Anti-14-3-3 (phospho S58) 抗体 (ab30554)
Key features and details
- Rabbit polyclonal to 14-3-3 (phospho S58)
- Suitable for: WB, IHC-Fr
- Reacts with: Mouse, Rat, Zebrafish
- Isotype: IgG
製品の概要
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製品名
Anti-14-3-3 (phospho S58) antibody
14-3-3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to 14-3-3 (phospho S58) -
由来種
Rabbit -
特異性
Specific for the ~29k 14-3-3 protein phosphorylated at Ser58. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
The N-terminal sequence used as antigen has high homology across a wide variety of species for all of the 14-3-3 proteins. We have not specifically tested it for reactivity against each protein in any given species, we do expect it to recognize most all of them.
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アプリケーション
適用あり: WB, IHC-Frmore details -
種交差性
交差種: Mouse, Rat, Zebrafish
交差が予測される動物種: Sheep, Chicken, Cow, Dog, Xenopus laevis, Non human primates
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免疫原
Synthetic peptide corresponding to 14-3-3 (phospho S58).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab30554の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 29 kDa.
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| IHC-Fr | (1) |
1/500.
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| 特記事項 |
|---|
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WB
1/1000. Detects a band of approximately 29 kDa. |
|
IHC-Fr
1/500. |
ターゲット情報
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機能
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. -
配列類似性
Belongs to the 14-3-3 family. -
翻訳後修飾
The alpha, brain-specific form differs from the beta form in being phosphorylated (By similarity). Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.
Isoform Short contains a N-acetylmethionine at position 1. -
細胞内局在
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 419190 Chicken
- Entrez Gene: 425619 Chicken
- Entrez Gene: 286863 Cow
- Entrez Gene: 287022 Cow
- Entrez Gene: 22631 Mouse
- Entrez Gene: 54401 Mouse
- Entrez Gene: 25578 Rat
- Entrez Gene: 56011 Rat
see all -
別名
- 14 3 3 antibody
- 14 3 3 protein beta antibody
- 14 3 3 protein beta/alpha antibody
see all
画像
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Western blot - Anti-14-3-3 (phospho S58) antibody (ab30554)All lanes : Anti-14-3-3 (phospho S58) antibody (ab30554) at 1/1000 dilution
Lane 1 : Rat brain stem lysate
Lane 2 : Rat brain stem lysate with the phosphopeptide used as the antigen
Observed band size: 29 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Frozen sections) - Anti-14-3-3 (phospho S58) antibody (ab30554)ab30554 staining 14-3-3 (phospho S58) in Mouse hippocampal tissue by Immunohistochemistry (Frozen sections). ab30554 is green, red staining is GFAP and nuclei are stained blue with DAPI. -
Western blot - Anti-14-3-3 (phospho S58) antibody (ab30554)All lanes : Anti-14-3-3 (phospho S58) antibody (ab30554) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate (20ug)
Lane 3 : Mouse brain homogenate (20ug)
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab30554 は 3 報の論文で使用されています。
- Cogo S et al. The Roc domain of LRRK2 as a hub for protein-protein interactions: a focus on PAK6 and its impact on RAB phosphorylation. Brain Res 1778:147781 (2022). PubMed: 35016853
- Civiero L et al. PAK6 Phosphorylates 14-3-3? to Regulate Steady State Phosphorylation of LRRK2. Front Mol Neurosci 10:417 (2017). PubMed: 29311810
- Okada N et al. A novel Chk1/2-Lats2-14-3-3 signaling pathway regulates P-body formation in response to UV damage. J Cell Sci 124:57-67 (2011). WB, ICC/IF ; Human . PubMed: 21118956