Anti-4-1BBL 抗体 (ab64912)
Key features and details
- Rabbit polyclonal to 4-1BBL
- Suitable for: ELISA, WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-4-1BBL antibody
4-1BBL 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to 4-1BBL -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human 4-1BBL (internal sequence).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64912の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| WB |
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 27 kDa).
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| ICC/IF |
1/500 - 1/1000.
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| IHC-P |
Use at an assay dependent concentration. PubMed: 25409517
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| 特記事項 |
|---|
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ELISA
Use at an assay dependent concentration. |
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WB
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 27 kDa). |
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ICC/IF
1/500 - 1/1000. |
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IHC-P
Use at an assay dependent concentration. PubMed: 25409517 |
ターゲット情報
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機能
Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages. -
組織特異性
Expressed in brain, placenta, lung, skeletal muscle and kidney. -
配列類似性
Belongs to the tumor necrosis factor family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8744 Human
- Omim: 606182 Human
- SwissProt: P41273 Human
- Unigene: 1524 Human
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別名
- 4 1BB L antibody
- 4 1BB ligand antibody
- 4 1BBL antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-4-1BBL antibody (ab64912)
ab64912, at a 1/500 dilution, staining Human 4-1BBL in HeLa cells, by Immunofluorescence, with (+) or without (-) immunising peptide.
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Western blot - Anti-4-1BBL antibody (ab64912)All lanes : Anti-4-1BBL antibody (ab64912) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells
Lane 2 : Extracts from HUVEC cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 27 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab64912 は 6 報の論文で使用されています。
- Ji Z et al. Single-Cell RNA-Sequencing Reveals Peripheral T Helper Cells Promoting the Development of IgG4-Related Disease by Enhancing B Cell Activation and Differentiation. Int J Mol Sci 24:N/A (2023). PubMed: 37762039
- Fournier B et al. Inherited TNFSF9 deficiency causes broad Epstein-Barr virus infection with EBV+ smooth muscle tumors. J Exp Med 219:N/A (2022). PubMed: 35657354
- Cui Z et al. CD47 blockade enhances therapeutic efficacy of cisplatin against lung carcinoma in a murine model. Exp Cell Res 405:112677 (2021). PubMed: 34111474
- Eirin A et al. Metabolic Syndrome Interferes with Packaging of Proteins within Porcine Mesenchymal Stem Cell-Derived Extracellular Vesicles. Stem Cells Transl Med 8:430-440 (2019). PubMed: 30707002
- Yoshimori M et al. CD137 expression is induced by Epstein-Barr virus infection through LMP1 in T or NK cells and mediates survival promoting signals. PLoS One 9:e112564 (2014). IHC-P . PubMed: 25409517
- Dumitriu IE et al. High levels of costimulatory receptors OX40 and 4-1BB characterize CD4+CD28null T cells in patients with acute coronary syndrome. Circ Res 110:857-69 (2012). PubMed: 22282196