Anti-6X His tag® 抗体 (ab9108)
Key features and details
- Rabbit polyclonal to 6X His tag®
- Suitable for: WB, ICC/IF, ELISA, ChIP
- Reacts with: Species independent
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-6X His tag® antibody
6X His tag® 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to 6X His tag® -
由来種
Rabbit -
特異性
ELISA: Antibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH-BSA). A 1:25,000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction using HRP-conjugated Goat anti-Rabbit IgG at 1:20,000 and TMB as the substrate. Appropriate specificity controls were run. -
アプリケーション
適用あり: WB, ICC/IF, ELISA, ChIPmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide corresponding to 6X His tag® conjugated to keyhole limpet haemocyanin.
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ポジティブ･コントロール
- ICC/IF: CHO cells transfected with 12 tags constructs (CHO-12 tags) and HEK293 expressing His tag. WB: HeLa transfected with His-tagged gene, Epitope Tag Positive Control whole cell lysates, purified 12-Tag and cell lysates of CHO-12 Tags.
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特記事項
This antibody recognizes both C-terminal and N-terminal tags.
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077). Or search our wide range of secondary antibodies for use with your experiment.HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9108の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (14) |
Use a concentration of 1 µg/ml.
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| ICC/IF | (2) |
1/500 - 1/1000.
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| ELISA | (2) |
1/1000.
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| ChIP |
Use at an assay dependent concentration. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. |
|
ICC/IF
1/500 - 1/1000. |
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ELISA
1/1000. |
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ChIP
Use at an assay dependent concentration. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. |
ターゲット情報
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関連性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
細胞内局在
Depends upon the localization of the parent protein tagged with hexahistidine. -
別名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
画像
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Western blot - Anti-6X His tag® antibody (ab9108)All lanes : Anti-6X His tag® antibody (ab9108) at 1 µg/ml
Lane 1 : Purified 12-Tag at 2 µg
Lane 2 : Purified 12-Tag at 0.2 µg
Lane 3 : The cell lysates of CHO-12 Tags at 2 µg
Lane 4 : The cell lysates of CHO at 2 µg
Secondary
All lanes : Goat anti-rabbit, HRP conjugated at 0.2 µg/mlLane 1 and 4: Molecular weight Standards.
Primary and secondary antibody incubation at RT for 1 hour.
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Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody (ab9108)Immunofluorescent analysis of 4% paraformaldehyde (PFA) -fixed, permeabilized with 0.1% Triton X-100 in CHO cells transfected with 12 tags constructs (CHO-12 tags) labelling 6xHIS with ab9108 at 1/1000 dilution (1µg/mL), followed by Donkey Anti-rabbit IgG (H&L), Alexa Fluor® 594 antibody at 1/500 dilution (4 µg/mL) (Red). Nucleus was counterstained with DAPI (Blue). Bottom panel images showed Parallel staining in parental CHO cell line.
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Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody (ab9108)This image is courtesy of Vladimir Milenkovicab9108 staining CACNB3 with His tag expressed in HEK293 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/500 in 1% goat serum, 0.1% TX100 and PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/750).
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ChIP - Anti-6X His tag® antibody (ab9108)A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach). -
Western blot - Anti-6X His tag® antibody (ab9108)This image is courtesy of an anonymous AbreviewAll lanes : Anti-6X His tag® antibody (ab9108) at 1/4000 dilution
Lane 1 : HeLa whole cell lysate - transfected with empty vector
Lane 2 : HeLa whole cell lysate - transfected with His-tagged gene
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
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Western blot - Anti-6X His tag® antibody (ab9108)Use of ab9108 in western blotting at 1:1000 against Epitope Tag Positive Control lysate (ab4524).
Lane 1: 2 µl lysate
Lane 2: 1 µl lysate
Lane 3: 0.5 µl lysate
Lane 4: 0.25 µl lysate Use of ab9108 in western blotting at 1:1000 against E.coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 11 different epitope tag (ab5395). Lane 1: 2 µl lysate Lane 2: 1 µl lysate Lane 3: 0.5 µl lysate Lane 4: 0.25 µl lysate
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (165)
ab9108 は 165 報の論文で使用されています。
- Ma N et al. The F-box protein SHORT PRIMARY ROOT modulates primary root meristem activity by targeting SEUSS-LIKE protein for degradation in rice. J Integr Plant Biol 65:1937-1949 (2023). PubMed: 37013933
- Nagakubo T et al. Intracellular Phage Tail-Like Nanostructures Affect Susceptibility of Streptomyces lividans to Osmotic Stress. mSphere 8:e0011423 (2023). PubMed: 37039698
- Morano AA et al. A PPP-type pseudophosphatase is required for the maintenance of basal complex integrity in Plasmodium falciparum. Nat Commun 14:3916 (2023). PubMed: 37400439
- Hou C et al. Revisiting Fur Regulon Leads to a Comprehensive Understanding of Iron and Fur Regulation. Int J Mol Sci 24:N/A (2023). PubMed: 37240425
- Shin MJ et al. Protective effects of cell permeable Tat-PIM2 protein on oxidative stress induced dopaminergic neuronal cell death. Heliyon 9:e15945 (2023). PubMed: 37223703