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リコンビナントRabMAb

Anti-15-PGDH 抗体 [EPR14332] (ab187160)

Reviews (2) Submit a question References (4)
Immunocytochemistry/ Immunofluorescence - Anti-15-PGDH antibody [EPR14332] (ab187160)
  • Immunocytochemistry/ Immunofluorescence - Anti-15-PGDH antibody [EPR14332] (ab187160)
  • Western blot - Anti-15-PGDH antibody [EPR14332] (ab187160)
  • Western blot - Anti-15-PGDH antibody [EPR14332] (ab187160)
  • Flow Cytometry (Intracellular) - Anti-15-PGDH antibody [EPR14332] (ab187160)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15-PGDH antibody [EPR14332] (ab187160)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15-PGDH antibody [EPR14332] (ab187160)
  • Anti-15-PGDH antibody [EPR14332] (ab187160)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14332] to 15-PGDH
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-15-PGDH antibody [EPR14332]
    15-PGDH 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14332] to 15-PGDH

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Cco-2, SW480, LoVo and Human placenta lysates; Human stomach and Human colon tissues; SW480 and Caco-2 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab187160の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/90.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/250.
WB (1)
1/1000 - 1/10000. Detects a band of approximately 25,29 kDa (predicted molecular weight: 29 kDa).
IHC-P (1)
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
1/90.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/250.
WB
1/1000 - 1/10000. Detects a band of approximately 25,29 kDa (predicted molecular weight: 29 kDa).
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Prostaglandin inactivation. Contributes to the regulation of events that are under the control of prostaglandin levels. Catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. Inhibits in vivo proliferation of colon cancer cells.

  • 組織特異性

    Detected in colon epithelium (at protein level).

  • 関連疾患

    Defects in HPGD are the cause of primary hypertrophic osteoathropathy autosomal recessive (PHOAR) [MIM:259100]; also known as pachydermoperiostosis autosomal recessive. Primary hypertrophic osteoarthropathy is characterized by digital clubbing, osterarthropathy, variable features of pachydermia, delayed closure of the fontanels, and congenital heart disease.
    Defects in HPGD are the cause of cranioosteoarthropathy (COA) [MIM:259100]. Clinical features include infantile onset of swelling of the joints, digital clubbing, hyperhidrosis, delayed closure of the fontanels, periostosis, and variable patent ductus arteriosus. Pachydermia is not a prominent feature.
    Defects in HPGD are a cause of isolated congenital nail clubbing (ICNC) [MIM:119900]; also called clubbing of digits or hereditary acropachy. ICNC is a rare genodermatosis characterized by enlargement of the nail plate and terminal segments of the fingers and toes, resulting from proliferation of the connective tissues between the nail matrix and the distal phalanx. It is usually symmetrical and bilateral (in some cases unilateral). In nail clubbing usually the distal end of the nail matrix is relatively high compared to the proximal end, while the nail plate is complete but its dimensions and diameter more or less vary in comparison to normal. There may be different fingers and toes involved to varying degrees. Some fingers or toes are spared, but the thumbs are almost always involved.

  • 配列類似性

    Belongs to the short-chain dehydrogenases/reductases (SDR) family.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 15 hydroxyprostaglandin dehydrogenase [NAD+] antibody
      • 15 PGDH antibody
      • 15-hydroxyprostaglandin dehydrogenase [NAD+] antibody
      • 15-PGDH antibody
      • 15PGDH antibody
      • Hpgd antibody
      • Hydroxyprostaglandin dehydrogenase 15 (NAD) antibody
      • NAD+ dependent 15 hydroxyprostaglandin dehydrogenase antibody
      • OTTHUMP00000218960 antibody
      • OTTHUMP00000219016 antibody
      • OTTHUMP00000219018 antibody
      • PGDH antibody
      • PGDH_HUMAN antibody
      • PGDH1 antibody
      • PHOAR1 antibody
      • Prostaglandin dehydrogenase 1 antibody
      • SDR36C1 antibody
      • Short chain dehydrogenase/reductase family 36C member 1 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-15-PGDH antibody [EPR14332] (ab187160)
      Immunocytochemistry/ Immunofluorescence - Anti-15-PGDH antibody [EPR14332] (ab187160)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast adenocarcinoma epithelial cell) labeling 15-PGDH with ab187160 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 was used as a counterstain (red).

      The nuclear counterstain is DAPI (blue).

      Confocal image showing mainly cytoplasmic staining in MCF-7 cell line.

    • Immunocytochemistry/ Immunofluorescence - Anti-15-PGDH antibody [EPR14332] (ab187160)
      Immunocytochemistry/ Immunofluorescence - Anti-15-PGDH antibody [EPR14332] (ab187160)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW480 (Human colorectal adenocarcinoma epithelial cell) labeling 15-PGDH with ab187160 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 was used as a counterstain (red).

