Anti-53BP1 (phospho S25) 抗体 (ab70323)
Key features and details
- Rabbit polyclonal to 53BP1 (phospho S25)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-53BP1 (phospho S25) antibody
53BP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to 53BP1 (phospho S25) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human 53BP1 (phospho S25).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70323の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately >220 kDa (predicted molecular weight: 214 kDa).
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| IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately >220 kDa (predicted molecular weight: 214 kDa). |
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IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage. -
関連疾患
Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein. -
配列類似性
Contains 2 BRCT domains. -
翻訳後修飾
Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation. -
細胞内局在
Nucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks. - Information by UniProt
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参照データベース
- Entrez Gene: 7158 Human
- Entrez Gene: 27223 Mouse
- Omim: 605230 Human
- SwissProt: Q12888 Human
- SwissProt: P70399 Mouse
- Unigene: 440968 Human
- Unigene: 383499 Mouse
- Unigene: 481841 Mouse
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別名
- 53 BP1 antibody
- 53BP1 antibody
- FLJ41424 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 (phospho S25) antibody (ab70323)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma (left) and mouse teratoma (right) tissues labelling 53BP1 (phospho S24) with ab70323 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-53BP1 (phospho S25) antibody (ab70323)All lanes : Anti-53BP1 (phospho S25) antibody (ab70323) at 0.1 µg/ml
Lane 1 : Whole cell lysate from 293T cells, untreated
Lane 2 : Whole cell lysate from 293T cells treated
with NCS (200 ng/ml for 1h)
Lysates/proteins at 50 µg per lane.
Predicted band size: 214 kDa
Observed band size: >220 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab70323 は 5 報の論文で使用されています。
- Shi X et al. FLASH X-ray spares intestinal crypts from pyroptosis initiated by cGAS-STING activation upon radioimmunotherapy. Proc Natl Acad Sci U S A 119:e2208506119 (2022). PubMed: 36256824
- Callen E et al. 53BP1 Enforces Distinct Pre- and Post-resection Blocks on Homologous Recombination. Mol Cell 77:26-38.e7 (2020). PubMed: 31653568
- Guo H et al. Multi-omics analyses of radiation survivors identify radioprotective microbes and metabolites. Science 370:N/A (2020). PubMed: 33122357
- Lee V et al. Voriconazole enhances UV-induced DNA damage by inhibiting catalase and promoting oxidative stress. Exp Dermatol 29:29-38 (2020). PubMed: 31519066
- Mu F et al. Mangiferin induces radiosensitization in glioblastoma cells by inhibiting nonhomologous end joining. Oncol Rep 40:3663-3673 (2018). PubMed: 30272350