Anti-14-3-3 zeta (phospho S58) 抗体 (ab51109)
Key features and details
- Rabbit polyclonal to 14-3-3 zeta (phospho S58)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-14-3-3 zeta (phospho S58) antibody
14-3-3 zeta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to 14-3-3 zeta (phospho S58) -
由来種
Rabbit -
特異性
14-3-3 zeta (phospho S58) antibody detects endogenous levels of 14-3-3 zeta only when phosphorylated at serine 58. The immunogen sequence shows 92% homology with 14-3-3 gamma, eta, and beta/alpha. There may be cross reactivity with these other proteins. -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human 14-3-3 zeta aa 1-100 (phospho S58).
Database link: P63104 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51109の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
|
|
| WB | (2) |
1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
|
| IHC-P |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
|
WB
1/500 - 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. -
配列類似性
Belongs to the 14-3-3 family. -
翻訳後修飾
The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1. -
細胞内局在
Cytoplasm. Melanosome. Located to stage I to stage IV melanosomes. - Information by UniProt
-
参照データベース
- Entrez Gene: 7534 Human
- Entrez Gene: 22631 Mouse
- Omim: 601288 Human
- SwissProt: P63104 Human
- SwissProt: P63101 Mouse
- Unigene: 492407 Human
- Unigene: 3360 Mouse
- Unigene: 465895 Mouse
-
別名
- 14 3 3 delta antibody
- 14 3 3 protein zeta/delta antibody
- 14 3 3 protein/cytosolic phospholipase A2 antibody
see all
画像
-
Western blot - Anti-14-3-3 zeta (phospho S58) antibody (ab51109)All lanes : Anti-14-3-3 zeta (phospho S58) antibody (ab51109) at 1/500 dilution
Lane 1 : NIH/3T3 cell extract (treated with UV for 30 mins) with phosphopeptide immunogen (phospho S58)
Lane 2 : NIH/3T3 cell extract (treated with UV for 30 mins)
Predicted band size: 28 kDa
Observed band size: 28 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta (phospho S58) antibody (ab51109)Ab51109 staining human 14-3-3 zeta in human breast carcinoma tissue in immunohistochemistry using paraffin embedded tissue. -
Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 zeta (phospho S58) antibody (ab51109)ICC/IF image of ab51109 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51109, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (5)
ab51109 は 5 報の論文で使用されています。
- Kozeleková A et al. Phosphorylated and Phosphomimicking Variants May Differ-A Case Study of 14-3-3 Protein. Front Chem 10:835733 (2022). PubMed: 35321476
- Kaplan A et al. Small-Molecule Stabilization of 14-3-3 Protein-Protein Interactions Stimulates Axon Regeneration. Neuron 93:1082-1093.e5 (2017). PubMed: 28279353
- Lachaud C et al. 14-3-3-Regulated Ca(2+)-dependent protein kinase CPK3 is required for sphingolipid-induced cell death in Arabidopsis. Cell Death Differ : (2012). Arabidopsis thaliana . PubMed: 22935611
- Liu F et al. Shiga toxin (Stx)1B and Stx2B induce von Willebrand factor secretion from human umbilical vein endothelial cells through different signaling pathways. Blood 118:3392-8 (2011). WB ; Human . PubMed: 21816831
- Koseki N et al. 17ß-estradiol prevents reduction of retinal phosphorylated 14-3-3 zeta protein levels following a neurotoxic insult. Brain Res : (2011). WB ; Rat . PubMed: 22154405