AB211495

Anti-2-methyladenosine (m2A) 抗体 [EPR -19851-59]

Anti-2-methyladenosine (m2A) antibody [EPR -19851-59]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal 2-methyladenosine (m2A) antibody. Suitable for IP, ELISA and reacts with Modified Nucleic Acid samples. Cited in 1 publication.

別名を表示する

2-methyladenosine, m2A

2 Images

ELISA

Supplier Data

ELISA - Anti-2-methyladenosine (m2A) antibody [EPR -19851-59] (AB211495)

Biotinylated m2A (modified) and A (unmodified) oligonucleotides with the below sequence were coated onto wells of a 96 well plate.

Modified oligonucleotide (5 μM) 5' Biotin-mN.mN.mN.mN.mN.[ m2A]*.mN.mN.mN.mN.mN 3'

Unmodified oligonucleotide (5 μM) 5' Biotin-mN.mN.mN.mN.mN.[A]*.mN.mN.mN.mN.mN 3'

N - equimolar mixture of (A/U/G/C)
m - 2'O methyl protection
* - phosphorothioate protection

ELISA was performed on 1.0 μg/ml of antigen using ab211495 at a concentration range of 0.005-4.000 μg/ml followed by Goat Anti-Rabbit IgG (H+L) alkaline phosphatase conjugated secondary antibody at 1/2500 dilution.

Supplier Data

Immunoprecipitation - Anti-2-methyladenosine (m2A) antibody [EPR -19851-59] (AB211495)

The IP was performed in a U-bottom non-adsorbing propylene 96-well plate.

ab211495 (0.2 μg) was coated into Dynabeads® sheep-anti-rabbit IgG (50 μl) for 1h at RT.

Unmodified/modified oligonucleotides (5 μM) were added to samples containing the antibody/bead complexes and incubated with agitation for 1 hour at RT.

After washing, Peroxidase-conjugated Streptavidin was incubated at 1/1000 dilution with agitation for 1 hour at RT.

ECL substrate was then added and the results read in a non-transparent 96-well plate with a digital detector and analyzed using ImageJ.

Lane 1 : Buffer only.

Lane 2 : Modified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[ m2A]*.mN.mN.mN.mN.mN 3'

Lane 3 : Unmodified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[A]*.mN.mN.mN.mN.mN 3'

N - equimolar mixture of (A/U/G/C)

m - 2'O methyl protection

* - phosphorothioate protection

Blocking buffer and concentration : 5% NFDM/TBST

Dilution buffer and concentration : TBST/0.1% Triton X-100/1 mM EDTA

All lanes:

Immunoprecipitation - Anti-2-methyladenosine (m2A) antibody [EPR -19851-59] (ab211495)

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Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR -19851-59

アイソタイプ

IgG

キャリアフリー

アプリケーション

ELISA, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

Has been developed to discriminate between the modified base 2-methyladenosine (m2A) and the unmodified counterpart Adenosine (A).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Nucleic Acid": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 0.2 μg of ab211495 for 50 μL Dynabeads® sheep-anti-rabbit IgG.", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.005-4 µg/mL", "ELISA-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

2-methyladenosine (m2A) is a modified nucleoside that plays a significant role in RNA structures impacting RNA stability and function. It is an adenosine derivative where a methyl group is added to the nitrogen at position 2 of the adenine ring altering its chemical properties. This modification has a relatively small molecular mass of approximately 281.3 Da. m2A is found primarily in tRNA and rRNA molecules within the cell contributing to their proper folding and stability. It is ubiquitously expressed across various tissues reflecting its broad functional significance.

Biological function summary

The modification of RNA by 2-methyladenosine contributes significantly to RNA processing and function. It plays a role in the structural integrity and proper folding of tRNA and rRNA by maintaining the stability of the RNA molecules they modify. m2A acts within ribonucleoprotein complexes where it influences the recognition and interaction between RNA and protein components. This modification ensures the accuracy of translation by maintaining the correct conformation of the RNA molecules involved in protein synthesis.

Pathways

2-methyladenosine engages in essential biological processes such as translation and RNA processing. It is involved in the ribosome biogenesis pathway which is important for protein synthesis in cells. Within this pathway m2A interacts with other RNA modifications and proteins like the ribosomal proteins and various RNA-binding proteins that guide RNA through the maturation process. The presence of m2A aids the efficient assembly of ribosomal units necessary for the synthesis of proteins.

Altered levels of 2-methyladenosine have been implicated in disorders related to dysfunction in protein synthesis. Aberrant m2A modification has been linked to certain cancers wherein improper RNA processing might contribute to uncontrolled cellular proliferation. Additionally it relates to neurodegenerative diseases where misfolded proteins result from translation dysregulation. In this context m2A modifications can affect the function of proteins such as ribosome-associated proteins and those involved in RNA methylation influencing disease outcomes through changes in RNA stability and processing.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information 2-methyladenosine (m2A)

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurodegeneration 20:103 PubMed41035073

2025

Molecular hallmarks of excitatory and inhibitory neuronal resilience to Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel Castanho,Pourya Naderi Yeganeh,Carles A Boix,Sarah L Morgan,Hansruedi Mathys,Dmitry Prokopenko,Bartholomew White,Larisa M Soto,Giulia Pegoraro,Saloni Shah,Athanasios Ploumakis,Nikolas Kalavros,David A Bennett,Christoph Lange,Doo Yeon Kim,Lars Bertram,Li-Huei Tsai,Manolis Kellis,Rudolph E Tanzi,Winston Hide

View all publications

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Abcam product promise

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保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-2-methyladenosine (m2A) 抗体 [EPR -19851-59]

Anti-2-methyladenosine (m2A) antibody [EPR -19851-59]

ELISA - Anti-2-methyladenosine (m2A) antibody [EPR -19851-59] (AB211495)

Immunoprecipitation - Anti-2-methyladenosine (m2A) antibody [EPR -19851-59] (AB211495)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise