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AB7794

Anti-160 kD Neurofilament Medium 抗体 [NF-09] - Neuronal Marker

Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker

4

(5 Reviews)

|

(50 Publications)

Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (ab7794) is a mouse monoclonal antibody detecting 160 kD Neurofilament Medium in Western Blot, IHC-P, IHC-Fr, ICC/IF, ELISA. Suitable for Cat, Cow, Human, Mouse, Pig, Rat.

- KO validated for confirmed specificity
- Over 50 publications
- Trusted since 2002

別名を表示する

NEF3, NFM, NEFM, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein

6 Images
Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)
  • WB

Lab

Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

Lanes 1 - 4 : Merged signal (red and green). Green - ab7794 observed at 150 kDa. Red - loading control, ab52866, observed at 50 kDa.

ab7794 was shown to specifically react with NEFM (Neurofilament) in wild-type HEK-293 cells as signal was lost in NEFM knockout cells. Wild-type and NEFM knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. ab7794 and ab52866 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (ab7794) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 whole cell lysate at 20 µg

Lane 2:

NEFM knockout HEK-293 whole cell lysate at 20 µg

Lane 3:

A549 whole cell lysate at 20 µg

Predicted band size: 102 kDa

Observed band size: 150 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)
  • ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

Immunofluorescence analysis of 1 month neuronal-differentiated dental pulp stem cells, staining 160 kD Neurofilament Medium with ab7794.

Cells were fixed with paraformaldehyde and incubated with primary antibody (1/600). A FITC-conjugated anti-mouse IgG (1/375) was used as the secondary antibody.

Image from Ferro F et al., PLoS One. 2012;7(7):e41774. Epub 2012 Jul 23. Fig 3.; doi:10.1371/journal.pone.0041774; July 23, 2012, PLoS ONE 7(7): e41774.

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

ab7794 staining Neurofilament medium protein in mouse Neuro2A cells by ICC/IF.

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

ICC/IF image of ab7794 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7794, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)
  • WB

Supplier Data

Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

Western blotting analysis of neurofilament medium protein in porcine brain lysate (reducing conditions) by mouse monoclonal NF-09.

All lanes:

Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (ab7794)

Predicted band size: 102 kDa

false

Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)
  • WB

Unknown

Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

Lanes 1-3 : Merged signal (red and green). Green - ab7794 observed at 150 kDa. Red - loading control ab181602 observed at 36 kDa.

ab7794 Anti-160 kD Neurofilament Medium antibody [NF-09] was shown to specifically react with 160 kD Neurofilament Medium in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266741 (knockout cell lysate ab257103) was used. Wild-type and 160 kD Neurofilament Medium knockout samples were subjected to SDS-PAGE. ab7794 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (ab7794) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

NEFM knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

Western blot - Human NEFM (160 kD Neurofilament Medium) knockout HEK-293T cell line (<a href='/products/cell-lines/human-nefm-160-kd-neurofilament-medium-knockout-hek-293t-cell-line-ab266741'>ab266741</a>)

Lane 3:

A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-680rd-preadsorbed-ab216777'>ab216777</a>) at 1/10000 dilution

Predicted band size: 102 kDa

Observed band size: 150 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

NF-09

アイソタイプ

IgG2a

軽鎖のタイプ

unknown

キャリアフリー

No

交差種

Mouse, Rat, Human, Pig, Cow, Cat

アプリケーション

ELISA, IHC-Fr, ICC/IF, IHC-P, IHC (PFA fixed), WB

applications

特異性

This antibody reacts with both phosphorylated and non-phosphorylated forms of medium neurofilament protein (160 kDa) of various species.

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Reactivity data

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"ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "<p></p>" }, "Cat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "<p></p>" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "<p></p>" }, "Mammals": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCPFAfixed-species-checked": "predicted", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" }, "Pig": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "<p></p>" } } }
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製品の詳細

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The 160 kD Neurofilament Medium also known as NEFM NF-M or NEF-M belongs to the family of neurofilament proteins. This protein is an important structural component of neurons particularly in the axonal cytoskeleton. Neurofilament Medium has a molecular mass of around 160 kilodaltons (kD). It shows high levels of expression in neuronal tissues contributing to the maintenance of axonal caliber. Commonly research recognizes it alongside other neurofilament proteins emphasizing its role in providing structural integrity to neurons.
Biological function summary

Neurofilament Medium participates in the formation of a stable network of neurofilaments within neurons. Acting as a part of the intermediate filament protein family it forms a complex with other neurofilaments such as NF-L and NF-H. This complex supports neuron structure and plays an important role in axonal transport. The heteropolymeric nature of neurofilaments contributes to their mechanical stability facilitating essential neuronal functions by aligning neurofilaments along the axon.

Pathways

Neurofilament Medium integrates into the cytoskeletal arrangement pathways that govern axonal transport and stability. It plays a significant role in the neuronal transport pathway associating with other proteins such as dynein and kinesin responsible for the motor functions along axons. Also it relates to the MAP kinase pathway where phosphorylation events modulate its assembly and disassembly dynamics in response to cellular signals.

Neurofilament Medium closely relates to neurodegenerative conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Aberrant phosphorylation or aggregation of NEFM can disrupt neuronal function leading to pathology. In ALS the abnormal accumulation of neurofilaments correlates with motor neuron degeneration. In Alzheimer's its interaction with tau protein poses significant interest as altered states of either could exacerbate neurofibrillary tangles worsening cognitive decline.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
See full target information NEFM
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文献 (50)

Recent publications for all applications. Explore the full list and refine your search

Biology 12: PubMed37372046

2023

Brain-Derived Estrogen Regulates Neurogenesis, Learning and Memory with Aging in Female Rats.

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Yuanyuan Huang,Wuxiang Sun,Fujia Gao,Haoran Ma,Tao Yuan,Zixuan Liu,Huiyu Liu,Jiewei Hu,Jing Bai,Xin Zhang,Ruimin Wang

Developmental dynamics : an official publication of the American Association of Anatomists 251:1834-1847 PubMed35727300

2022

Adhesion molecule Amigo2 is involved in the fasciculation process of the fasciculus retroflexus.

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Verónica Company,Raquel Murcia-Ramón,Abraham Andreu-Cervera,Paula Aracil-Pastor,Francisca Almagro-García,Salvador Martínez,Diego Echevarría,Eduardo Puelles

The Korean journal of pain 35:160-172 PubMed35354679

2022

Insulin enhances neurite extension and myelination of diabetic neuropathy neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Vuong M Pham,Nitish Thakor

Journal of experimental & clinical cancer research : CR 41:48 PubMed35109895

2022

Autophagic Schwann cells promote perineural invasion mediated by the NGF/ATG7 paracrine pathway in pancreatic cancer.

Applications

Unspecified application

Species

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Wunai Zhang,Rui He,Wenbin Yang,Yan Zhang,Qinggong Yuan,Jixin Wang,Yang Liu,Shuo Chen,Simei Zhang,Weifan Zhang,Zeen Zhu,Jing Zhang,Zheng Wang,Junhui Li

Frontiers in oncology 11:740410 PubMed34926255

2021

Reinvestigating Tumor-Ventricle Relationship of Craniopharyngiomas With Predominantly Ventricular Involvement: An Endoscopic Endonasal Series Based on Histopathological Assessment.

Applications

Unspecified application

Species

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Jun Fan,Yi Liu,Chaohu Wang,Zhanpeng Feng,Jun Pan,Yuping Peng,Junxiang Peng,Yun Bao,Jing Nie,Binghui Qiu,Songtao Qi

Nature communications 12:6839 PubMed34824237

2021

Cell-fate transition and determination analysis of mouse male germ cells throughout development.

Applications

Unspecified application

Species

Unspecified reactive species

Jiexiang Zhao,Ping Lu,Cong Wan,Yaping Huang,Manman Cui,Xinyan Yang,Yuqiong Hu,Yi Zheng,Ji Dong,Mei Wang,Shu Zhang,Zhaoting Liu,Shuhui Bian,Xiaoman Wang,Rui Wang,Shaofang Ren,Dazhuang Wang,Zhaokai Yao,Gang Chang,Fuchou Tang,Xiao-Yang Zhao

Frontiers in cell and developmental biology 9:755729 PubMed34722541

2021

Role in the Development of the Habenula and the Fasciculus Retroflexus.

Applications

Unspecified application

Species

Unspecified reactive species

Verónica Company,Ana Moreno-Cerdá,Abraham Andreu-Cervera,Raquel Murcia-Ramón,Francisca Almagro-García,Diego Echevarría,Salvador Martínez,Eduardo Puelles

Stem cell research & therapy 12:169 PubMed33691791

2021

Transcriptional factor FoxM1-activated microRNA-335-3p maintains the self-renewal of neural stem cells by inhibiting p53 signaling pathway via Fmr1.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaoying Jia,Yan Cui,Zhigang Tan,Min Liu,Yugang Jiang

The journal of international advanced otology 17:46-51 PubMed33605221

2021

Mammalian Cochlear Hair Cell Imaging Using Optical Coherence Tomography (OCT): A Preliminary Study.

Applications

Unspecified application

Species

Unspecified reactive species

Sung-Won Choi,Jieun Kang,Seokhwan Lee,Se-Joon Oh,Hongki Kim,Soo-Keun Kong

Cell cycle (Georgetown, Tex.) 19:3231-3248 PubMed33198579

2020

Overexpression of microRNA-221 promotes the differentiation of stem cells from human exfoliated deciduous teeth to neurons through activation of Wnt/β-catenin pathway via inhibition of CHD8.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Wen,Chenjiang He,Qin Zhang,Fanglin Zhang,Na Li,Yan Pan,Mengting Deng,Yue Wang,Jianping Li,Jiaxuan Qiu
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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