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AB231795

Anti-3-methylcytosine 抗体

Anti-3-methylcytosine antibody

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(2 Publications)

Rabbit Polyclonal 3-methylcytosine antibody. Suitable for Dot and reacts with Synthetic peptide samples. Cited in 2 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to 3-methylcytosine.
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Dot Blot - Anti-3-methylcytosine antibody (AB231795)
  • Dot

Supplier Data

Dot Blot - Anti-3-methylcytosine antibody (AB231795)

To demonstrate the specifcity of ab231795 a Dot Blot analysis was performed using synthetic oligonucleotides containing different modifed bases.

150 and 50 pmol of the respective oligo's were spotted on the membrane. ab231795 was diluted 1/400 in PBS-T containing 10 % skimmed milk and 1% BSA.

ab231795 shows a high specificity of the antibody for the oligonucleotide with the 3-methylcytosine modification.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

アプリケーション

Dot

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein G
バッファー組成
Preservative: 0.05% Sodium azide, 0.05% Proclin 300 Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

3-methylcytosine also known as 3mC is a modified nucleobase in DNA where a methyl group gets added to the cytosine ring. This process involves methyltransferase enzymes and 3-methylcytosine is smaller in mass compared to other methylated cytosines. Detailed mass data is not always available but it is lighter than 5-methylcytosine. 3mC expresses in diverse tissue types indicating its broad biological relevance. Its formation can influence gene expression and genomic stability therefore impacting cellular function.
Biological function summary

3-methylcytosine contributes to the regulation of DNA structure and accessibility. It does this by affecting the interaction between DNA and protein complexes like chromatin. The presence of 3mC may act as a signal for the recruitment or inhibition of certain proteins involved in DNA repair and transcriptional regulation. While it forms part of complex regulatory networks individual 3mC modifications can have significant impacts on gene activity.

Pathways

3-methylcytosine plays a role in epigenetic regulation mechanisms that control gene expression without altering DNA sequence. One key pathway involving 3mC is the DNA damage response pathway where it can affect the recognition and repair of damaged DNA. Proteins like DNA methyltransferases and TET enzymes interact with 3-methylcytosine reflecting its part in maintaining genome integrity and regulating cellular responses to DNA methylation changes.

Alterations in 3-methylcytosine patterns associate with cancer and neurodegenerative diseases. In cancer aberrant methylation can lead to the silencing of tumor suppressor genes contributing to tumorigenesis. Proteins such as DNMT (DNA methyltransferases) are importantly involved in these epigenetic changes often found deregulated in malignancies. In neurodegenerative disorders unusual 3mC presence can disrupt normal neuronal gene expression potentially linked through pathways involving histone modifications and their regulatory proteins.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

See full target information 3-methylcytosine
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed38095307

2023

Steroidogenesis and androgen/estrogen signaling pathways are altered in in vitro matured testicular tissues of prepubertal mice.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Moutard,Caroline Goudin,Catherine Jaeger,Céline Duparc,Estelle Louiset,Tony Pereira,François Fraissinet,Marion Delessard,Justine Saulnier,Aurélie Rives-Feraille,Christelle Delalande,Hervé Lefebvre,Nathalie Rives,Ludovic Dumont,Christine Rondanino

Nucleic acids research 47:11729-11745 PubMed31642493

2019

Human RAD51 paralogue RAD51C fosters repair of alkylated DNA by interacting with the ALKBH3 demethylase.

Applications

Unspecified application

Species

Unspecified reactive species

Monisha Mohan,Deepa Akula,Arun Dhillon,Arun Goyal,Roy Anindya
View all publications
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組合せ製品

Sample Prep & Detection Kits

AB218260

Oligonucleotide Conjugation Kit

Conjugation - Oligonucleotide Conjugation Kit (AB218260)

  • 0
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Primary Antibodies

AB10805

Anti-5-methylcytosine (5-mC) antibody [33D3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-methylcytosine (5-mC) antibody [33D3] (AB10805)

  • 5
  • 12
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB214727

Anti-5-methylcytosine (5-mC) antibody [RM231]

Recombinant

Immunocytochemistry - Anti-5-methylcytosine (5-mC) antibody [RM231] (AB214727)

  • 0
  • 0

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