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Anti-5-HT2C Receptor 抗体 (ab32887)

Reviews (3)Q&A (2)References (3)
Western blot - Anti-5-HT2C Receptor antibody (ab32887)

    Key features and details

    • Goat polyclonal to 5-HT2C Receptor
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-5-HT2C Receptor antibody
      5-HT2C Receptor 一次抗体 製品一覧

    • 製品の詳細

      Goat polyclonal to 5-HT2C Receptor

    • 由来種

      Goat

    • 特異性

      Ab32887 recognises 5HT2C Receptor.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Rat, Dog, Chimpanzee

    • 免疫原

      Synthetic peptide:

      C-QVENLELPVN

      , corresponding to amino acids 437-446 of Human 5HT2C Receptor.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • ポジティブ･コントロール

      • Lysates of EBV immortalised Lymphoblastoids, T cell, MOLT4 and monocytic cell line U937.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab32887の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).

    1 hour primary incubation is recommended for this product.
    特記事項
    WB
    Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).

    1 hour primary incubation is recommended for this product.

    ターゲット情報

    • 機能

      This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

    • 配列類似性

      Belongs to the G-protein coupled receptor 1 family.

    • ドメイン

      The PDZ domain-binding motif is involved in the interaction with MPDZ.

    • 翻訳後修飾

      N-glycosylated.

    • 細胞内局在

      Cell membrane.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • 5 Hydroxytryptamine 2C receptor antibody
      • 5-HT-1C antibody
      • 5-ht-1c receptor antibody
      • 5-HT-2C antibody
      • 5-HT1C antibody
      • 5-HT2C antibody
      • 5-HTR2C antibody
      • 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled antibody
      • 5-hydroxytryptamine receptor 1C antibody
      • 5-hydroxytryptamine receptor 2C antibody
      • 5HT1C antibody
      • 5HT2C antibody
      • 5HT2C_HUMAN antibody
      • 5HTR2C antibody
      • 5Hydroxytryptamine 2C receptor antibody
      • Htr1c antibody
      • HTR2C antibody
      • serotonin 1c receptor antibody
      • serotonin 2c receptor antibody
      • Serotonin 5-HT-2C receptor antibody
      • Serotonin receptor 2C antibody
      see all

    画像

    • Western blot - Anti-5-HT2C Receptor antibody (ab32887)
      Western blot - Anti-5-HT2C Receptor antibody (ab32887)
      Anti-5-HT2C Receptor antibody (ab32887) at 1 µg/ml + EBV immortalised Lymphoblastoid lysate in RIPA buffer at 35 µg

      Developed using the ECL technique.

      Predicted band size: 52 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute

    参考文献 (3)

    ab32887 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab32887 は 3 報の論文で使用されています。

    • Price AE  et al. Endogenous Serotonin 5-HT2A and 5-HT2C Receptors Associate in the Medial Prefrontal Cortex. ACS Chem Neurosci 10:3241-3248 (2019). PubMed: 30645940
    • Delicata F  et al. Preferential modulation of the lateral habenula activity by serotonin-2A rather than -2C receptors: Electrophysiological and neuroanatomical evidence. CNS Neurosci Ther 24:721-733 (2018). PubMed: 29479825
    • Ji G  et al. 5-HT2CReceptor Knockdown in the Amygdala Inhibits Neuropathic-Pain-Related Plasticity and Behaviors. J Neurosci 37:1378-1393 (2017). PubMed: 28011743

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-5HT2C Receptor antibody

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    投稿 Dec 04 2012

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-5HT2C Receptor antibody

     Inconclusive
    Abreviews
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Fixative
    formalin
    Antigen retrieval step
    Enzymatic
    Permeabilization
    Yes - 1xPBT
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 17°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Sep 04 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-5HT2C Receptor antibody

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (hypothalamus PVN sections)
    Specification
    hypothalamus PVN sections
    Fixative
    ethanol 70%
    Permeabilization
    Yes - triton-x
    Blocking step
    2% BSA 2% NGS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Ms. Rebecca Ring

    Verified customer

    投稿 Sep 30 2011

    Question

    Do you have the blocking peptide available for this antibody?

    Read More
    Answer

    Thank you for your inquiry.

    Great news! The blocking peptide has been added as 5HT2C Receptor peptide ab168550.

    https://www.abcam.com/5ht2c-receptor-peptide-ab168550.html

    I hope this information helps. Please contact us with any other questions.

    Read More

    Question

    I would like to test ab32887 and ab44640 in IHC-FoFr and WB, respectively, which are two untested applications.

    Read More
    Answer


    I am very pleased to hear you would like to accept our offer and test ab32887 and ab44640 in IHC-FoFr and WB, respectively. These codes will give you: 1 free primary antibody each before the expiration date. To redeem this offer, please submit an Abreview for IHC-FoFr and WB and include these codes in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The codes will be active once the Abreviews have been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com