Anti-6X His tag® 抗体 (ab9107)
Key features and details
- Chicken polyclonal to 6X His tag®
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Isotype: unknown
製品の概要
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製品名
Anti-6X His tag® antibody
6X His tag® 一次抗体 製品一覧 -
製品の詳細
Chicken polyclonal to 6X His tag® -
由来種
Chicken -
特異性
Antibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH-BSA). A 1:8000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction using Goat Anti Chicken IgY (IgG) and TMB as the substrate. Appropriate specificity controls were run. -
アプリケーション
適用あり: WB, ELISAmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide corresponding to 6X His tag® conjugated to keyhole limpet haemocyanin.
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特記事項
HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.1% Sodium azide -
Concentration information loading...
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精製度
IgY fraction -
特記事項(精製)
Antibodies were immunoaffinity purified using the peptide immobilized on a solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
unknown -
軽鎖の種類
unknown -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9107の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 1 kDa.
This antibody was an effective at 1:1000 (1 µg/ml) dilution using an affinity purified goat anti chicken IgY (IgG) HRP. The Westerns probed with this antibody are very sharp and clear with extremely low background and no non-specific binding. |
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ELISA |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 1 kDa. This antibody was an effective at 1:1000 (1 µg/ml) dilution using an affinity purified goat anti chicken IgY (IgG) HRP. The Westerns probed with this antibody are very sharp and clear with extremely low background and no non-specific binding. |
ELISA
Use at an assay dependent concentration. |
ターゲット情報
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関連性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
細胞内局在
Depends upon the localization of the parent protein tagged with hexahistidine. -
別名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab9107 は 1 報の論文で使用されています。
- Haas KT et al. Multitarget Immunohistochemistry for Confocal and Super-resolution Imaging of Plant Cell Wall Polysaccharides. Bio Protoc 10:e3783 (2020). PubMed: 33659438