      The nuclear counterstain is DAPI (blue).

      Confocal image showing mainly cytoplasmic staining in SW480 cell line.

    • Western blot - Anti-15-PGDH antibody [EPR14332] (ab187160)
      Western blot - Anti-15-PGDH antibody [EPR14332] (ab187160)
      All lanes : Anti-15-PGDH antibody [EPR14332] (ab187160) at 1/1000 dilution

      Lane 1 : HCT-15 (human colorectal carcinoma epithelial cell) whole cell lysate
      Lane 2 : HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate
      Lane 3 : PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate
      Lane 4 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate
      Lane 5 : Human colon tissue lysate
      Lane 6 : Human heart tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 29 kDa
      Observed band size: 29 kDa


      Exposure time: 80 seconds


      Blocking and Diluting Buffer: 5% NFDM/TBST

      The expression profile observed in HCT-116, PANC-1 and human heart are consistent with the literatures (PMID: 15542609, PMID: 30250298). Negative control: HCT-116, PANC-1 and human heart (PMID: 15542609, PMID: 30250298).

    • Western blot - Anti-15-PGDH antibody [EPR14332] (ab187160)
      Western blot - Anti-15-PGDH antibody [EPR14332] (ab187160)
      All lanes : Anti-15-PGDH antibody [EPR14332] (ab187160) at 1/10000 dilution

      Lane 1 : caco-2 cell lysate
      Lane 2 : SW480 cell lysate
      Lane 3 : LoVo cell lysate
      Lane 4 : Human placenta lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 29 kDa
      Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.

    • Flow Cytometry (Intracellular) - Anti-15-PGDH antibody [EPR14332] (ab187160)
      Flow Cytometry (Intracellular) - Anti-15-PGDH antibody [EPR14332] (ab187160)

      Intracellular flow cytometrical analysis of Caco-2 cells labeling 15-PGDH with ab187160 at 1/90 compared to a Isotype control. FITC-conjugated goat-anti-rabbit secondary antibody at 1/150 used for the analysis. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15-PGDH antibody [EPR14332] (ab187160)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15-PGDH antibody [EPR14332] (ab187160)

      Immunohistochemical analysis of paraffin embedded Human colon tissue labeling 15-PGDH with ab187160 at 1/4000 with a prediluted HRP Polymer for Rabbit IgG as secondary antibody.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15-PGDH antibody [EPR14332] (ab187160)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-15-PGDH antibody [EPR14332] (ab187160)

      Immunohistochemical analysis of paraffin embedded Human stomach tissue labeling 15-PGDH with ab187160 at 1/4000 with a prediluted HRP Polymer for Rabbit IgG as secondary antibody.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Anti-15-PGDH antibody [EPR14332] (ab187160)
      Anti-15-PGDH antibody [EPR14332] (ab187160)

    参考文献 (4)

    ab187160 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab187160 は 4 報の論文で使用されています。

    • He M  et al. Hpgd affects the progression of hypoxic pulmonary hypertension by regulating vascular remodeling. BMC Pulm Med 23:116 (2023). PubMed: 37055764
    • Yang F  et al. MiR-106b-5p regulates esophageal squamous cell carcinoma progression by binding to HPGD. BMC Cancer 22:308 (2022). PubMed: 35317779
    • Steenhaut P  et al. Changes in fetal membrane histology with cervical insufficiency and transabdominal cerclage. Int J Gynaecol Obstet 146:223-230 (2019). PubMed: 31004354
    • Wang W  et al. ROS-Mediated 15-Hydroxyprostaglandin Dehydrogenase Degradation via Cysteine Oxidation Promotes NAD+-Mediated Epithelial-Mesenchymal Transition. Cell Chem Biol 25:255-261.e4 (2018). PubMed: 29307841

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-15-PGDH antibody [EPR14332]

     Poor
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Ovarian Antral Follicle)
    Gel Running Conditions
    Reduced Denaturing (4–20% polyacrylamide gel)
    Loading amount
    30 µg
    Specification
    Ovarian Antral Follicle
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Dec 14 2022

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-15-PGDH antibody [EPR14332]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rhesus monkey Tissue sections (Placenta)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA
    Permeabilization
    No
    Specification
    Placenta
    Blocking step
    Vector Laboratories Supplier Diversity Partner VECTASTAIN Elite ABC HRP Kit as blocking agent for 30 minute(s) · Concentration: 10µg/mL
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Sudeshna Tripathy

    Verified customer

    投稿 Sep 26 2022

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