AB10805

Anti-5-methylcytosine (5-mC) 抗体 [33D3]

Name: Anti-5-methylcytosine (5-mC) 抗体 [33D3] (ab10805) | Abcam
Brand: Abcam Limited
SKU: AB10805
Price: 60000 JPY
Availability: InStock
Rating: 5 (12 reviews)

Anti-5-methylcytosine (5-mC) antibody [33D3]

(12 Reviews)

(228 Publications)

Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) is a mouse monoclonal antibody detecting 5-methylcytosine (5-mC) in Flow Cytometry, IP, IHC-P, IHC-Fr.

- Over 170 publications
- Trusted since 2005

別名を表示する

5 Methycytosine, 5-Me Cytidine, 5-Me Cytosine, 5-Methyl-Cytidine, 5-Methyl-Cytosine, 5-MethylCytidine, 5-MethylCytosine, 5-mC, 5-meC, methyl CpG

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-methylcytosine (5-mC) antibody [33D3] (AB10805)

IHC-P

AbReview13478****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-methylcytosine (5-mC) antibody [33D3] (AB10805)

ab10805 staining 5-Methyl Cytidine in pig embryo (15 to 17 days) tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in paraformaldehyde, heat mediated antigen retrieval in Tris-EDTA buffer, permeabilization in Triton X-100 and blocking in 2% BSA for 10 minutes at 25°C. The primary antibody was diluted, 1/100 (PBS + 2% BSA) and incubated with sample for 1 hour at 25°C. An Alexa Fluor® 488 conjugated donkey polyclonal to mouse at 1/250 dilution, was used as secondary.

This image is courtesy of an Abreview submitted by Ms Sara Maj Wätjen Hyldig

IHC-P

CiteAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-methylcytosine (5-mC) antibody [33D3] (AB10805)

5-methylcytosine immunohistochemistry using Anti-5-methylcytosine (5-mC) antibody [33D3] ab10805. Publication image and figure legend from Wu, T., Zhang, Z. W., et al., 2020, Clin Epigenetics, PubMed : 31992357.

5hmC, 5mC, and Ki-67 index IHC staining in EPN_PF samples and normal cerebellum. a Representative image of 5hmC, 5mC, and Ki-67 staining in EPN_PF and cerebellum. b The Pearson correlation between nuclear positive cells of 5hmC and global 5hmC levels. c The Pearson correlation between Ki-67 index cells and global 5hmC levels. d The Pearson correlation between Ki-67 index and nuclear positive cells of 5hmC. Scale bars represent 100 μm

CiteAb

Dot Blot - Anti-5-methylcytosine (5-mC) antibody [33D3] (AB10805)

5-methylcytosin dot blot using Anti-5-methylcytosine (5-mC) antibody [33D3] ab10805. Publication image and figure legend from Yang, X., Yang, Y., et al., 2017,Cell Res, PubMed 28418038.

