -
アイソタイプ
IgG1 -
研究分野
関連製品
-
Alternative Versions
- PerCP Anti-6X His tag® antibody [AD1.1.10] (ab117496)
- DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504)
- DyLight® 550 Anti-6X His tag® antibody [AD1.1.10] (ab117507)
- DyLight® 488 Anti-6X His tag® antibody [AD1.1.10] (ab117512)
- Biotin Anti-6X His tag® antibody [AD1.1.10] (ab15145)
- HRP Anti-6X His tag® antibody [AD1.1.10] (ab178563)
- PE Anti-6X His tag® antibody [AD1.1.10] (ab72467)
- SureLight® APC Anti-6X His tag® antibody [AD1.1.10] (ab72579)
- Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10] (ab81652)
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab15149の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
Use at an assay dependent concentration.
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
|
IP |
Use at an assay dependent concentration.
|
|
WB |
1/100 - 1/500.
|
特記事項 |
---|
ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
1/100 - 1/500. |
ターゲット情報
-
関連性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
細胞内局在
Depends upon the localization of the parent protein tagged with hexahistidine. -
別名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
画像
-
All lanes : Anti-6X His tag® antibody [AD1.1.10] (ab15149) at 0.5 µg/ml
Lane 1 : 60 kDa 6X His tagged protein at 0.3 µg
Lane 2 : 60 kDa 6X His tagged protein at 0.1 µg
Lane 3 : 60 kDa 6X His tagged protein at 0.03 µg
Lane 4 : 60 kDa 6X His tagged protein at 0.01 µg
Lane 5 : 60 kDa 6X His tagged protein at 0.003 µg
Secondary
All lanes : Goat Anti-Mouse (HRP) at 0.04 µg/mlImaged using West Pico chemiluminescent substrate.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (10)
ab15149 は 10 報の論文で使用されています。
- Medha et al. PE_PGRS45 (Rv2615c) protein of Mycobacterium tuberculosis perturbs mitochondria of macrophages. Immunol Cell Biol N/A:N/A (2023). PubMed: 37565603
- Ling H et al. A sugarcane smut fungus effector simulates the host endogenous elicitor peptide to suppress plant immunity. New Phytol 233:919-933 (2022). PubMed: 34716592
- Kishimoto H et al. The lipophilic cyclic peptide cyclosporin A induces aggregation of gel-forming mucins. Sci Rep 12:6153 (2022). PubMed: 35418571
- Yi KS et al. Broader neutralization of CT-P27 against influenza A subtypes by combining two human monoclonal antibodies. PLoS One 15:e0236172 (2020). PubMed: 32726321
- Lam M et al. Misfolded proteins bind and activate death receptor 5 to trigger apoptosis during unresolved endoplasmic reticulum stress. Elife 9:N/A (2020). PubMed: 31904339
- Moreland KT et al. In vitro calcite crystal morphology is modulated by otoconial proteins otolin-1 and otoconin-90. PLoS One 9:e95333 (2014). WB . PubMed: 24748133
- Smith MH et al. Misfolded proteins induce aggregation of the lectin Yos9. J Biol Chem 289:25670-7 (2014). WB . PubMed: 25086047
- Ma L et al. Serum anti-CCNY autoantibody is an independent prognosis indicator for postoperative patients with early-stage nonsmall-cell lung carcinoma. Dis Markers 35:317-25 (2013). WB . PubMed: 24167380
- Ma Z et al. Targeting human epidermal growth factor receptor signaling with the neuregulin's heparin-binding domain. J Biol Chem 284:32108-15 (2009). ICC/IF . PubMed: 19717564
- Bergman LM et al. Role of the unique N-terminal domain of CtBP2 in determining the subcellular localisation of CtBP family proteins. BMC Cell Biol 7:35 (2006). PubMed: 16999872