JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB77187

Anti-14-3-3 sigma/SFN 抗体

Anti-14-3-3 sigma/SFN antibody

Be the first to review this product! Submit a review

|

(3 Publications)

Goat Polyclonal 1433S antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SFN.

別名を表示する

HME1, SFN, 14-3-3 protein sigma, Epithelial cell marker protein 1, Stratifin

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody (AB77187)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody (AB77187)

Immunohistochemical analysis of paraffin embedded Human Skin tissue labeling 14-3-3 sigma/SFN with ab77187 at 2 ug/ml. Tissue underwent antigen retrieval in steam with Tris/EDTA buffer (pH 9.0). The HRP-staining procedure was used for detection.

Western blot - Anti-14-3-3 sigma/SFN antibody (AB77187)
  • WB

Supplier Data

Western blot - Anti-14-3-3 sigma/SFN antibody (AB77187)

Primary incubation was 1 hour. Detected using chemiluminescence.

All lanes:

Western blot - Anti-14-3-3 sigma/SFN antibody (ab77187) at 0.03 µg/mL

All lanes:

Human skin tissue lysate in RIPA buffer at 35 µg

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

Western blot - Anti-14-3-3 sigma/SFN antibody (AB77187)
  • WB

Supplier Data

Western blot - Anti-14-3-3 sigma/SFN antibody (AB77187)

Primary incubation was 1 hour. Detected using chemiluminescence.

All lanes:

Western blot - Anti-14-3-3 sigma/SFN antibody (ab77187) at 0.03 µg/mL

Lane 1:

A431 cell lysate in RIPA buffer at 35 µg

Lane 2:

U2OS cell lysate in RIPA buffer at 35 µg

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

Western blot - Anti-14-3-3 sigma/SFN antibody (AB77187)
  • WB

Supplier Data

Western blot - Anti-14-3-3 sigma/SFN antibody (AB77187)

Primary incubation was 1 hour. Detected using chemiluminescence.

All lanes:

Western blot - Anti-14-3-3 sigma/SFN antibody (ab77187) at 0.03 µg/mL

Lane 1:

Mouse skin tissue lysate in RIPA buffer at 35 µg

Lane 2:

Rat skin tissue lysate in RIPA buffer at 35 µg

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Human, Rat

アプリケーション

WB, IHC-P

applications

免疫原

Synthetic Peptide within Human SFN. The exact immunogen used to generate this antibody is proprietary information.

P31947

style
left-column

代替製品

Primary Antibodies

AB151504

Anti-14-3-3 sigma/SFN antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody (AB151504)

  • 0
  • 0
Primary Antibodies

AB86380

Anti-14-3-3 sigma/SFN antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody (AB86380)

  • 0
  • 0
Primary Antibodies

AB193667

Anti-14-3-3 sigma/SFN antibody [EPR5873(2)]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 sigma/SFN antibody [EPR5873(2)] (AB193667)

  • 0
  • 0

組合せ製品

Assay Kits

AB245711

Human SFN ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human SFN ELISA Kit (AB245711)

  • 0
  • 0
Primary Antibodies

AB109725

Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (AB109725)

  • 3
  • 1
Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

  • 5
  • 19
Primary Antibodies

AB268020

Anti-Fibronectin antibody [EPR23110-46]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR23110-46] (AB268020)

  • 4
  • 5
Primary Antibodies

AB138492

Anti-Collagen I antibody [EPR7785]

20ul selling size|BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [EPR7785] (AB138492)

  • 4
  • 37
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-4 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
バッファー組成
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The 14-3-3 sigma protein also known as SFN is a member of the 14-3-3 family of proteins. It plays a major role in cellular processes by regulating various signaling pathways. This protein has a molecular mass of about 28 kDa. The expression of 14-3-3 sigma is most notable in epithelial cells and it participates in cell cycle control. It interacts with a wide range of signaling molecules emphasizing its involvement in cellular regulation.
Biological function summary

14-3-3 sigma contributes to cell cycle arrest and apoptosis. As part of a complex it interacts with other proteins to ensure proper cell division and prevent abnormal growth. It binds to phosphorylated serine/threonine residues on its target proteins influencing their activity and function. By doing this it exerts control over cell cycle checkpoints and influences DNA damage response mechanisms.

Pathways

14-3-3 sigma interacts with several pathways that are critical for maintaining cell stability and response to stress. It participates in the PI3K/AKT pathway working closely with proteins like AKT1 to facilitate cell survival and growth suppression under stress conditions. Its involvement in this pathway ties it to the regulation of cell proliferation and survival interconnecting with broader cellular networks.

Disruptions in 14-3-3 sigma function are linked to cancer and neurodegenerative diseases. In cancer its downregulation or loss leads to unchecked cell division contributing to tumor progression. In neurodegenerative diseases such as Alzheimer's the protein's interaction with tau phosphorylation processes affects disease progression. Furthermore it interacts with proteins like p53 emphasizing its role in tumor suppression and cellular stress responses.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed : 15731107, PubMed : 22634725, PubMed : 28202711, PubMed : 37797010). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed : 15731107, PubMed : 22634725, PubMed : 28202711, PubMed : 37797010). Binding generally results in the modulation of the activity of the binding partner (PubMed : 15731107, PubMed : 22634725, PubMed : 28202711, PubMed : 37797010). Promotes cytosolic retention of GBP1 GTPase by binding to phosphorylated GBP1, thereby inhibiting the innate immune response (PubMed : 37797010). Also acts as a TP53/p53-regulated inhibitor of G2/M progression (PubMed : 9659898). When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). Acts to maintain desmosome cell junction adhesion in epithelial cells via interacting with and sequestering PKP3 to the cytoplasm, thereby restricting its translocation to existing desmosome structures and therefore maintaining desmosome protein homeostasis (PubMed : 24124604). Also acts to facilitate PKP3 exchange at desmosome plaques, thereby maintaining keratinocyte intercellular adhesion (PubMed : 29678907). May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53 (PubMed : 18382127).
See full target information SFN
style
left-column

文献 (3)

Recent publications for all applications. Explore the full list and refine your search

EMBO molecular medicine 13:e12871 PubMed33650774

2021

Integrative analysis of cell state changes in lung fibrosis with peripheral protein biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Christoph H Mayr,Lukas M Simon,Gabriela Leuschner,Meshal Ansari,Janine Schniering,Philipp E Geyer,Ilias Angelidis,Maximilian Strunz,Pawandeep Singh,Nikolaus Kneidinger,Frank Reichenberger,Edith Silbernagel,Stephan Böhm,Heiko Adler,Michael Lindner,Britta Maurer,Anne Hilgendorff,Antje Prasse,Jürgen Behr,Matthias Mann,Oliver Eickelberg,Fabian J Theis,Herbert B Schiller

Nature cell biology 22:934-946 PubMed32661339

2020

Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshihiko Kobayashi,Aleksandra Tata,Arvind Konkimalla,Hiroaki Katsura,Rebecca F Lee,Jianhong Ou,Nicholas E Banovich,Jonathan A Kropski,Purushothama Rao Tata

Molecular biology of the cell 25:4195-204 PubMed25298405

2014

Rap1-dependent pathways coordinate cytokinesis in Dictyostelium.

Applications

ICC/IF

Species

Dictyostelium discoideum

Katarzyna Plak,Ineke Keizer-Gunnink,Peter J M van Haastert,Arjan Kortholt
View all publications
style
left-column
style
left-column
style
right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com