NSUN2 regulates nuclear-cytoplasmic shuttling and RNA-binding ability of ALYREF. (A) Immunofluorescence staining of ALYREF (red color) and ASF (green color) upon NSUN2 knockdown (top); line scan graphs (middle) and peak density quantification of line scan graphs (bottom) for ALYREF are also shown. Scale bar, 10 μm. Error bars indicate SEM (n = 120). (B) Western blotting (left) and quantification (right) of nuclear and cytoplasmic distribution of ALYREF in control and NSUN2-knockdown HeLa cells. The protein loading for the cytoplasmic fraction is about 2.5-fold higher than that for the nuclear fraction. PARP1 and TUBULIN serve as nuclear and cytoplasmic markers, respectively. Error bars indicate SEM (n = 3). (C) Immunofluorescence staining of ALYREF (red color) in NSUN2-knockdown HeLa cells transfected with control EGFP (EGFP-EV), EGFP-tagged siNSUN2-insensitive wild-type NSUN2 (EGFP-WT-Ins) or mutant (EGFP-DM-Ins) plasmids (top); line scan graphs (middle) and peak density quantification of line scan graphs (bottom) for ALYREF are also shown. Scale bar, 10 μm. Error bars indicate SEM (n = 120). (D) Western blotting (top) and quantification (bottom) of nuclear and cytoplasmic distribution of ALYREF in NSUN2-knockdown HeLa cells transfected with empty Myc expression vector (Myc-EV), Myc-tagged siNSUN2-insensitive wild-type NSUN2 (Myc-WT-Ins), or Mutant (Myc-DM-Ins). The protein loading for the cytoplasmic fraction is about 2.5-fold higher than that for the nuclear fraction. PARP1 and TUBULIN serve as nuclear and cytoplasmic markers, respectively. Error bars indicate SEM (n = 3). (E) PAR-CLIP assay (left) and quantification (right) of RNA pulled down by Flag-ALYREF upon NSUN2 knockdown. RNA labeled with biotin at 3' end of RNA (End Biotinylation Kit, Thermo) was visualized by the chemiluminescent nucleic acid detection module. m5C-modified RNAs were visualized by dot blotting using m5C antibody. Error bars indicate SEM (n = 3). (F) Rescue PAR-CLIP assay (left) and quantification (right) of RNA pulled down by Flag-ALYREF in NSUN2-knockdown HeLa cells transfected with Myc-EV, Myc-WT-Ins, or Myc-DM-Ins. RNA labeled with biotin at 3' end of RNA (End Biotinylation Kit, Thermo) was visualized by the Chemiluminescent nucleic acid detection module. Error bars indicate SEM (n = 3). P values were calculated by Student's t-test.

CiteAb

Immunohistochemistry - Anti-5-methylcytosine (5-mC) antibody [33D3] (AB10805)

5-methylcytosine immunohistochemistry-immunofluorescence using Anti-5-methylcytosine (5-mC) antibody [33D3] ab10805. Publication image and figure legend from Cao, Z., Hong, R., et al., 2017, PLoS One, Pubmed 28114389.

TSA and BIX-01294 treatment combined restores H3K9me2 and 5mC levels in SCNT embryos.SCNT embryos at the pronuclear stage were cultured in PZM-3 supplemented with 50 nM TSA or 50 nM TSA and 1 μm BIX-01294 for 24 h and SCNT embryos cultured in PZM-3 containing DMSO were served as NT control. Then DMSO, TSA and BIX-01294 in different groups were washed out and embryos were cultured in fresh PZM-3 medium until day7. We isolated and fixed the 4-cell and blastocysts from different groups including NT control, TSA treatment, TSA and BIX-01294 combination treatment for subsequent experiments. At the same time, in vivo fertilized (IVV) and in vitro fertilized (IVF) 4-cell and blastocysts were also collected and fixed. Finally, 4-cell embryos (A) and blastocysts (C) were stained for H3K9me2 (green) and DNA (propidium iodide, red). Bottom panels showed the merged images (yellow) between H3K9me2 signals (green) and DNA staining (red). (E) Blastocysts from all groups were stained for 5mC (green) and DNA (propidium iodide, red). Shown in A, C and E are representative Z-sections obtained by confocal microscopy from one experiment. White dash circle denotes ICM area in C and TE region in E, respectively. Scale bars = 50μm. (B, D) Quantification of the H3K9me2 fluorescence intensity in nucleus of 4-cell and trophectoderm cells. (F) Quantification of the 5mC fluorescence intensity in nucleus of trophectoderm cells. Data in B, D and F are shown as mean ± S.E.M. All experiments above were repeated three times with 20 embryos per group. Values with different superscripts across groups indicate significant differences (p < < 0.05) and *p < < 0.05.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

