AB134460

Anti-68kDa Neurofilament/NF-L 抗体

Name: Anti-68kDa Neurofilament/NF-L 抗体 (ab134460) | Abcam
Brand: Abcam Limited
SKU: AB134460
Price: 78000 JPY
Availability: InStock
Rating: 3 (1 reviews)

Anti-68kDa Neurofilament/NF-L antibody

(1 Review)

(2 Publications)

Chicken Polyclonal 68kDa Neurofilament/NF-L antibody. Suitable for IHC-FoFr, ICC/IF and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NEFL conjugated to Keyhole Limpet Haemocyanin.

別名を表示する

NF68, NFL, NEFL, Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody (AB134460)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody (AB134460)

Immunocytochemistry/Immunofluorescence analysis of Rat Neurons stained with chicken anti-NF-H (green). The NF-H antibody was used at a dilution of 1/100000 using our standard fixation and staining procedure (in protocol section).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody (AB134460)

Immunocytochemistry/Immunofluorescence analysis of dissociated adult mouse cerebral cortical cells labelling 68kDa Neurofilament/NF-L (green) with ab134460. Cells were fixed with 4% paraformaldehyde. NFL immunoreactivity is red and yellow, Blue - DAPI nuclear stain.

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-68kDa Neurofilament/NF-L antibody (AB134460)

IHC-FoFr

Unknown

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-68kDa Neurofilament/NF-L antibody (AB134460)

Immunohistochemical analysis of adult mouse brain labelling 68kDa Neurofilament/NF-L in axons in the white matter of the cerebellum with ab134460 at 1/1000 dilution (brown staining).

Key facts

宿主種

Chicken

クローン性

Polyclonal

アイソタイプ

IgY

キャリアフリー

交差種

Mouse

アプリケーション

ICC/IF, IHC-FoFr

applications

免疫原

Synthetic Peptide within Human NEFL conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P07196

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFoFr-species-checked": "testedAndGuaranteed", "IHCFoFr-species-dilution-info": "1/1000 - 1/2000", "IHCFoFr-species-notes": "<p>Use 2% paraformaldehyde-fixed tissues.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000 - 1/2000", "ICCIF-species-notes": "<p>Use 2% paraformaldehyde-fixed cells.</p>" } } }

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製品の詳細

Do not freeze the antibody unless you want to store it for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

バッファー組成

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The 68kDa Neurofilament also known as NF-L or neurofilament light plays a mechanical role in maintaining the structural integrity of neurons. This protein is characterized by its mass of 68 kilodaltons. It expresses specifically in the cytoplasm of neurons where it forms a critical part of the neurofilament protein triplet along with NF-M and NF-H. These components collectively form the intermediate filaments found in the neuron axons providing essential structural support.

Biological function summary

The neurofilament protein functions to stabilize the axonal diameter thereby affecting neuronal conductivity. It integrates into a complex with other neurofilament proteins and related molecules forming cross-bridges that aid in strengthening the cellular structure. This protein-protein interaction is vital for normal neuronal function and efficient nerve signal transmission.

Pathways

Neurofilament proteins contribute to cellular processes within the nervous system. It participates in the axonal transport pathway which includes the transport of synaptic vesicles and organelles along axons. This movement is facilitated by interactions with motor proteins like kinesin and dynein which help in moving these materials to their destinations. Additionally NF-L connects to actin filaments collaborating to sustain neuronal shape and function.

Neurofilament proteins have links to conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Elevated levels of NF-L are often observed in ALS suggesting neuronal damage as axonal injury raises the protein's presence in the cerebrospinal fluid. Similarly in Alzheimer's disease aberrant interaction of tau protein with neurofilament proteins disrupts neuronal function. These connections highlight NF-L's potential as a biomarker for neural injury and neurodegenerative diseases.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

See full target information NEFL

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Aging biology 1: PubMed38500536

2024

Early-Onset Hearing Loss in Mouse Models of Alzheimer's Disease and Increased DNA Damage in the Cochlea.

Applications

Unspecified application

Species

Unspecified reactive species

Jae-Hyeon Park,Burcin Duan Sahbaz,Komal Pekhale,Xixia Chu,Mustafa N Okur,Mhamed Grati,Kevin Isgrig,Wade Chien,Elena Chrysostomou,Lauren Sullivan,Deborah L Croteau,Uri Manor,Vilhelm A Bohr

Nature 605:152-159 PubMed35477759

2022

Neuroimmune cardiovascular interfaces control atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sarajo K Mohanta,Li Peng,Yuanfang Li,Shu Lu,Ting Sun,Lorenzo Carnevale,Marialuisa Perrotta,Zhe Ma,Benjamin Förstera,Karen Stanic,Chuankai Zhang,Xi Zhang,Piotr Szczepaniak,Mariaelvy Bianchini,Borhan R Saeed,Raimondo Carnevale,Desheng Hu,Ryszard Nosalski,Fabio Pallante,Michael Beer,Donato Santovito,Ali Ertürk,Thomas C Mettenleiter,Barbara G Klupp,Remco T A Megens,Sabine Steffens,Jaroslav Pelisek,Hans-Henning Eckstein,Robert Kleemann,Livia Habenicht,Ziad Mallat,Jean-Baptiste Michel,Jürgen Bernhagen,Martin Dichgans,Giuseppe D'Agostino,Tomasz J Guzik,Peder S Olofsson,Changjun Yin,Christian Weber,Giuseppe Lembo,Daniela Carnevale,Andreas J R Habenicht

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-68kDa Neurofilament/NF-L 抗体

Anti-68kDa Neurofilament/NF-L antibody

Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody (AB134460)

Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody (AB134460)

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-68kDa Neurofilament/NF-L antibody (AB134460)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

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