Anti-14-3-3 zeta 抗体 (ab155037)
Key features and details
- Rabbit polyclonal to 14-3-3 zeta
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-14-3-3 zeta antibody
14-3-3 zeta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to 14-3-3 zeta -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Chicken, Cow
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免疫原
Recombinant fragment corresponding to a region within amino acids 1-229 of Human 14-3-3 zeta (UniProt P63104).
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ポジティブ･コントロール
- 293T, A431, H1299, HeLaS3, HepG2, Molt4, Raji and NIH 3T3 cell lysates; HeLaS3 cells; Human Cal27 xenograft tissue,
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab155037の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 28 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 28 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. -
配列類似性
Belongs to the 14-3-3 family. -
翻訳後修飾
The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1. -
細胞内局在
Cytoplasm. Melanosome. Located to stage I to stage IV melanosomes. - Information by UniProt
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参照データベース
- Entrez Gene: 425619 Chicken
- Entrez Gene: 287022 Cow
- Entrez Gene: 7534 Human
- Entrez Gene: 22631 Mouse
- Entrez Gene: 25578 Rat
- Omim: 601288 Human
- SwissProt: Q5ZKC9 Chicken
- SwissProt: P63103 Cow
see all -
別名
- 14 3 3 delta antibody
- 14 3 3 protein zeta/delta antibody
- 14 3 3 protein/cytosolic phospholipase A2 antibody
see all
画像
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Western blot - Anti-14-3-3 zeta antibody (ab155037)Anti-14-3-3 zeta antibody (ab155037) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 28 kDa
12% SDS PAGE -
Western blot - Anti-14-3-3 zeta antibody (ab155037)All lanes : Anti-14-3-3 zeta antibody (ab155037) at 1/2000 dilution
Lane 1 : Hep G2 whole cell lysate
Lane 2 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 28 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 zeta antibody (ab155037)Immunofluorescent analysis of paraformaldehyde-fixed HeLaS3 cells labeling 14-3-3 zeta with ab155037 at 1/100 dilution. Lower panel merged with DNA probe. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta antibody (ab155037)Immunohistochemical analysis of paraffin-embedded Cal27 xenograft tissue labeling 14-3-3 zeta with ab155037 at 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab155037 は 4 報の論文で使用されています。
- Yoon JH et al. Comparative Proteomic Analysis in Scar-Free Skin Regeneration in Acomys cahirinus and Scarring Mus musculus. Sci Rep 10:166 (2020). PubMed: 31932597
- Chen J et al. NLK interacts with 14-3-3? to restore the expression of E-cadherin. Oncol Rep 43:1845-1852 (2020). PubMed: 32236580
- Wang D et al. TRPC1 Deletion Causes Striatal Neuronal Cell Apoptosis and Proteomic Alterations in Mice. Front Aging Neurosci 10:72 (2018). PubMed: 29615894
- Liu P et al. The Isoquinoline Alkaloid Dauricine Targets Multiple Molecular Pathways to Ameliorate Alzheimer-Like Pathological Changes In Vitro. Oxid Med Cell Longev 2018:2025914 (2018). PubMed: 30057671