Anti-4 Hydroxynonenal 抗体 [HNEJ-2] (ab48506)
![Western blot - Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506)](https://www.abcam.co.jp/ps/products/48/ab48506/Images/ab48506-532358-anti-4-hydroxynonenal-antibody-hnej-2-western-blot-bsa-bsa-4hydroxynonenal-bsa-4hydroxynonenal-bsa.jpg "Western blot - Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506)")
Key features and details
- Mouse monoclonal [HNEJ-2] to 4 Hydroxynonenal
- Suitable for: WB, IHC-P, IHC-FoFr
- Reacts with: Species independent
- Isotype: IgG1
製品の概要
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製品名
Anti-4 Hydroxynonenal antibody [HNEJ-2]
4 Hydroxynonenal 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [HNEJ-2] to 4 Hydroxynonenal -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, IHC-FoFrmore details -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule corresponding to 4 Hydroxynonenal conjugated to keyhole limpet haemocyanin.
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ポジティブ･コントロール
- Colorectal carcinoma cells.
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特記事項
This product was changed from ascites to tissue culture supernatant on 28th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Constituents: 0.02% Potassium chloride, 0.02% Monobasic dihydrogen potassium phosphate, 0.0125% Sodium chloride, 0.115% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Purified from serum-free cell culture medium by ammonium sulfate purification followed by protein A purification. -
ポリ/モノ
モノクローナル -
クローン名
HNEJ-2 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab48506の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/1000.
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| IHC-P | (3) |
1/25.
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| IHC-FoFr | (1) |
1/25.
|
| 特記事項 |
|---|
|
WB
1/1000. |
|
IHC-P
1/25. |
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IHC-FoFr
1/25. |
ターゲット情報
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関連性
Aldehydic products of lipid peroxidation, such as 4 hydroxynonenal (4 HNE), have been implicated in the etiology of pathological changes under oxidative stress as a key mediator of oxidative stress induced cell death. It is a stable product of lipid peroxidation, is proarrhythmic and may contribute to the cytotoxic effects of oxidative stress. -
細胞内局在
Cytoplasmic -
別名
- 4 HNE antibody
- 4-Hydroxy-2-Nonenal antibody
画像
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Western blot - Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506)All lanes : Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506) at 1/1000 dilution
Lane 1 : BSA cell lysate at 0.5 µg
Lane 2 : BSA cell lysate at 1 µg
Lane 3 : 4-Hydroxynonenal (BSA) cell lysate at 0.5 µg
Lane 4 : 4-Hydroxynonenal (BSA) cell lysate at 1 µg
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 66 kDa why is the actual band size different from the predicted?Western blot: Anti-4-HNE antibody [HNEJ-2] (ab48506) staining at 1/1000 dilution, shown in black. In Western blot, ab48506 was shown to bind specifically to 4-HNE. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with a high-sensitivity ECL substrate kit and imaged with 3 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Mouse (H+L) 5000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506)](https://www.abcam.co.jp/ps/products/48/ab48506/Images/ab48506-334541-anti-4-hydroxynonenal-antibody-hnej-2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506)Lu, L. et al PLoS One. 2015 Dec 3;10(12):e0144068. doi: 10.1371/journal.pone.0144068. eCollection 2015. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Elevated brain oxidative damage in ischemic 5xFAD mice
Formalin-fixed, paraffin-embedded mouse brain tissue (from non-trasngenic or 5xFAD transgenic animals) stained for 4 Hydroxynonenal using ab48506 at in immunohistochemical analysis.
Scale bar = 20μm. (A2) and (B2) are representative images of 4-HNE staining (Red) in brain cortex and CA1 region, respectively.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506)](https://www.abcam.co.jp/ps/products/48/ab48506/Images/ab48506-61015-anti-4-hydroxynonenal-antibody-hnej-2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506)This image was kindly supplied by Dr Jinqing Li by Abreviewab48506 at a 1/25 dilution staining 4-Hydroxy-2-Nonenal in mouse heart tissue sections by Immunohistochemistry (paraffin embedded) incubated for 15 hours at +4°C. Fixed with formaldehyde, heat mediated antigen retrieval step performed using citrate buffer. Blocked using 5% serum for 20 minutes at 20°C. Secondary used undiluted polyclonal Goat anti-mouse IgG conjugated to Alexa Fluor 594. -
![Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506)](https://www.abcam.co.jp/ps/products/48/ab48506/Images/ab48506-61014-anti-4-hydroxynonenal-antibody-hnej-2-immunohistochemistry.jpg)
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-4 Hydroxynonenal antibody [HNEJ-2] (ab48506)This image is courtesy of an Abreview submitted by Seontae Kimab15463 staining 4-Hydroxynonenal in the Mouse melanoma cancer tissue sections by IHC-FoFr (PFA perfusion fixed frozen Sections). Tissue samples were fixed with 4% Formalin, Antigen retrieval carried out heat mediation using anautoclave for 10 minutes, permeabilized using 0.1% SDS and blocked with 1% BSAfor 15 minutes at 25°C. The sample was incubated with primary antibody (1/25 in PBS + 1% BSA) at 4°C for 12 hours.An Alexa Fluor®488-conjugated Goat anti-mouse IgG(H+L) monoclonal(1/100) was used as the secondary antibody.
データシートおよび資料
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Datasheet download
参考文献 (203)
ab48506 は 203 報の論文で使用されています。
- Xu J et al. Targeted transplantation of engineered mitochondrial compound promotes functional recovery after spinal cord injury by enhancing macrophage phagocytosis. Bioact Mater 32:427-444 (2024). PubMed: 37954465
- Kumar S et al. Low dose radiation upregulates Ras/p38 and NADPH oxidase in mouse colon two months after exposure. Mol Biol Rep 50:2067-2076 (2023). PubMed: 36542238
- Zhang J et al. Direct targeting of sEH with alisol B alleviated the apoptosis, inflammation, and oxidative stress in cisplatin-induced acute kidney injury. Int J Biol Sci 19:294-310 (2023). PubMed: 36594097
- Xu X et al. Increased ATF2 expression predicts poor prognosis and inhibits sorafenib-induced ferroptosis in gastric cancer. Redox Biol 59:102564 (2023). PubMed: 36473315
- Shan G et al. Inhibition of PKA/CREB1 pathway confers sensitivity to ferroptosis in non-small cell lung cancer. Respir Res 24:277 (2023). PubMed: 37957645
