AB208199

Anti-1-methylguanosine (m1G) 抗体 [EPR19833-150]

Anti-1-methylguanosine (m1G) antibody [EPR19833-150]

20ul selling size
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal 1-methylguanosine (m1G) antibody. Suitable for IP, ELISA, FRET, Dot and reacts with Chemical, Synthetic peptide samples.

別名を表示する

1 methylguanosine, m1G

5 Images

Supplier Data

Dot Blot - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

Dot blot analysis of 1-methylguanosine (m1G) using ab208199 at 1/500 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20,000 dilution (Left) or Methylene Blue (Right).

Lane 1 : HeLa total RNA

Lane 2 : Yeast tRNA

Exposure time : 8 seconds

ELISA - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

ELISA

Supplier Data

ELISA - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

BSA-conjugated m1G (modified) and G (unmodified) nucleosides were coated onto wells of a 96 well plate. ELISA was performed on 1.0 μg/ml of antigen using ab208199 at a concentration range of 0.005-4.000 μg/ml, followed by Goat Anti-Rabbit IgG, (H+L), alkaline phosphatase conjugated secondary antibody at 1/2500 dilution.

Supplier Data

Immunoprecipitation - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

The IP was performed in a U-bottom non-adsorbing propylene 96-well plate.

ab208199 (0.2 μg) was coated into Dynabeads^® sheep-anti-rabbit IgG (50 μl) for 1h at RT.

Unmodified/modified oligonucleotides (5 μM) were added to samples containing the antibody/bead complexes and incubated with agitation for 1 hour at RT.

After washing, Peroxidase-conjugated Streptavidin was incubated at 1/1000 dilution with agitation for 1 hour at RT.

ECL substrate was then added and the results read in a non-transparent 96-well plate with a digital detector and analyzed using ImageJ.

Lane 1 : Buffer only.

Lane 2 : Modified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[m1G]*.mN.mN.mN.mN.mN 3'

Lane 3 : Unmodified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[G]*.mN.mN.mN.mN.mN 3'

N - equimolar mixture of (A/U/G/C)
m - 2'O methyl protection
* - phosphorothioate protection

Blocking buffer and concentration : 5% NFDM/TBST

Dilution buffer and concentration : TBST/0.1% Triton X-100/1 mM EDTA

All lanes:

Immunoprecipitation - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (ab208199)

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FRET - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

FRET

Supplier Data

FRET - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

A FRET-based assay was performed using 25 nm of each biotinylated oligo using ab208199 at a concentration range of 0.001-4.000 µg/ml.

Supplier Data

Dot Blot - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

Lane 1 : Biotin-m1G RNA oligo

Lane 2 : Biotin-G RNA oligo

Lane 3 : Biotin-m2G RNA oligo

Lane 4 : Biotin-m22G RNA oligo

Exposure time 5 seconds

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR19833-150

アイソタイプ

IgG

キャリアフリー

アプリケーション

IP, FRET, Dot, ELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "FRET" : {"fullname" : "FRET", "shortname":"FRET"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.005-4 µg/mL", "ELISA-species-notes": "", "FRET-species-checked": "testedAndGuaranteed", "FRET-species-dilution-info": "0.001-4 µg/mL", "FRET-species-notes": "HTRF readout", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "FRET-species-checked": "notRecommended", "FRET-species-dilution-info": "", "FRET-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/500", "Dot-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

1-methylguanosine (m1G) is a modified nucleoside with a distinct function in RNA biology. It is a methylated derivative of guanosine. This target plays an important role in RNA modification and stability. m1G results from the methylation of guanosine at the first position and has a molecular mass of about 167.15 g/mol. Expression of m1G occurs in various types of RNA across eukaryotic and prokaryotic organisms showing its widespread significance in biological systems. Although m1G itself is not a protein its presence impacts the mechanical aspects of the RNA molecule where it resides.

Biological function summary

M1G impacts RNA functionality by influencing RNA folding and structural conformation. It forms integral parts of RNA molecules and contributes to their stability and function. m1G does not act in isolation but is part of a broader set of modified nucleosides that collectively enhance RNA function. The modifications involving m1G occur as part of post-transcriptional modification processes. These are often important for ensuring proper decoding and function of RNA during gene expression.

Pathways

Research indicates that the presence of m1G within RNA molecules can influence specific biological pathways related to translation and RNA stability. m1G impacts the efficiency and fidelity of translational machinery playing a part in the translation pathway. Additionally it interacts with various proteins like tRNA methyltransferases which facilitate its methylation in RNA. These enzymes are critical in pathways that ensure the correctness of RNA modifications which ultimately influence protein synthesis.

Researchers associate aberrant levels of m1G with certain conditions such as neurodegenerative diseases and cancer. Disruption in the normal modification process of guanosine to m1G can lead to dysfunctional RNA which relates to disease pathology. Disorders in m1G modification often implicate other proteins involved in RNA modification like small nucleolar RNAs (snoRNAs) which assist in inserting this modification. Understanding m1G's role in disease progression helps develop targeted therapeutic strategies focusing on the modulation of these RNA-modifying enzymes.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information 1-methylguanosine (m1G)

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-1-methylguanosine (m1G) 抗体 [EPR19833-150]

Anti-1-methylguanosine (m1G) antibody [EPR19833-150]

Dot Blot - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

ELISA - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

Immunoprecipitation - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

FRET - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

Dot Blot - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (AB208199)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise