AB7794

Anti-160 kD Neurofilament Medium 抗体 [NF-09] - Neuronal Marker

Name: Anti-160 kD Neurofilament Medium 抗体 [NF-09] - Neuronal Marker (ab7794)
Brand: Abcam Limited
SKU: AB7794
Price: 56000 JPY
Availability: InStock
Rating: 4 (5 reviews)

Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker

KO Validated
Lab Essentials
詳細を見る

(5 Reviews)

(67 Publications)

Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (ab7794) is a mouse monoclonal antibody detecting 160 kD Neurofilament Medium in Western Blot, IHC-P, IHC-Fr, ICC/IF, ELISA. Suitable for Cat, Cow, Human, Mouse, Pig, Rat.

- KO validated for confirmed specificity
- Over 50 publications
- Trusted since 2002

別名を表示する

NEF3, NFM, NEFM, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein

6 Images

Lab

Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

Lanes 1 - 4 : Merged signal (red and green). Green - ab7794 observed at 150 kDa. Red - loading control, ab52866, observed at 50 kDa.

ab7794 was shown to specifically react with NEFM (Neurofilament) in wild-type HEK-293 cells as signal was lost in NEFM knockout cells. Wild-type and NEFM knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. ab7794 and ab52866 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (ab7794) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 whole cell lysate at 20 µg

Lane 2:

NEFM knockout HEK-293 whole cell lysate at 20 µg

Lane 3:

A549 whole cell lysate at 20 µg

Predicted band size: 102 kDa

Observed band size: 150 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

Immunofluorescence analysis of 1 month neuronal-differentiated dental pulp stem cells, staining 160 kD Neurofilament Medium with ab7794.

Cells were fixed with paraformaldehyde and incubated with primary antibody (1/600). A FITC-conjugated anti-mouse IgG (1/375) was used as the secondary antibody.

Image from Ferro F et al., PLoS One. 2012;7(7):e41774. Epub 2012 Jul 23. Fig 3.; doi:10.1371/journal.pone.0041774; July 23, 2012, PLoS ONE 7(7): e41774.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

ab7794 staining Neurofilament medium protein in mouse Neuro2A cells by ICC/IF.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

ICC/IF image of ab7794 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7794, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

Supplier Data

Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

Western blotting analysis of neurofilament medium protein in porcine brain lysate (reducing conditions) by mouse monoclonal NF-09.

All lanes:

Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (ab7794)

Predicted band size: 102 kDa

false

Unknown

Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

Lanes 1-3 : Merged signal (red and green). Green - ab7794 observed at 150 kDa. Red - loading control ab181602 observed at 36 kDa.

ab7794 Anti-160 kD Neurofilament Medium antibody [NF-09] was shown to specifically react with 160 kD Neurofilament Medium in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266741 (knockout cell lysate ab257103) was used. Wild-type and 160 kD Neurofilament Medium knockout samples were subjected to SDS-PAGE. ab7794 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (ab7794) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

NEFM knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

Western blot - Human NEFM (160 kD Neurofilament Medium) knockout HEK-293T cell line (<a href='/products/cell-lines/human-nefm-160-kd-neurofilament-medium-knockout-hek-293t-cell-line-ab266741'>ab266741</a>)

Lane 3:

A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-680rd-preadsorbed-ab216777'>ab216777</a>) at 1/10000 dilution

Predicted band size: 102 kDa

Observed band size: 150 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

NF-09

アイソタイプ

IgG2a

軽鎖のタイプ

unknown

キャリアフリー

交差種

Mouse, Rat, Human, Pig, Cow, Cat

アプリケーション

IHC (PFA fixed), IHC-P, WB, ICC/IF, IHC-Fr, ELISA

applications

特異性

This antibody reacts with both phosphorylated and non-phosphorylated forms of medium neurofilament protein (160 kDa) of various species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCPFAfixed" : {"fullname" : "Immunohistochemistry (PFA fixed)", "shortname":"IHC (PFA fixed)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" }, "Cat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" }, "Mammals": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCPFAfixed-species-checked": "predicted", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" }, "Pig": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (ab7794) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Cat, Cow, Human, Mouse, Pig, Rat samples.

Trusted by the scientific community
Anti-160 kD Neurofilament Medium [NF-09] - Neuronal Marker (ab7794) was first used in a scientific publication in 2002 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists
Anti-160 kD Neurofilament Medium [NF-09] - Neuronal Marker (ab7794) has over 5 independent reviews from customers.

Specificity confirmed
The specificity of Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (ab7794) has been confirmed by Western blot testing in NEFM Knockout HEK-293 cells.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The 160 kD Neurofilament Medium also known as NEFM NF-M or NEF-M belongs to the family of neurofilament proteins. This protein is an important structural component of neurons particularly in the axonal cytoskeleton. Neurofilament Medium has a molecular mass of around 160 kilodaltons (kD). It shows high levels of expression in neuronal tissues contributing to the maintenance of axonal caliber. Commonly research recognizes it alongside other neurofilament proteins emphasizing its role in providing structural integrity to neurons.

Biological function summary

Neurofilament Medium participates in the formation of a stable network of neurofilaments within neurons. Acting as a part of the intermediate filament protein family it forms a complex with other neurofilaments such as NF-L and NF-H. This complex supports neuron structure and plays an important role in axonal transport. The heteropolymeric nature of neurofilaments contributes to their mechanical stability facilitating essential neuronal functions by aligning neurofilaments along the axon.

Pathways

Neurofilament Medium integrates into the cytoskeletal arrangement pathways that govern axonal transport and stability. It plays a significant role in the neuronal transport pathway associating with other proteins such as dynein and kinesin responsible for the motor functions along axons. Also it relates to the MAP kinase pathway where phosphorylation events modulate its assembly and disassembly dynamics in response to cellular signals.

