Anti-14-3-3 gamma/YWHAG 抗体 (ab155050)
Key features and details
- Rabbit polyclonal to 14-3-3 gamma/YWHAG
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-14-3-3 gamma/YWHAG antibody
14-3-3 gamma/YWHAG 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to 14-3-3 gamma/YWHAG -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Chicken, Cow, Monkey, Xenopus tropicalis
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免疫原
Recombinant fragment corresponding to Human 14-3-3 gamma/YWHAG aa 1-179.
Database link: P61981 -
ポジティブ･コントロール
- 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji and Mouse brain whole cell lysate; HeLa cells; Human DLD1 xenograft tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- HeLa whole cell lysate (ab29545)
- Raji whole cell lysate (ab30124)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab155050の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 28 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. -
組織特異性
Highly expressed in brain, skeletal muscle, and heart. -
配列類似性
Belongs to the 14-3-3 family. -
翻訳後修飾
Phosphorylated by various PKC isozymes. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 427820 Chicken
- Entrez Gene: 286862 Cow
- Entrez Gene: 7532 Human
- Entrez Gene: 22628 Mouse
- Entrez Gene: 56010 Rat
- Entrez Gene: 779762 Xenopus tropicalis
- Omim: 605356 Human
- SwissProt: Q5F3W6 Chicken
see all -
別名
- 14 3 3 gamma antibody
- 14 3 3 protein gamma antibody
- 14 3 3 protein gamma subtype antibody
see all
画像
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Western blot - Anti-14-3-3 gamma/YWHAG antibody (ab155050)All lanes : Anti-14-3-3 gamma/YWHAG antibody (ab155050) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : THP-1 cell lysate
Lane 3 : HL-60 cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 28 kDa -
Western blot - Anti-14-3-3 gamma/YWHAG antibody (ab155050)All lanes : Anti-14-3-3 gamma/YWHAG antibody (ab155050) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 28 kDa
12% SDS PAGE -
Western blot - Anti-14-3-3 gamma/YWHAG antibody (ab155050)Anti-14-3-3 gamma/YWHAG antibody (ab155050) at 1/10000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 28 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 gamma/YWHAG antibody (ab155050)Immunofluorescence analysis of methanol fixed HeLa cells labeling 14-3-3 gamma/YWHAG with ab155050 at 1/200 dilution. Lower image costained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 gamma/YWHAG antibody (ab155050)Immunohistochemical analysis of paraffin embedded Human DLD1 xenograft tissue labeling 14-3-3 gamma/YWHAG with ab155050 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab155050 は 4 報の論文で使用されています。
- Ni J et al. Functional genetic variants in centrosome-related genes CEP72 and YWHAG confer susceptibility to gastric cancer. Arch Toxicol 94:2861-2872 (2020). PubMed: 32535685
- Valdebenito S et al. Tunneling Nanotubes Mediate Adaptation of Glioblastoma Cells to Temozolomide and Ionizing Radiation Treatment. iScience 23:101450 (2020). PubMed: 32882515
- Cardona C et al. Identification of the iduronate-2-sulfatase proteome in wild-type mouse brain. Heliyon 5:e01667 (2019). PubMed: 31193135
- Chen XY et al. miR-140-5p mediates bevacizumab-induced cytotoxicity to cardiomyocytes by targeting the VEGFA/14-3-3? signal pathway. Toxicol Res (Camb) 8:875-884 (2019). PubMed: 32190292