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AB252662

Anti-14-3-3 zeta 抗体 [EPR20639-75] - BSA and Azide free (Detector)

Anti-14-3-3 zeta antibody [EPR20639-75] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal 1433Z antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

別名を表示する

14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ

1 Images
Sandwich ELISA - Anti-14-3-3 zeta antibody [EPR20639-75] - BSA and Azide free (Detector) (AB252662)
  • sELISA

Lab

Sandwich ELISA - Anti-14-3-3 zeta antibody [EPR20639-75] - BSA and Azide free (Detector) (AB252662)

Sandwich ELISA of ab253730 with the capture antibody ab252603 dilution at 2 µg/mL and detector antibody ab252662 dilution at 0.5 µg/mL.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR20639-75

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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組合せ製品

Sample Prep & Detection Kits

AB201796

Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link®

Conjugation - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link® (AB201796)

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  • 1
Sample Prep & Detection Kits

AB270705

5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20

Schematic Diagram - 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20 (AB270705)

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Primary Antibodies

AB309581

Anti-SMOC1 antibody [EPR26922-76] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-SMOC1 antibody [EPR26922-76] - BSA and Azide free (Detector) (AB309581)

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  • 0
Primary Antibodies

AB305131

Anti-PGK1 antibody [EPR24614-9] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-PGK1 antibody [EPR24614-9] - BSA and Azide free (Detector) (AB305131)

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  • 0
Primary Antibodies

AB237732

Anti-14-3-3 gamma/YWHAG antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 gamma/YWHAG antibody (AB237732)

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Assay Kits

AB253730

Human 14-3-3 zeta (YWHAZ) Antibody Pair - BSA and Azide free

RabMAb|Recombinant

Sandwich ELISA - Human 14-3-3 zeta (YWHAZ) Antibody Pair - BSA and Azide free (AB253730)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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製品の詳細

ab252662 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human 14-3-3 zeta (YWHAZ). The recommended pair for sandwich ELISA is:
Capture: ab252603, Human 14-3-3 zeta (YWHAZ) Capture Antibody (unconjugated)
Detector: ab252662, Human 14-3-3 zeta (YWHAZ) Detector Antibody (unconjugated)
The reference range value is 250 - 8000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The 14-3-3 zeta protein also known as 14-3-3 protein zeta/delta and 1433 protein belongs to the 14-3-3 protein family. These proteins have a molecular weight of around 28-33 kDa. 14-3-3 zeta proteins are expressed across various human tissues including the brain heart and muscles. Mechanically the 14-3-3 zeta protein interacts with different phosphorylated serine and threonine residues on its target proteins. This binding modulates the target proteins' conformation and activity influencing their function in cellular signaling and regulation.
Biological function summary

The 14-3-3 zeta protein plays an important role in several cellular processes. It acts as an adapter in signal transduction pathways and cell cycle control. Additionally the 14-3-3 zeta protein forms complexes with other proteins to regulate apoptosis and stress responses. By binding to various partners this protein influences the distribution and localization of proteins within the cell affecting diverse functions such as metabolism and cell growth.

Pathways

The 14-3-3 zeta protein participates actively in two primary pathways: the MAPK/ERK and PI3K/AKT pathways. Within the MAPK/ERK pathway it influences cellular differentiation and proliferation interacting closely with proteins like Raf and MEK. In the PI3K/AKT pathway 14-3-3 zeta affects cell survival working alongside proteins such as AKT and Bad. These interactions make 14-3-3 zeta a significant player in the regulation of cellular processes.

14-3-3 zeta protein has been connected with neurodegenerative diseases and cancer. Altered expression or function of this protein has been observed in conditions like Alzheimer's disease where it interacts with tau protein and in various cancers where 14-3-3 zeta impacts tumor progression by modulating the function of proteins like p53 and Rb. Understanding these interactions helps in exploring therapeutic strategies for disease intervention.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed : 14578935, PubMed : 15071501, PubMed : 15644438, PubMed : 16376338, PubMed : 16959763, PubMed : 31024343, PubMed : 9360956). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed : 35662396). Binding generally results in the modulation of the activity of the binding partner (PubMed : 35662396). Promotes cytosolic retention and inactivation of TFEB transcription factor by binding to phosphorylated TFEB (PubMed : 35662396). Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation (PubMed : 16959763). In neurons, regulates spine maturation through the modulation of ARHGEF7 activity (By similarity).
See full target information 14-3-3 protein zeta/delta
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Abcam product promise

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