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AB214728

Anti-5-hydroxymethylcytosine (5-hmC) 抗体 [RM236]

Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236]

5

(1 Review)

|

(28 Publications)

Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236] (ab214728) is a rabbit monoclonal antibody detecting 5-hydroxymethylcytosine (5-hmC) in Flow Cytometry, IP, IHC-P, ICC/IF, ELISA, Dot Blot.

- Recombinant format for unrivalled batch-batch consistency
- Over 20 publications

別名を表示する

5-hmC

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236] (AB214728)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236] (AB214728)

Immunohistochemical analysis of paraffin-embedded human brain tissue labeling 5-hydroxymethylcytosine (5-hmC) with ab214728 at 1 μg/mL.

Flow Cytometry - Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236] (AB214728)
  • Flow Cyt

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Flow Cytometry - Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236] (AB214728)

Flow Cytometry analysis of 5-hmC expression in HEK293 cells using ab214728. The cells were fixed with ice-cold MeOH, permeabilized with 0.5% Triton X-100, denatured with 2N HCl, then stained with ab214728 (Blue) or with a negative control antibody (Red).

Immunocytochemistry/ Immunofluorescence - Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236] (AB214728)
  • ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236] (AB214728)

Immunocytochemical staining of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells using 0.5 μg/mL ab214728 (red). Actin filaments was labeled with fluorescein phalloidin (green). HeLa cells were fixed with 4% paraformaldehyde and permeabilized with methanol (−20 °C) before treatment with 2 N HCl for 30 minutes at 37°C to denature the DNA.

ELISA - Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236] (AB214728)
  • ELISA

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ELISA - Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236] (AB214728)

Direct ELISA of mouse brain genomic DNA using ab214728. The plate was directly coated with different concentrations of genomic DNA isolated from mouse brain tissue. 1 ug/mL or 3 ug/mL of ab214728 was used as the primary antibody and an HRP conjugated anti-rabbit IgG as the secondary antibody.

MeDIP - Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236] (AB214728)
  • MeDIP

Supplier Data

MeDIP - Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236] (AB214728)

hMeDIP was performed using anti-5-hmC antibody (RM236) at a 10 : 1 DNA : Ab ratio. 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) was spiked in 1ug of genomic DNA isolated from HeLa cells as the control. Realtime PCR was then performed to determine the capture of DNA standard as in % of input.

ELISA - Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236] (AB214728)
  • ELISA

Supplier Data

ELISA - Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236] (AB214728)

ELISA of single stranded DNA using ab214728 in a serial dilution. The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. Secondary antibody : alkaline phosphatase conjugated anti-rabbit IgG. ``

Dot Blot - Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236] (AB214728)
  • Dot

Supplier Data

Dot Blot - Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236] (AB214728)

Dot blot of double stranded DNA using ab214728 at 0.2 μg/mL. The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA. The pre-spotted membrane was then blotted with ab214728.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

RM236

アイソタイプ

IgG

キャリアフリー

No

アプリケーション

MeDIP, Flow Cyt, ELISA, IHC-P, ICC/IF, Dot

applications

特異性

ab214728 reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.

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Reactivity data

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製品の詳細

What is this antibody validated in?
Anti-5-hydroxymethylcytosine (5-hmC) antibody [RM236] (ab214728) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA, Dot Blot. Trusted by the scientific community
Anti-5-hydroxymethylcytosine (5-hmC) [RM236] (ab214728) was first used in a scientific publication in 2016 and has been cited over 20 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [RM236] also available for your convenience: ab214728, ab319976, Carrier free - ab323582
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

5-hydroxymethylcytosine (5-hmC) also known as hydroxymethylcytosine represents a significant epigenetic modification in DNA. This target with a mass of approximately 63.3 emerges through DNA demethylation processes where ten-eleven translocation (TET) enzymes oxidize 5-methylcytosine (5-mC). It is found largely in the brain where it shows high levels of expression but it can also appear in other tissues to a lesser extent. The modification is important in regulating gene expression and maintaining cellular identity.
Biological function summary

5-hydroxymethylcytosine plays a critical role in epigenetic regulation and serves as an intermediary in the active DNA demethylation process. It is not part of a stable complex but interacts transiently during epigenetic reconfiguration. The presence of 5-hmC marks regulatory regions of genes and is involved in transcriptional activation or repression depending on the cellular context. Its levels are dynamic influenced by various developmental stages and environmental conditions.

Pathways

This DNA modification is an important player in pathways related to cellular differentiation and development. It is integrally involved in the DNA demethylation pathway where TET enzymes mediate its formation from 5-methylcytosine. This pathway also involves other modifications leading to cytosine turnover linking 5-hmC to broader chromatin remodeling processes. Proteins interacting with 5-hmC include TET enzymes and other DNA maintenance proteins which highlight its role in maintaining genomic stability.

