Complex II WB 抗体 Cocktail (ab110410)
Key features and details
- Assay type: Quantitative
製品の概要
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製品名
Complex II WB Antibody Cocktail
Complex II キット 製品一覧 -
アッセイタイプ
Quantitative -
種交差性
交差種: Mouse, Rat, Cow, Human -
製品の概要
Contains a total of 305 µg of antibodies and is a pre-mixed solution of 3 monoclonal antibodies against Complex II subunits 70 kDa Fp, 30 kDa Ip along with an antibody against Complex V alpha as a control. This cocktail can be used to verify isolated Complex II purity or to conveniently determine the levels of 2 of the 4 Complex II subunits in cell lines relative to the control protein (Complex V alpha).
Cocktail Antibodies:
Mouse C-V-alpha [15H4C4] monoclonal:
Amount: 50 µg
Working concentration: 1 µg/ml
Mouse C-II-30 (FeS) [21A11AE7] monoclonal:
Amount: 250 µg
Working concentration: 5 µg/ml
Mouse C-II-70 (FL) [2E3GC12FB2AE2] monoclonal:
Amount: 5 µg
Working concentration: 0.1 µg/ml
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特記事項
Related products
Review the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
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アプリケーション
適用あり: WBmore details
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 305 µg Pre-mixed solution of 3 monoclonal antibodies 1 x 305µg -
研究分野
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関連性
Complex II is also called succinate ubiquinone oxidoreductase or more commonly succinate dehydrogenase complex. This complex is composed of four nuclear encoded subunits and contains a flavin (FAD), non-heme iron centers and a b-type cytochrome as prosthetic groups. It is both a component of the electron transport chain and an enzyme of the Krebs cycle. Complex II deficiencies are seen in OXPHOS genetic disease and found in a type of cancer called paraganglioma. -
別名
- SDH
- Succinate coenzyme Q reductase
- Succinate dehydrogenase
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参照データベース
- Entrez Gene: 6390 Human
- Entrez Gene: 6389 Human
- Entrez Gene: 6391 Human
- SwissProt: P31040 Human
- SwissProt: P21912 Human
- SwissProt: Q99643 Human
- SwissProt: O14521 Human
- SwissProt: 6392 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110410の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 6 µg/ml. The antibody cocktail (1.5 mg/mL) should be diluted 250x to a final working concentration of 6 µg/mL for Western blotting.
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| 特記事項 |
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WB
Use a concentration of 6 µg/ml. The antibody cocktail (1.5 mg/mL) should be diluted 250x to a final working concentration of 6 µg/mL for Western blotting. |
画像
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Western blot - Complex II WB Antibody Cocktail (ab110410)All lanes : Complex II WB Antibody Cocktail (ab110410) at 1/250 dilution
Lane 1 : human heart mitochondria at 10 µg
Lane 2 : Immunoprecipitated Complex II at 0.5 µgThe Western blot cocktail ab110410 contains two Complex II subunits SDHA (70 kDa) and SDHB (30 kDa) and a general mitochondrial marker ATP synthase alpha subunit (F1α, 55 kDa). All three are present in the mitochondrial sample (1). The Complex II sample purified by Complex II immunoprecipitation beads (ab109799) shows only Complex II subunits (2).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (33)
ab110410 は 33 報の論文で使用されています。
- Gundu C et al. GSK2606414 attenuates PERK/p-eIF2α/ATF4/CHOP axis and augments mitochondrial function to mitigate high glucose induced neurotoxicity in N2A cells. Curr Res Pharmacol Drug Discov 3:100087 (2022). PubMed: 35146419
- Li J et al. Energy Metabolic Disorder of Astrocytes May Be an Inducer of Migraine Attack. Brain Sci 12:N/A (2022). PubMed: 35884650
- Timmons GA et al. The Circadian Clock Protein BMAL1 Acts as a Metabolic Sensor In Macrophages to Control the Production of Pro IL-1ß. Front Immunol 12:700431 (2021). PubMed: 34858390
- Wu P et al. Ginsenoside Rg3 alleviates septic liver injury by regulating the lncRNA TUG1/miR-200c-3p/SIRT1 axis. J Inflamm (Lond) 18:31 (2021). PubMed: 34930287
- Wu K et al. Cigarette smoke extract increases mitochondrial membrane permeability through activation of adenine nucleotide translocator (ANT) in lung epithelial cells. Biochem Biophys Res Commun 525:733-739 (2020). PubMed: 32143825