Anti-53BP1 抗体 (ab36823)
Key features and details
- Rabbit polyclonal to 53BP1
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-53BP1 antibody
53BP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to 53BP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, IPmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human 53BP1 aa 350-450.
Database link: Q12888 -
ポジティブ･コントロール
- IHC-P: Human prostate cancer tissue, mouse renal cancer tissue. WB: HeLa and mouse TCMK1 cell nuclear extract. IP: HEK293T whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab36823の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (4) |
1/10000 - 1/25000. Detects a band of approximately 220 kDa (predicted molecular weight: 214 kDa).
|
| IHC-P | (1) |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| IP |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
1/10000 - 1/25000. Detects a band of approximately 220 kDa (predicted molecular weight: 214 kDa). |
|
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage. -
関連疾患
Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein. -
配列類似性
Contains 2 BRCT domains. -
翻訳後修飾
Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation. -
細胞内局在
Nucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks. - Information by UniProt
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参照データベース
- Entrez Gene: 7158 Human
- Entrez Gene: 27223 Mouse
- Omim: 605230 Human
- SwissProt: Q12888 Human
- SwissProt: P70399 Mouse
- Unigene: 440968 Human
- Unigene: 383499 Mouse
- Unigene: 481841 Mouse
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別名
- 53 BP1 antibody
- 53BP1 antibody
- FLJ41424 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody (ab36823)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate cancer tissue, labeling CTCF with ab36823 at a 1/5000 dilution. Detection: DAB.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody (ab36823)Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse renal cancer tissue, labeling CTCF with ab36823 at a 1/1000 dilution. Detection: DAB.
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Western blot - Anti-53BP1 antibody (ab36823)All lanes : Anti-53BP1 antibody (ab36823) at 0.04 µg/ml
Lane 1 : HeLa cell nuclear extract at 50 µg
Lane 2 : HeLa cell nuclear extract at 5 µg
Lane 3 : TCMK1 mouse cell nuclear extract at 15 µg
Predicted band size: 214 kDa
Exposure time: 30 secondsDetection: Chemiluminescence. -
Immunoprecipitation - Anti-53BP1 antibody (ab36823)53BP1 was immunoprecipitated from 1 mg HEK293T whole cell lysate with ab36823 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab243868 at a 1/1000 dilution.
Lane 1: ab36823 IP in HEK293T whole cell lysate.
Lane 2: Contol IgG in HEK293T whole cell lysate.
Detection: Chemiluminescence.
Exposure time: 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (148)
ab36823 は 148 報の論文で使用されています。
- Kostova N et al. Effect of curcumin on γ-ray-induced cell response. J Radiat Res 64:471-479 (2023). PubMed: 36634350
- Benitez A et al. GEN1 promotes common fragile site expression. Cell Rep 42:112062 (2023). PubMed: 36729836
- Pelicci S et al. Correlative Multi-Modal Microscopy: A Novel Pipeline for Optimizing Fluorescence Microscopy Resolutions in Biological Applications. Cells 12:N/A (2023). PubMed: 36766696
- Jiang H et al. Human Endonuclease ANKLE1 Localizes at the Midbody and Processes Chromatin Bridges to Prevent DNA Damage and cGAS-STING Activation. Adv Sci (Weinh) 10:e2204388 (2023). PubMed: 36825683
- Sun C et al. NAD depletion mediates cytotoxicity in human neurons with autophagy deficiency. Cell Rep 42:112372 (2023). PubMed: 37086404