33D3

アイソタイプ

IgG1

軽鎖のタイプ

kappa

キャリアフリー

Yes

アプリケーション

IP, Flow Cyt, SB, IHC-P, IHC-Fr

applications

特異性

Modified base 5-methylcytidine found in DNA of plants and vertebrates.Raised against the modified ribonucleoside. Specific for the presence of a methyl group on carbon 5 of the pyrimidine ring.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "SB" : {"fullname" : "Southern Blot", "shortname":"SB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Nucleic Acid": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "While older lots have performed well in ICC, we have received inconsistent results with the latest lots. Unfortunately, we can no longer guarantee this antibody for use in ICC.", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "Use 10ul of working dilution to label 1000000 cells in 100ul. (see Habib, M. et al. (1999)). <a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "SB-species-checked": "guaranteed", "SB-species-dilution-info": "1/200", "SB-species-notes": "" } } }

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製品の詳細

Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) is a mouse monoclonal antibody and is validated for use in Flow Cyt, IHC-Fr, IHC-P, IP, SB in human samples.

Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) has been cited over 177 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) has high sensitivity and specificity.

Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) has 11 independent reviews from customers.

Anti-5-methylcytosine (5-mC) antibody [33D3] (ab10805) specifically detects 5-methylcytosine (5-mC) (UniProt ID: ; Molecular weight: kDa) and is sold in 50 µg selling sizes.

Storage in frost-free freezers is not recommended. Should this product contain a precipitate microcentrifugation before use.While older lots have performed well in ICC, we have received inconsistent results with the latest lots. Unfortunately, we can no longer guarantee this antibody for use in ICC.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Constituents: PBS, 0.876% Sodium chloride

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

5-methylcytosine often referred to as 5-mC 5-methyl cytosine or 5-methylcytidine is a methylated form of the nucleobase cytosine found in the DNA of various organisms. This modification typically occurs at the carbon 5 position of the cytosine ring contributing to the epigenetic regulation of gene expression. While the mass of 5-mC itself is relatively small since it is a modified nucleotide its impact on gene regulation is significant. 5-mC is widely expressed in the genome of higher eukaryotes particularly in CpG dinucleotides where it plays a role in mammalian development and cell differentiation.

Biological function summary

Cytosine methylation affects DNA-protein interactions leading to changes in chromatin structure and the regulation of gene activity. 5-methylcytosine is an important player in the chromatin modification complex where it collaborates with other epigenetic marks to control gene expression profiles. This modified nucleotide helps to stabilize gene silencing maintaining the closed chromatin state which inhibits the transcription machinery's access to specific genomic regions. Such silencing is vital during processes like X-chromosome inactivation and imprinting.

Pathways

Researchers have identified 5-mC within two critical pathways: DNA methylation and demethylation cycle and histone modification pathway. In the DNA methylation pathway 5-mC interacts closely with proteins like DNA methyltransferases (DNMTs) which directly add methyl groups to cytosine bases. In the context of histone modification 5-mC influences the binding of methyl-CpG-binding domain (MBD) proteins which read and interpret methylated DNA marks affecting histone modification and DNA accessibility.

The dysregulation of 5-methylcytosine patterns associates prominently with cancer and neurological disorders. Abnormal methylation patterns where 5-mC is either hypermethylated or hypomethylated can lead to the silencing of tumor suppressor genes or activation of oncogenes forming a basis for cancer development. In neurological disorders altered 5-mC levels link to conditions such as Rett syndrome where the dysfunction of the associated protein MeCP2 an important reader of 5-mC contributes to the disease pathology.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information 5-Methylcytosine

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文献 (228)

Recent publications for all applications. Explore the full list and refine your search

Clinical epigenetics 17:151 PubMed40993735

2025

ALYREF inhibits ferroptosis in vascular endothelial cells and improves atherosclerosis by epigenetic modification of CISD1.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghai Hua,Ling Yu,Wenjun Xiong,Ru Ying,Qiong Duan,Jianbing Zhu,Xiaoping Peng,Minzi Qiu

Biological & pharmaceutical bulletin 48:1343-1350 PubMed40930835

2025

Kaempferol Inhibits Ferroptosis in Lung Epithelial Cells in LPS-Induced Acute Lung Injury via m5C Methylation of TFRC.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Zhang,Weihua Wu,Peng An,Zhenfei Yu

