Anti-14-3-3 beta 抗体 (ab15260)
Key features and details
- Rabbit polyclonal to 14-3-3 beta
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-14-3-3 beta antibody
14-3-3 beta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to 14-3-3 beta -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide within Human 14-3-3 beta aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P31946 -
特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab15260の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
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| WB | (3) |
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.
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| IHC-P |
1/100.
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| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa. |
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IHC-P
1/100. |
ターゲット情報
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機能
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. -
配列類似性
Belongs to the 14-3-3 family. -
翻訳後修飾
The alpha, brain-specific form differs from the beta form in being phosphorylated.
Isoform Short contains a N-acetylmethionine at position 1. -
細胞内局在
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 7529 Human
- Entrez Gene: 54401 Mouse
- Entrez Gene: 56011 Rat
- Omim: 601289 Human
- SwissProt: P31946 Human
- SwissProt: Q9CQV8 Mouse
- SwissProt: P35213 Rat
- Unigene: 643544 Human
see all -
別名
- 14 3 3 protein beta/alpha antibody
- 14-3-3 protein beta/alpha antibody
- 1433B_HUMAN antibody
see all
画像
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Western blot - Anti-14-3-3 beta antibody (ab15260)Anti-14-3-3 beta antibody (ab15260) at 1/25 dilution + HeLa cell lysate
Predicted band size: 28 kDa
Observed band size: 28 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 beta antibody (ab15260)ab15260 staining 14-3-3 beta in human brain by Immunohistochemistry (FFPE-sections).
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Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 beta antibody (ab15260)ICC/IF image of ab15260 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15260, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab15260 は 6 報の論文で使用されています。
- Yang Q et al. Regulation of lipolysis by 14-3-3 proteins on human adipocyte lipid droplets. PNAS Nexus 2:pgad420 (2023). PubMed: 38130664
- Gabizon R et al. A simple method for developing lysine targeted covalent protein reagents. Nat Commun 14:7933 (2023). PubMed: 38040731
- de Boni L et al. Brain region-specific susceptibility of Lewy body pathology in synucleinopathies is governed by α-synuclein conformations. Acta Neuropathol 143:453-469 (2022). PubMed: 35141810
- Iwahori S et al. Serine 13 of the human cytomegalovirus viral cyclin-dependent kinase UL97 is required for regulatory protein 14-3-3 binding and UL97 stability. J Biol Chem 298:102513 (2022). PubMed: 36150501
- Liu L et al. 14-3-3ß exerts glioma-promoting effects and is associated with malignant progression and poor prognosis in patients with glioma. Exp Ther Med 15:2381-2387 (2018). PubMed: 29467845
- Gawinecka J et al. Desmoplakin as a potential candidate for cerebrospinal fluid marker to rule out 14-3-3 false positive rates in sporadic Creutzfeldt-Jakob disease differential diagnosis. Neurodegener Dis 9:139-44 (2012). WB ; Human . PubMed: 22213780