Anti-5-methylcytosine (5-mC) 抗体 [33D3] (ab10805)
Key features and details
- Mouse monoclonal [33D3] to 5-methylcytosine (5-mC)
- Suitable for: IHC-P, IP, Southern Blot, Flow Cyt, IHC-Fr
- Reacts with: Species independent
- Isotype: IgG1
製品の概要
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製品名
Anti-5-methylcytosine (5-mC) antibody [33D3]
5-methylcytosine (5-mC) 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [33D3] to 5-methylcytosine (5-mC) -
由来種
Mouse -
特異性
Modified base 5-methylcytidine found in DNA of plants and vertebrates.
Raised against the modified ribonucleoside. Specific for the presence of a methyl group on carbon 5 of the pyrimidine ring.
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アプリケーション
適用あり: IHC-P, IP, Southern Blot, Flow Cyt, IHC-Frmore details
適用なし: ICC -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule corresponding to 5-methylcytosine (5-mC).
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特記事項
Storage in frost-free freezers is not recommended. Should this product contain a precipitate microcentrifugation before use.While older lots have performed well in ICC, we have received inconsistent results with the latest lots. Unfortunately, we can no longer guarantee this antibody for use in ICC.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Constituents: PBS, 0.876% Sodium chloride -
Concentration information loading...
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精製度
Protein A purified -
一次抗体 備考
Ab10805 recognises the modified base 5-methylcytidine found in DNA of plants and vertebrates. DNA methylation is a DNA modification process, which is involved in the control of gene expression. Reports suggest that in tumours, DNA is frequently globally hypomethylated compared to the DNA from normal tissue. -
ポリ/モノ
モノクローナル -
クローン名
33D3 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10805の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (4) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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IP | (1) |
Use at an assay dependent concentration.
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Southern Blot |
1/200.
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Flow Cyt | (1) |
Use at an assay dependent concentration.
Use 10ul of working dilution to label 1000000 cells in 100ul. (see Habib, M. et al. (1999)). |
IHC-Fr |
Use at an assay dependent concentration.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
IP
Use at an assay dependent concentration. |
Southern Blot
1/200. |
Flow Cyt
Use at an assay dependent concentration. Use 10ul of working dilution to label 1000000 cells in 100ul. (see Habib, M. et al. (1999)). |
IHC-Fr
Use at an assay dependent concentration. |
ターゲット情報
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製品の状態
The native context of double-stranded DNA may obstruct antibody binding to 5-methylcytosine. For successful detection of 5-methylcytosine, we recommend that the DNA is denatured to make the nucleotides accessible for the antibody. Denaturing methods vary depending on each application. -
別名
- 5 m C antibody
- 5 mC antibody
- 5 me C antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805)This image is courtesy of an Abreview submitted by Ms Sara Maj Wätjen Hyldig
ab10805 staining 5-Methyl Cytidine in pig embryo (15 to 17 days) tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in paraformaldehyde, heat mediated antigen retrieval in Tris-EDTA buffer, permeabilization in Triton X-100 and blocking in 2% BSA for 10 minutes at 25°C. The primary antibody was diluted, 1/100 (PBS + 2% BSA) and incubated with sample for 1 hour at 25°C. An Alexa Fluor® 488 conjugated donkey polyclonal to mouse at 1/250 dilution, was used as secondary.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (177)
ab10805 は 177 報の論文で使用されています。
- Fang P et al. Self-Healing Electronics for Prognostic Monitoring of Methylated Circulating Tumor DNAs. Adv Mater 35:e2207282 (2023). PubMed: 36412926
- Wei X et al. Comprehensive analysis of transcriptomic profiling of 5-methylcytosin modification in placentas from preeclampsia and normotensive pregnancies. FASEB J 37:e22751 (2023). PubMed: 36692426
- Wang H et al. NSUN2-mediated M5c methylation of IRF3 mRNA negatively regulates type I interferon responses during various viral infections. Emerg Microbes Infect 12:2178238 (2023). PubMed: 36748584
- Yang WL et al. Nsun2 coupling with RoRγt shapes the fate of Th17 cells and promotes colitis. Nat Commun 14:863 (2023). PubMed: 36792629
- Cong W et al. Embryonic thermal manipulation leads growth inhibition and reduced hepatic insulin-like growth factor1 expression due to promoter DNA hypermethylation in broilers. Poult Sci 102:102562 (2023). PubMed: 36878101