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AB193667

Anti-14-3-3 sigma/SFN 抗体 [EPR5873(2)]

Anti-14-3-3 sigma/SFN antibody [EPR5873(2)]

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(5 Publications)

Rabbit Recombinant Monoclonal 1433S antibody. Suitable for WB, IHC-P and reacts with Synthetic peptide - Human, Human, Synthetic peptide samples. Cited in 5 publications.

別名を表示する

HME1, SFN, 14-3-3 protein sigma, Epithelial cell marker protein 1, Stratifin

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling 14-3-3 sigma/SFN with ab193667 at 1/600 dilution (2μg/ml) followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)
  • WB

Supplier Data

Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)

Human full length 14-3-3 sigma/SFN recombinant protein is a house-made one with His-Tag.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667) at 1/1000 dilution

All lanes:

Human 14-3-3 sigma/SFN recombinant protein with His tag at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa

Observed band size: 28 kDa

false

Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)
  • WB

Supplier Data

Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667) at 1/1000 dilution

Lane 1:

Human skin lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Lane 3:

HACAT cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 27 kDa

Observed band size: 28 kDa

false

Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)
  • WB

Supplier Data

Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (ab193667) at 1/1000 dilution

Lane 1:

14-3-3 beta (YWHAB) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 2:

14-3-3 gamma (YWHAG) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 3:

14-3-3 epsilon (YWHAE) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 4:

14-3-3 zeta (YWHAZ) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 5:

14-3-3 eta (YWHAH) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 6:

14-3-3 theta (YWHAQ) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Lane 7:

14-3-3 sigma (SFN) Human Recombinant Protein 10 ng, with DDDK tagged at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 27 kDa

Observed band size: 28 kDa

false

Exposure time: 60s

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR5873(2)

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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代替製品

Primary Antibodies

AB151504

Anti-14-3-3 sigma/SFN antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody (AB151504)

  • 0
  • 0
Primary Antibodies

AB268067

Anti-14-3-3 sigma/SFN antibody [CPTC-SFN-2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [CPTC-SFN-2] (AB268067)

  • 0
  • 0
Primary Antibodies

AB86380

Anti-14-3-3 sigma/SFN antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody (AB86380)

  • 0
  • 0
Primary Antibodies

AB77187

Anti-14-3-3 sigma/SFN antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody (AB77187)

  • 0
  • 0

組合せ製品

Assay Kits

AB245711

Human SFN ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human SFN ELISA Kit (AB245711)

  • 0
  • 0
Primary Antibodies

AB109494

Anti-S100 alpha 2/S100A2 antibody [EPR5392]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 2/S100A2 antibody [EPR5392] (AB109494)

  • 0
  • 0
Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

  • 5
  • 19
Primary Antibodies

AB33899

Anti-BRAF antibody [EP152Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [EP152Y] (AB33899)

  • 4
  • 12
Primary Antibodies

AB109725

Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (AB109725)

  • 3
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/600", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Synthetic peptide": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/600", "IHCP-species-notes": "<p></p>" }, "Synthetic peptide - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Conditional Ambient
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The 14-3-3 sigma protein also known as SFN is a member of the 14-3-3 family of proteins. It plays a major role in cellular processes by regulating various signaling pathways. This protein has a molecular mass of about 28 kDa. The expression of 14-3-3 sigma is most notable in epithelial cells and it participates in cell cycle control. It interacts with a wide range of signaling molecules emphasizing its involvement in cellular regulation.
Biological function summary

14-3-3 sigma contributes to cell cycle arrest and apoptosis. As part of a complex it interacts with other proteins to ensure proper cell division and prevent abnormal growth. It binds to phosphorylated serine/threonine residues on its target proteins influencing their activity and function. By doing this it exerts control over cell cycle checkpoints and influences DNA damage response mechanisms.

Pathways

14-3-3 sigma interacts with several pathways that are critical for maintaining cell stability and response to stress. It participates in the PI3K/AKT pathway working closely with proteins like AKT1 to facilitate cell survival and growth suppression under stress conditions. Its involvement in this pathway ties it to the regulation of cell proliferation and survival interconnecting with broader cellular networks.

Disruptions in 14-3-3 sigma function are linked to cancer and neurodegenerative diseases. In cancer its downregulation or loss leads to unchecked cell division contributing to tumor progression. In neurodegenerative diseases such as Alzheimer's the protein's interaction with tau phosphorylation processes affects disease progression. Furthermore it interacts with proteins like p53 emphasizing its role in tumor suppression and cellular stress responses.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed : 15731107, PubMed : 22634725, PubMed : 28202711, PubMed : 37797010). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed : 15731107, PubMed : 22634725, PubMed : 28202711, PubMed : 37797010). Binding generally results in the modulation of the activity of the binding partner (PubMed : 15731107, PubMed : 22634725, PubMed : 28202711, PubMed : 37797010). Promotes cytosolic retention of GBP1 GTPase by binding to phosphorylated GBP1, thereby inhibiting the innate immune response (PubMed : 37797010). Also acts as a TP53/p53-regulated inhibitor of G2/M progression (PubMed : 9659898). When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). Acts to maintain desmosome cell junction adhesion in epithelial cells via interacting with and sequestering PKP3 to the cytoplasm, thereby restricting its translocation to existing desmosome structures and therefore maintaining desmosome protein homeostasis (PubMed : 24124604). Also acts to facilitate PKP3 exchange at desmosome plaques, thereby maintaining keratinocyte intercellular adhesion (PubMed : 29678907). May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53 (PubMed : 18382127).
See full target information SFN
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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 40:140-151 PubMed39415627

2024

H3K27 Acetylation-Activated GLDC Accelerated the Advancement of Oral Squamous Cell Carcinoma by Suppressing the p53 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Xu,Qingfeng Xu,Haibing Yang

The Journal of cell biology 223: PubMed38625077

2024

Determinants of minor satellite RNA function in chromosome segregation in mouse embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yung-Li Chen,Alisha N Jones,Amy Crawford,Michael Sattler,Andreas Ettinger,Maria-Elena Torres-Padilla

International journal of genomics 2023:9731675 PubMed37587914

2023

Stratifin Promotes Hepatocellular Carcinoma Progression by Modulating the Wnt/-Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shan-Ping Ye,Hong-Xin Yu,Wei-Jie Lu,Jun-Fu Wang,Tai-Yuan Li,Jun Shi,Xiao-Ye Cheng

Pharmaceuticals (Basel, Switzerland) 15: PubMed35890172

2022

ZLM-7 Blocks Breast Cancer Progression by Inhibiting MDM2 via Upregulation of 14-3-3 Sigma.

Applications

Unspecified application

Species

Unspecified reactive species

Min Wen,Zi-Zheng Zou,Tiao Luo,Xuan Li,Su-You Liu,Ji-Jia Li,Zhi-Yong Luo

Journal of cellular and molecular medicine 25:3524-3536 PubMed33683826

2021

ARID1B/SUB1-activated lncRNA HOXA-AS2 drives the malignant behaviour of hepatoblastoma through regulation of HOXA3.

Applications

Unspecified application

Species

Unspecified reactive species

Gongbao Liu,Baihui Liu,Xiangqi Liu,Lulu Xie,Jiajun He,Jingjing Zhang,Rui Dong,Duan Ma,Kuiran Dong,Mujie Ye
View all publications
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