Neurofilament Medium closely relates to neurodegenerative conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Aberrant phosphorylation or aggregation of NEFM can disrupt neuronal function leading to pathology. In ALS the abnormal accumulation of neurofilaments correlates with motor neuron degeneration. In Alzheimer's its interaction with tau protein poses significant interest as altered states of either could exacerbate neurofibrillary tangles worsening cognitive decline.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

See full target information NEFM

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文献 (67)

Recent publications for all applications. Explore the full list and refine your search

Nature 646:1232-1242 PubMed40931074

2025

Neuronal activity-dependent mechanisms of small cell lung cancer pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Solomiia Savchuk,Kaylee M Gentry,Wengang Wang,Elana Carleton,Carlos A O Biagi-Junior,Karan Luthria,Belgin Yalçın,Lijun Ni,Hannah C Farnsworth,Rachel A Davis,Richard Drexler,Johannes C Melms,Yin Liu,Lehi Acosta-Alvarez,Griffin G Hartmann,Elisa C Pavarino,Jenna LaBelle,Pamelyn J Woo,Angus M Toland,Fangfei Qu,Yoon Seok Kim,Mariella G Filbin,Mark A Krasnow,Keith L Ligon,Benjamin Izar,Julien Sage,Bernardo L Sabatini,Michelle Monje,Humsa S Venkatesh

Nature neuroscience 28:1146-1159 PubMed40442499

2025

The transcriptomes, connections and development of submucosal neuron classes in the mouse small intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Khomgrit Morarach,Ziwei Liu,Sanghita Banerjee,Yanan Chen,Ashley L Harb,Joel M Kosareff,Charles R Hall,Fernando López-Redondo,Elham Jalalvand,Suad H Mohamed,Anastassia Mikhailova,David R Linden,Ulrika Marklund

Journal of neurochemistry 169:e70023 PubMed40066701

2025

Proteolysis-Based Biomarker Repertoire of the Neurofilament Proteome.

Applications

Unspecified application

Species

Unspecified reactive species

Axel Petzold

Communications biology 7:1713 PubMed39738805

2025

Abnormal cytoskeletal remodeling but normal neuronal excitability in a mouse model of the recurrent developmental and epileptic encephalopathy-susceptibility KCNB1-p.R312H variant.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandro Bortolami,Elena Forzisi Kathera-Ibarra,Anastasia Balatsky,Mansi Dubey,Rusheel Amin,Srinidi Venkateswaran,Stefania Dutto,Ishan Seth,Adam Ashor,Angel Nwandiko,Ping-Yue Pan,David P Crockett,Federico Sesti

Signal transduction and targeted therapy 9:254 PubMed39327467

2024

Palmitoylation regulates myelination by modulating the ZDHHC3-Cadm4 axis in the central nervous system.

Applications

Unspecified application

Species

Unspecified reactive species

Yanli Chang,Jiangli Zhu,Xiaopeng Li,Yi Deng,Birou Lai,Yidan Ma,Jia Tong,Huicong Liu,Juanjuan Li,Chenyu Yang,Qiao Chen,Chengbiao Lu,Yinming Liang,Shiqian Qi,Xiaoning Wang,Eryan Kong

Micromachines 15: PubMed39064373

2024

High-Porosity Sieve-Type Neural Electrodes for Motor Function Recovery and Nerve Signal Acquisition.

Applications

Unspecified application

Species

Unspecified reactive species

Wonsuk Choi,HyungDal Park,Seonghwan Oh,Seonho Seok,Dae Sung Yoon,Jinseok Kim

Journal of cellular and molecular medicine 28:e18122 PubMed38652110

2024

Proteomic studies in VWA1-related neuromyopathy allowed new pathophysiological insights and the definition of blood biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammed Athamneh,Nassam Daya,Andreas Hentschel,Andrea Gangfuss,Tobias Ruck,Adela Della Marina,Ulrike Schara-Schmidt,Albert Sickmann,Anne-Katrin Güttsches,Marcus Deschauer,Corinna Preusse,Matthias Vorgerd,Andreas Roos

Science translational medicine 16:eadf4601 PubMed38446899

2024

Gamma entrainment using audiovisual stimuli alleviates chemobrain pathology and cognitive impairment induced by chemotherapy in mice.

Applications

Unspecified application

Species

Unspecified reactive species

TaeHyun Kim,Benjamin T James,Martin C Kahn,Cristina Blanco-Duque,Fatema Abdurrob,Md Rezaul Islam,Nicolas S Lavoie,Manolis Kellis,Li-Huei Tsai

Frontiers in immunology 15:1347948 PubMed38370417

2024

IgLON5 deficiency produces behavioral alterations in a knockout mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Jon Landa,Ana Beatriz Serafim,Mercedes Alba,Estibaliz Maudes,Laura Molina-Porcel,Anna Garcia-Serra,Francesco Mannara,Josep Dalmau,Francesc Graus,Lidia Sabater

Nature communications 15:1377 PubMed38355941

2024

Elastic porous microspheres/extracellular matrix hydrogel injectable composites releasing dual bio-factors enable tissue regeneration.

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Yi Li,Siyang Liu,Jingjing Zhang,Yumeng Wang,Hongjiang Lu,Yuexi Zhang,Guangzhou Song,Fanhua Niu,Yufan Shen,Adam C Midgley,Wen Li,Deling Kong,Meifeng Zhu

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-160 kD Neurofilament Medium 抗体 [NF-09] - Neuronal Marker

Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker

Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

キャリアフリー

交差種

アプリケーション

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (67)

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