Anti-160 kD Neurofilament Medium 抗体 (ab9034)
Key features and details
- Rabbit polyclonal to 160 kD Neurofilament Medium
- Suitable for: IHC-FrFl, WB
- Reacts with: Mouse, Rat, Pig
- Isotype: IgG
製品の概要
-
製品名
Anti-160 kD Neurofilament Medium antibody
160 kD Neurofilament Medium 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to 160 kD Neurofilament Medium -
由来種
Rabbit -
特異性
Recognizes the evolutionarily conserved extreme C-terminal region of the medium neurofilament subunit (~145-170 kDa). -
アプリケーション
適用あり: IHC-FrFl, WBmore details -
種交差性
交差種: Mouse, Rat, Pig
交差が予測される動物種: Cat, Bird, Reptile
-
免疫原
Recombinant fragment corresponding to Rat 160 kD Neurofilament Medium (C terminal).
Database link: P12839 -
ポジティブ･コントロール
- IHC-FrFl: Rat cerebellum WB: Mouse, Rat and Pig brain and spinal cord tissue lysates
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9034の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-FrFl |
1/2000.
|
|
| WB |
1/5000.
|
| 特記事項 |
|---|
|
IHC-FrFl
1/2000. |
|
WB
1/5000. |
ターゲット情報
-
機能
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. -
配列類似性
Belongs to the intermediate filament family. -
翻訳後修飾
There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. - Information by UniProt
-
参照データベース
- Entrez Gene: 18040 Mouse
- Entrez Gene: 24588 Rat
- SwissProt: P08553 Mouse
- SwissProt: P12839 Rat
- Unigene: 242832 Mouse
- Unigene: 390700 Mouse
- Unigene: 10971 Rat
-
別名
- 150kDa medium antibody
- 160 kDa neurofilament protein antibody
- NEF3 antibody
see all
画像
-
Immunohistochemistry - Free Floating - Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034)Immunohistochemistry - Free Floating analysis of rat cerebellum section labeling 160 kD Neurofilament Medium with ab9034 at 1/2000 diution (red) and and costained with mouse mAb to GAP43 at 1/2000 diution (green). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with the above antibodies. ab9034 strongly labels neuronal processes throughout the cerebellum, while the GAP43 antibody stains predominantly synaptic regions in the molecular layer.
-
Western blot - Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034)All lanes : Anti-160 kD Neurofilament Medium antibody (ab9034) at 1/2000 dilution
Lane 1 : Protein standard
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat spinal cord tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Mouse spinal cord tissue lysate
Lane 6 : Pig brain tissue lysate
Lane 7 : Pig spinal cord tissue lysate
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (19)
ab9034 は 19 報の論文で使用されています。
- Shih NC et al. Microstructural mapping of dentate gyrus pathology in Alzheimer's disease: A 16.4 Tesla MRI study. Neuroimage Clin 37:103318 (2023). PubMed: 36630864
- Veličkov AI et al. Distributions of Platelet-Derived Growth Factor Receptor-α Positive Cells and Interstitial Cells of Cajal in the Colon of Rats with Diabetes Mellitus Type 2. Medicina (Kaunas) 59:N/A (2023). PubMed: 36837509
- Finszter CK et al. Early cellular and synaptic changes in dopaminoceptive forebrain regions of juvenile mice following gestational exposure to valproate. Front Neuroanat 17:1235047 (2023). PubMed: 37603782
- Colombo S et al. Stabilization of β-catenin promotes melanocyte specification at the expense of the Schwann cell lineage. Development 149:N/A (2022). PubMed: 34878101
- Mazzone SB et al. Transcriptional Profiling of Individual Airway Projecting Vagal Sensory Neurons. Mol Neurobiol 57:949-963 (2020). PubMed: 31630330