Oncogene 44:3609-3624 PubMed40764753

2025

MUC1-C auto-regulatory complex with EBNA1 is responsible for latent Epstein-Barr virus-associated gastric cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroki Ozawa,Yin Wang,Henry G Withers,Naoki Haratake,Ayako Nakashoji,Atrayee Bhattacharya,Atsushi Fushimi,Chie Kikutake,Kazuhiro Yamanoi,Shaowen White,Keyi Wang,Tatsuaki Daimon,Keisuke Shigeta,Kazumasa Fukuda,Hirofumi Kawakubo,Yuko Kitagawa,Mark D Long,Benjamin E Gewurz,Donald Kufe

Frontiers in molecular neuroscience 18:1628860 PubMed40747242

2025

Tet3-mediated DNA demethylation is essential for maintaining the dedifferentiation capacity of mammalian Müller glia.

Applications

Unspecified application

Species

Unspecified reactive species

Erick J Martinez-Colin,Ivonne Lezama,Lenin D Ochoa-de la Paz,Monica Lamas

Journal of ovarian research 18:162 PubMed40707993

2025

5-methylcytosine regulated CCNL2 promotes tumorigenesis and cisplatin resistance of ovarian cancer with therapeutic implications.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Zhang,Guiyun Cheng,Wenwen Jiang,Beihua Kong,Shu Yao,Xihan Liu

Molecular cancer 24:201 PubMed40691844

2025

NSUN2-mediated cytosine-5 methylation of FSP1 protects acute myeloid leukemia cells from ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenle Ye,Yanchun Zhao,Yutong Zhou,Jiansong Huang,Xiao He,Zhixin Ma,Xin Huang,Chao Hu,Fenglin Li,Qing Ling,Huafeng Wang,Hongyan Tong,Jie Sun,Jie Jin

Animal bioscience 38:1973-1983 PubMed40241588

2025

Parental betaine supplementation promotes hepatic conversion of cholesterol to bile acids in offspring goslings with epigenetic modulation of CYP7A1 gene.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Ma,Liang Chen,Yan Wang,Lei Wu,Ruqian Zhao

Journal of experimental & clinical cancer research : CR 44:121 PubMed40234937

2025

LINC02167 stabilizes KSR1 mRNA in an mC-dependent manner to regulate the ERK/MAPK signaling pathway and promotes colorectal cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Junwen Qi,Tao Jiang,Bowen Liu,Qihang Hu,Junnan Chen,Ning Ma,Yixin Xu,Hu Song,Jun Song

Cancer communications (London, England) 45:846-879 PubMed40227950

2025

Novel molecular mechanisms of immune evasion in hepatocellular carcinoma: NSUN2-mediated increase of SOAT2 RNA methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jinhua Jiang,Feng Liu,Dan Cui,Caixia Xu,Jiachang Chi,Tinghua Yan,Fang Guo

Journal of translational medicine 23:385 PubMed40170133

2025

CircPRKCA facilitates esophageal squamous cell carcinoma metastasis via m5C-dependent CSF2 mRNA stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Lixia Wu,Lina Gu,Yang Zheng,Jingjing Liu,Zishuan Wei,Fei Liu,Jiali Li,Lingjiao Meng,Yang Sang,Meixiang Sang,Lianmei Zhao,Baoen Shan

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-5-methylcytosine (5-mC) 抗体 [33D3]

Anti-5-methylcytosine (5-mC) antibody [33D3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-methylcytosine (5-mC) antibody [33D3] (AB10805)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-methylcytosine (5-mC) antibody [33D3] (AB10805)

Dot Blot - Anti-5-methylcytosine (5-mC) antibody [33D3] (AB10805)

Immunohistochemistry - Anti-5-methylcytosine (5-mC) antibody [33D3] (AB10805)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

キャリアフリー

アプリケーション

特異性

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (228)

Abcam product promise