Anti-14-3-3 sigma/SFN 抗体 (ab77187)
Key features and details
- Goat polyclonal to 14-3-3 sigma/SFN
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-14-3-3 sigma/SFN antibody
14-3-3 sigma/SFN 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to 14-3-3 sigma/SFN -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Sheep, Cow, Dog, Pig, Zebrafish, Dictyostelium discoideum, Rainbow trout
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免疫原
Synthetic peptide corresponding to Human 14-3-3 sigma/SFN (internal sequence).
Sequence:DLHTLSEDSYKDST
Database link: P31947 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77187の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
1 hour primary incubation is recommended for this product. |
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| IHC-P |
Use a concentration of 2 - 4 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 2 - 4 µg/ml. |
ターゲット情報
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機能
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
p53-regulated inhibitor of G2/M progression. -
組織特異性
Present mainly in tissues enriched in stratified squamous keratinizing epithelium. -
配列類似性
Belongs to the 14-3-3 family. -
翻訳後修飾
Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation. -
細胞内局在
Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway. - Information by UniProt
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参照データベース
- Entrez Gene: 528453 Cow
- Entrez Gene: 487351 Dog
- Entrez Gene: 2810 Human
- Entrez Gene: 55948 Mouse
- Entrez Gene: 733625 Pig
- Entrez Gene: 313017 Rat
- Entrez Gene: 443032 Sheep
- Omim: 601290 Human
see all -
別名
- 14 3 3 protein sigma antibody
- 14-3-3 protein sigma antibody
- 1433S_HUMAN antibody
see all
画像
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Western blot - Anti-14-3-3 sigma/SFN antibody (ab77187)Anti-14-3-3 sigma/SFN antibody (ab77187) at 0.03 µg/ml + Human skin tissue lysate in RIPA buffer at 35 µg
Predicted band size: 28 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected using chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody (ab77187)Immunohistochemical analysis of paraffin embedded Human Skin tissue labeling 14-3-3 sigma/SFN with ab77187 at 2 ug/ml. Tissue underwent antigen retrieval in steam with Tris/EDTA buffer (pH 9.0). The HRP-staining procedure was used for detection.
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Western blot - Anti-14-3-3 sigma/SFN antibody (ab77187)All lanes : Anti-14-3-3 sigma/SFN antibody (ab77187) at 0.03 µg/ml
Lane 1 : A431 cell lysate in RIPA buffer
Lane 2 : U2OS cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 28 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected using chemiluminescence.
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Western blot - Anti-14-3-3 sigma/SFN antibody (ab77187)All lanes : Anti-14-3-3 sigma/SFN antibody (ab77187) at 0.03 µg/ml
Lane 1 : Mouse skin tissue lysate in RIPA buffer
Lane 2 : Rat skin tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 28 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected using chemiluminescence.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab77187 は 3 報の論文で使用されています。
- Mayr CH et al. Integrative analysis of cell state changes in lung fibrosis with peripheral protein biomarkers. EMBO Mol Med 13:e12871 (2021). PubMed: 33650774
- Kobayashi Y et al. Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis. Nat Cell Biol 22:934-946 (2020). PubMed: 32661339
- Plak K et al. Rap1-dependent pathways coordinate cytokinesis in Dictyostelium. Mol Biol Cell 25:4195-204 (2014). ICC/IF ; Dictyostelium discoideum . PubMed: 25298405