Anti-53BP2/ASPP2/BBP 抗体 (ab236448)
Key features and details
- Rabbit polyclonal to 53BP2/ASPP2/BBP
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-53BP2/ASPP2/BBP antibody
53BP2/ASPP2/BBP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to 53BP2/ASPP2/BBP -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IPmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment within Human 53BP2/ASPP2/BBP (N terminal). The exact sequence is proprietary.
Database link: Q13625 -
ポジティブ･コントロール
- WB: U-2 OS, mouse MEF and INS-1 cell lysates. IP: U-2 OS cell lysate. ICC/IF: U-2 OS cells transfected with a plasmid expressing 53BP2/ASPP2/BBP GFP.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab236448の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 126 kDa.
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| ICC/IF |
1/2000.
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| IP |
Use at an assay dependent concentration.
Use 3 µl to ~400 µg of extact |
| 特記事項 |
|---|
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WB
1/2000. Predicted molecular weight: 126 kDa. |
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ICC/IF
1/2000. |
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IP
Use at an assay dependent concentration. Use 3 µl to ~400 µg of extact |
ターゲット情報
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機能
Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42. -
組織特異性
Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines. -
配列類似性
Belongs to the ASPP family.
Contains 2 ANK repeats.
Contains 1 SH3 domain. -
ドメイン
The ankyrin repeats and the SH3 domain are required for a specific interactions with TP53. -
細胞内局在
Cytoplasm > perinuclear region. Nucleus. Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42. - Information by UniProt
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参照データベース
- Entrez Gene: 7159 Human
- Entrez Gene: 209456 Mouse
- Entrez Gene: 305025 Rat
- Omim: 602143 Human
- SwissProt: Q13625 Human
- SwissProt: Q8CG79 Mouse
- Unigene: 523968 Human
- Unigene: 287450 Mouse
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別名
- 53BP2 antibody
- Apoptosis stimulating of p53 protein 2 antibody
- Apoptosis stimulating protein of p53 2 antibody
see all
画像
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Western blot - Anti-53BP2/ASPP2/BBP antibody (ab236448)All lanes : Anti-53BP2/ASPP2/BBP antibody (ab236448) at 1/2000 dilution
Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate treated with control siRNA
Lane 2 : U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate treated with 53BP2/ASPP2/BBP siRNA
Lane 3 : Mouse MEF cell lysate
Lane 4 : Rat INS-1 cell lysate
Predicted band size: 126 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-53BP2/ASPP2/BBP antibody (ab236448)2% paraformaldehyde-fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells transfected with a plasmid expressing 53BP2/ASPP2/BBP GFP stained for 53BP2/ASPP2/BBP (red) using ab236448 at 1/2000 dilution in ICC/IF. GFP (green). Right panel shows the merged images.
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Immunocytochemistry/ Immunofluorescence - Anti-53BP2/ASPP2/BBP antibody (ab236448)2% paraformaldehyde-fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells transfected with a plasmid expressing 53BP2/ASPP2/BBP GFP stained for 53BP2/ASPP2/BBP (red) using ab236448 at 1/2000 dilution in ICC/IF. GFP (green). Lower panel shows the merged images.
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Immunoprecipitation - Anti-53BP2/ASPP2/BBP antibody (ab236448)53BP2/ASPP2/BBP was immunoprecipitated from ~400 µg U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate with with 3 μl ab236448. Western blot was performed from the immunoprecipitate using ab236448 at 1/2000 dilution.
Lane 1: U-2 OS cell lysate.
Lane 2: Control IP in U-2 OS cell lysate.
Lane 3: ab236448 IP in U-2 OS cell lysate.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab236448 は 1 報の論文で使用されています。
- Jiang Y et al. Phosphoproteomics Reveals AMPK Substrate Network in Response to DNA Damage and Histone Acetylation. Genomics Proteomics Bioinformatics N/A:N/A (2021). WB ; Mouse . PubMed: 33607295