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AB86380

Anti-14-3-3 sigma/SFN 抗体

Anti-14-3-3 sigma/SFN antibody

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(1 Publication)

Rabbit Polyclonal 1433S antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SFN aa 150 to C-terminus.

別名を表示する

HME1, SFN, 14-3-3 protein sigma, Epithelial cell marker protein 1, Stratifin

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody (AB86380)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody (AB86380)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling 14-3-3 sigma with ab86380 at 1/1000 (1µg/ml). Detection : DAB.

Immunoprecipitation - Anti-14-3-3 sigma/SFN antibody (AB86380)
  • IP

Unknown

Immunoprecipitation - Anti-14-3-3 sigma/SFN antibody (AB86380)

Detection of 14-3-3 sigma/SFN by Western blot of Immunprecipitate.
ab86380, at 1 μg/ml, staining 14-3-3 sigma/SFN in HeLa whole cell lysate immunoprecipitated using ab86380 at 3 μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-14-3-3 sigma/SFN antibody (ab86380)

Predicted band size: 27 kDa

false

Western blot - Anti-14-3-3 sigma/SFN antibody (AB86380)
  • WB

Unknown

Western blot - Anti-14-3-3 sigma/SFN antibody (AB86380)

All lanes:

Western blot - Anti-14-3-3 sigma/SFN antibody (ab86380) at 0.1 µg/mL

Lane 1:

Hela whole cell lysate at 50 µg

Lane 2:

Hela whole cell lysate at 15 µg

Lane 3:

Hela whole cell lysate at 5 µg

Predicted band size: 27 kDa

true

Exposure time: 30s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, IHC-P, IP

applications

免疫原

Synthetic Peptide within Human SFN aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P31947

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代替製品

Primary Antibodies

AB151504

Anti-14-3-3 sigma/SFN antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody (AB151504)

  • 0
  • 0
Primary Antibodies

AB268067

Anti-14-3-3 sigma/SFN antibody [CPTC-SFN-2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [CPTC-SFN-2] (AB268067)

  • 0
  • 0
Primary Antibodies

AB77187

Anti-14-3-3 sigma/SFN antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody (AB77187)

  • 0
  • 0
Primary Antibodies

AB193667

Anti-14-3-3 sigma/SFN antibody [EPR5873(2)]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)

  • 0
  • 0

組合せ製品

Assay Kits

AB245711

Human SFN ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human SFN ELISA Kit (AB245711)

  • 0
  • 0
Primary Antibodies

AB33899

Anti-BRAF antibody [EP152Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody [EP152Y] (AB33899)

  • 4
  • 12
Primary Antibodies

AB225628

Anti-A-Raf antibody

Immunoprecipitation - Anti-A-Raf antibody (AB225628)

  • 0
  • 0
Primary Antibodies

AB224817

Anti-Raf1 antibody

Lab Essentials

Immunoprecipitation - Anti-Raf1 antibody (AB224817)

  • 0
  • 0
Primary Antibodies

AB317438

Anti-14-3-3 zeta antibody [EPR27044-19]

20ul selling size|Recombinant|RabMAb

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 zeta antibody [EPR27044-19] (AB317438)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The 14-3-3 sigma protein also known as SFN is a member of the 14-3-3 family of proteins. It plays a major role in cellular processes by regulating various signaling pathways. This protein has a molecular mass of about 28 kDa. The expression of 14-3-3 sigma is most notable in epithelial cells and it participates in cell cycle control. It interacts with a wide range of signaling molecules emphasizing its involvement in cellular regulation.
Biological function summary

14-3-3 sigma contributes to cell cycle arrest and apoptosis. As part of a complex it interacts with other proteins to ensure proper cell division and prevent abnormal growth. It binds to phosphorylated serine/threonine residues on its target proteins influencing their activity and function. By doing this it exerts control over cell cycle checkpoints and influences DNA damage response mechanisms.

Pathways

14-3-3 sigma interacts with several pathways that are critical for maintaining cell stability and response to stress. It participates in the PI3K/AKT pathway working closely with proteins like AKT1 to facilitate cell survival and growth suppression under stress conditions. Its involvement in this pathway ties it to the regulation of cell proliferation and survival interconnecting with broader cellular networks.

Disruptions in 14-3-3 sigma function are linked to cancer and neurodegenerative diseases. In cancer its downregulation or loss leads to unchecked cell division contributing to tumor progression. In neurodegenerative diseases such as Alzheimer's the protein's interaction with tau phosphorylation processes affects disease progression. Furthermore it interacts with proteins like p53 emphasizing its role in tumor suppression and cellular stress responses.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed : 15731107, PubMed : 22634725, PubMed : 28202711, PubMed : 37797010). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed : 15731107, PubMed : 22634725, PubMed : 28202711, PubMed : 37797010). Binding generally results in the modulation of the activity of the binding partner (PubMed : 15731107, PubMed : 22634725, PubMed : 28202711, PubMed : 37797010). Promotes cytosolic retention of GBP1 GTPase by binding to phosphorylated GBP1, thereby inhibiting the innate immune response (PubMed : 37797010). Also acts as a TP53/p53-regulated inhibitor of G2/M progression (PubMed : 9659898). When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). Acts to maintain desmosome cell junction adhesion in epithelial cells via interacting with and sequestering PKP3 to the cytoplasm, thereby restricting its translocation to existing desmosome structures and therefore maintaining desmosome protein homeostasis (PubMed : 24124604). Also acts to facilitate PKP3 exchange at desmosome plaques, thereby maintaining keratinocyte intercellular adhesion (PubMed : 29678907). May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53 (PubMed : 18382127).
See full target information SFN
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer biomarkers : section A of Disease markers 38:287-300 PubMed37955079

2023

Secreted frizzled related-protein 2 is prognostic for human pancreatic cancer patient survival and is associated with fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Julie B Siegel,Patrick Nasarre,Lillian Hsu,Rupak Mukherjee,Meghan Gormley,Bailey Richardson,Imran Khan,Jordan E Morningstar,Eleanor Hilliard,John P O'Bryan,Kristi L Helke,Laura Spruill,Nathan G Dolloff,Nancy Klauber-DeMore
View all publications
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Abcam product promise

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