Anti-58K Golgi protein 抗体 - Golgi Marker (ab19072)
Key features and details
- Goat polyclonal to 58K Golgi protein - Golgi Marker
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-58K Golgi protein antibody - Golgi Marker
58K Golgi protein 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to 58K Golgi protein - Golgi Marker -
由来種
Goat -
アプリケーション
適用あり: WB, Flow Cytmore details -
種交差性
交差種: Human
交差が予測される動物種: Pig -
免疫原
Synthetic peptide:
SQLVECVPNFSEGKNQ
, corresponding to N terminal amino acids 2-17 of Human 58K Golgi protein. -
ポジティブ・コントロール
- Flow Cyt: HepG2 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab19072の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use a concentration of 0.03 - 0.2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).
A 1 hour primary incubation is recommended for this product. |
|
Flow Cyt |
Use a concentration of 10 µg/ml.
|
特記事項 |
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WB
Use a concentration of 0.03 - 0.2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa). A 1 hour primary incubation is recommended for this product. |
Flow Cyt
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.
Binds and promotes bundling of vimentin filaments originating from the Golgi. -
パスウェイ
Amino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.
One-carbon metabolism; tetrahydrofolate interconversion. -
関連疾患
Defects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA) [MIM:229100]; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities. -
配列類似性
In the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.
In the N-terminal section; belongs to the formiminotransferase family. -
細胞内局在
Cytoplasm > cytoskeleton > centrosome > centriole. Golgi apparatus. More abundantly located around the mother centriole. - Information by UniProt
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参照データベース
- Entrez Gene: 10841 Human
- Entrez Gene: 397517 Pig
- Omim: 606806 Human
- SwissProt: O95954 Human
- SwissProt: P53603 Pig
- Unigene: 415846 Human
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別名
- Formimidoyltetrahydrofolate cyclodeaminase antibody
- Formimidoyltransferase cyclodeaminase antibody
- Formiminotetrahydrofolate cyclodeaminase antibody
see all
画像
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Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Anti-58K Golgi protein antibody - Golgi Marker (ab19072) at 0.05 µg/ml + Human liver lysate at 35 µg
Predicted band size: 59 kDa
データシートおよび資料
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Datasheet download
参考文献 (7)
ab19072 は 7 報の論文で使用されています。
- Liang XH et al. Golgi-58K can re-localize to late endosomes upon cellular uptake of PS-ASOs and facilitates endosomal release of ASOs. Nucleic Acids Res 49:8277-8293 (2021). PubMed: 34244781
- Perland E et al. The Novel Membrane-Bound Proteins MFSD1 and MFSD3 are Putative SLC Transporters Affected by Altered Nutrient Intake. J Mol Neurosci 61:199-214 (2017). PubMed: 27981419
- Li H et al. Synaptic vesicles contain small ribonucleic acids (sRNAs) including transfer RNA fragments (trfRNA) and microRNAs (miRNA). Sci Rep 5:14918 (2015). WB . PubMed: 26446566
- Tinker JK et al. Purification and characterization of Yersinia enterocolitica and Yersinia pestis LcrV-cholera toxin A(2)/B chimeras. Protein Expr Purif 74:16-23 (2010). PubMed: 20438844
- Schrage YM et al. Aberrant heparan sulfate proteoglycan localization, despite normal exostosin, in central chondrosarcoma. Am J Pathol 174:979-88 (2009). IHC-P ; Human . PubMed: 19179614
- Willems SM et al. Myxoid tumours of soft tissue: the so-called myxoid extracellular matrix is heterogeneous in composition. Histopathology 52:465-74 (2008). IHC-P ; Human . PubMed: 18315599
- Zahn C et al. ADP-ribosylation Factor-like GTPase ARFRP1 Is Required for Trans-Golgi to Plasma Membrane Trafficking of E-cadherin. J Biol Chem 283:27179-27188 (2008). IHC ; Mouse . PubMed: 18662990