Aberrant 5-hmC levels show connection to several pathological states including cancer and neurological disorders. Altered hydroxymethylation patterns often associate with tumorigenesis particularly in gliomas where the misregulation of 5-hmC accompanies cancer progression. Neurological disorders like Rett syndrome also exhibit altered levels of 5-hmC implicating its role in neurodevelopmental processes. In these diseases mutations in proteins such as TET enzymes link to disrupted 5-hmC levels indicating its critical involvement in both oncogenesis and neurodevelopmental regulation.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

See full target information 5-hydroxymethylcytosine (5-hmC)
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文献 (28)

Recent publications for all applications. Explore the full list and refine your search

Journal of ovarian research 16:238 PubMed38102694

2023

Administration of adipose-derived mesenchymal stem cell conditioned medium improves ovarian function in polycystic ovary syndrome rats: involvement of epigenetic modifiers system.

Applications

Unspecified application

Species

Unspecified reactive species

Golnaz Shafiei,Mona Saheli,Sepideh Ganjalikhan-Hakemi,Tahereh Haghpanah,Seyed Noureddin Nematollahi-Mahani

PloS one 18:e0284809 PubMed37083878

2023

Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system.

Applications

Unspecified application

Species

Unspecified reactive species

Giuliana de Avila Ferronato,Carolina Mônica Dos Santos,Paola Maria da S Rosa,Alessandra Bridi,Felipe Perecin,Flávio Vieira Meirelles,Juliano Rodrigues Sangalli,Juliano Coelho da Silveira

Histochemistry and cell biology 159:513-526 PubMed37010548

2023

Impact of antigen retrieval protocols on the immunohistochemical detection of epigenetic DNA modifications.

Applications

Unspecified application

Species

Unspecified reactive species

Jobran M Moshi,Monique Ummelen,Jos L V Broers,Frans C S Ramaekers,Anton H N Hopman

International journal of molecular sciences 24: PubMed36768508

2023

Endometriosis of the Cervix: A Rare Clinical Case with the Possibility of Comparing the Eutopic and Ectopic Endometrium at the Cellular Level.

Applications

Unspecified application

Species

Unspecified reactive species

Konstantin A Toniyan,Elena Yu Gorbacheva,Valery V Boyarintsev,Irina V Ogneva

World journal of gastroenterology 28:5845-5864 PubMed36353202

2022

Expression of the methylcytosine dioxygenase ten-eleven translocation-2 and connexin 43 in inflammatory bowel disease and colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad El-Harakeh,Jessica Saliba,Kawthar Sharaf Aldeen,May Haidar,Layal El Hajjar,Mireille Kallassy Awad,Jana G Hashash,Margret Shirinian,Marwan El-Sabban

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e2201300 PubMed35892263

2022

NFAT1 Orchestrates Spinal Microglial Transcription and Promotes Microglial Proliferation via c-MYC Contributing to Nerve Injury-Induced Neuropathic Pain.

Applications

Unspecified application

Species

Unspecified reactive species

Bao-Chun Jiang,Ting-Yu Ding,Chang-Yun Guo,Xue-Hui Bai,De-Li Cao,Xiao-Bo Wu,Wei-Lin Sha,Ming Jiang,Long-Jun Wu,Yong-Jing Gao

International journal of endocrinology 2022:2658727 PubMed35755313

2022

TET3 Mediates 5hmC Level and Promotes Tumorigenesis by Activating AMPK Pathway in Papillary Thyroid Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiadong Chi,Wei Zhang,Yigong Li,Jie Zhao,Xiangqian Zheng,Ming Gao

Journal of asthma and allergy 15:645-664 PubMed35603013

2022

Food Allergy-Induced Autism-Like Behavior is Associated with Gut Microbiota and Brain mTOR Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Hua Cao,Hong-Juan He,Yuan-Yuan Zhao,Zhen-Zhen Wang,Xing-Yuan Jia,Kamal Srivastava,Ming-San Miao,Xiu-Min Li

Frontiers in oncology 12:856707 PubMed35480097

2022

Metformin Regulates TET2 Expression to Inhibit Endometrial Carcinoma Proliferation: A New Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Jingbo Zhang,Lei Kuang,Yanyu Li,Qing Wang,Hui Xu,Jianwei Liu,Xueyan Zhou,Yang Li,Bei Zhang

Bioengineered 13:6638-6649 PubMed35235761

2022

A novel epigenetic marker, Ten-eleven translocation family member 2 (TET2), is identified in the intractable epileptic brain and regulates ATP binding cassette subfamily B member 1 (ABCB1) in the blood-brain barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Fan-Cheng Kong,Li-Qin Lang,Jie Hu,Xia-Ling Zhang,Ming-Kang Zhong,Chun-Lai Ma
View all publications
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Abcam product promise

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保証に関する詳細については利用規約をご確認ください。
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