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Mounting Medium for IHC - Aqueous (ab64230)

Submit a review Q&A (3)References (9)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mounting Medium (ab64230)

    製品の概要

    • 製品名

      Mounting Medium for IHC - Aqueous

    • アプリケーション

      適用あり: IHC-Pmore details

    • 特記事項

      Mounting Medium ab64230 is an aqueous mounting medium designed for use with chromogenic tissue and cell staining. It can be used with chromogens such as Fast-Red or AEC that require an aqueous mounting medium, or those that do not, such as DAB. When it is dry, it generates an even surface and can be used without a glass coverslip.

      Mounting media products

      For fluorescent cell and tissue staining, Abcam recommends aqueous, anti-fade Fluorescence Mounting Medium ab104135, Mounting Medium with DAPI ab104139, and Mounting Medium with PI ab104129. For thick sections or tissues containing lots of fat, we recommend Glycerol Mounting Medium with DAPI ab188804.

      For chromogenic immunohistochemistry, such as with DAB, AEC, or Fast Red, we recommend this product, Mounting Medium ab64230, or organic Limonene Mounting Medium ab104141. 

       

      IHC protocol suitable for use with Mounting Medium ab64230:

      1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.

      2. Use appropriate antigen retrieval buffer or enzyme (primary antibody dependent) to treat sections. Wash 3 times in buffer.

      3. Add enough hydrogen peroxide blocking solution to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, use avidin biotin blocking.

      4. Apply protein block (or normal serum from same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.

      5. Apply primary antibody in antibody diluent and incubate.

      6. Wash 4 times in buffer. Incubate slide with biotinylated secondary antibody (or HRP polymer secondary antibody and skip step 7). Wash 4 times in buffer.

      7. Apply streptavidin-HRP and incubate for 10 minutes at room temperature.

      8. Rinse 4 times in buffer. Place slide in DAB substrate or AEC Substrate and incubate until desired color is achieved (1-10 mins). Rinse 4 times in buffer.

      9. Add enough drops of hematoxylin to cover the section. Incubate for 1 minute.

      10. Rinse 7-8 times in tap water. Add Mounting Medium ab64230 to cover the section.


      Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Store at room temperature.

    • バッファー

      Preservative: 0.1% Sodium azide
    • Concentration information loading...

    • IHC 試薬 備考

      ab64230 is formulated for coverslipping sections. It can be used with chromogens such as Fast-Red or AEC that require an aqueous mounting medium, or those that do not, such as DAB. When it is dry, it generates an even surface and can be used without a glass coverslip.

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab64230の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P
    Use at an assay dependent dilution.
    特記事項
    IHC-P
    Use at an assay dependent dilution.

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mounting Medium (ab64230)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mounting Medium (ab64230)
      ab64230 Mounting Medium used along with anti-CD20 antibody on Human Tonsil

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (9)

    ab64230 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab64230 は 9 報の論文で使用されています。

    • Li M  et al. Long non-coding RNA AFAP1-AS1 facilitates the growth and invasiveness of oral squamous cell carcinoma by regulating the miR-145/HOXA1 axis. Oncol Rep 45:1094-1104 (2021). PubMed: 33650645
    • Tang T  et al. Pterostilbene reduces endothelial cell injury in vascular arterial walls by regulating the Nrf2-mediated AMPK/STAT3 pathway in an atherosclerosis rat model. Exp Ther Med 19:45-52 (2020). PubMed: 31853271
    • Tang Y  et al. Knockdown of eukaryotic translation initiation factor 5A2 enhances the therapeutic efficiency of doxorubicin in hepatocellular carcinoma cells by triggering lethal autophagy. Int J Oncol 57:1368-1380 (2020). PubMed: 33174013
    • Kole E  et al. Effects of melatonin on ovarian reserve in cigarette smoking: an experimental study. Arch Med Sci 16:1376-1386 (2020). PubMed: 33224337
    • Kim YS  et al. Novel Medicine for Endometriosis and Its Therapeutic Effect in a Mouse Model. Biomedicines 8:N/A (2020). PubMed: 33339236
    • Lu J  et al. Resveratrol alleviates inflammatory injury and enhances the apoptosis of fibroblast-like synoviocytes via mitochondrial dysfunction and ER stress in rats with adjuvant arthritis. Mol Med Rep 20:463-472 (2019). PubMed: 31180523
    • Liang Y  et al. Protective effect of extracorporeal membrane oxygenation on intestinal mucosal injury after cardiopulmonary resuscitation in pigs. Exp Ther Med 18:4347-4355 (2019). PubMed: 31777541
    • Chen YC  et al. Laser-emission imaging of nuclear biomarkers for high-contrast cancer screening and immunodiagnosis. Nat Biomed Eng 1:724-735 (2017). PubMed: 29204310
    • Nakamura F  et al. Documentation and localization of force-mediated filamin A domain perturbations in moving cells. Nat Commun 5:4656 (2014). PubMed: 25120197

    レビューと Q&A

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    1-3 of 3 Abreviews or Q&A

    Question

    xxxx;

    As I told you on the phone, I am planning on performing IHC on fresh PBMC samples from sheep.

    Cells will be isolated from whole blood using a density gradient and will be placed on glass slides using a cytocentrifuge. At this point, we CAN fix them if needed, but we don’t have to. What do you recommend?

    Our primary antibody is affinity purified rabbit polyclonal. We care considering using your EXPOSE Rabbit specific HRP-AES detection kit (ab94361) – do you think this is the best kit to use, or would you recommend a different one for our purposes? We are attempting to stain a transmembrane protein.

    We are planning to counterstain using hematoxylin: do you think we would get the best contrast with the AEC kit or would you recommend using DAB?

    Also, does abcam recommend purchasing hematoxylin from any particular vendor?

    If ab64230 the appropriate mounting medium to use with this kit?

    Thanks so much for your help. I look forward to your response!

    Read More
    Answer

    Thank you for replying with that information.

    As I mentioned over the phone, I have spoken to the source of these kits and we do not have any experimental data using either the ACE or DABdetection kits (ab64260, ab64261, ab80437 and ab94361)for IHC on fresh PBMC samples, asallof these kits have only been tested on formalin fixed paraffin embedded tissue samples.Wewould expect that as long as the primary antibody binds the target as expected that both kits could be suitable foryour samples but as we have not tested it ourselves we would not be able to guarentee this.



    There should be good contrast if using the AEC or DAB kit with hematoxylin as counterstain. We counterstain with hematoxylin in-house regardless ofwhether DAB or AEC is used. The sensitivity betweenAEC and DABis similar. I would recommend using ab94361 or ab80437 if you expect the PBMC samples to contain any endogenous biotin.I am sorry but I cannot recommend a particular supplier of hematoxylin.

    And to answer your final question, the mounting media ab64230 should be suitable for your purposes.

    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

    Read More

    Question

    Dear Madam / Sir, A customer would like to buy a mounting medium to use IHC and IF. Which ABCAM mounting medium can be used with both of these applications? Thank you in advance.

    Read More
    Answer

    Thank you for contacting us.

    Differences in the chemical compositions of the mounting medium make using one product for both IHC and IF difficult. The conditions required for excellent perioxidase preservation are not neccessarily good for the anti- photobleaching requirements of IF. As such I cannot recommend a single medium for you customer but suggest they by mounting mediums specific to their needs.

    Our standard mointing medium (ab64230;https://www.abcam.com/Mounting-Medium-ab64230.html) is appropriate for IHC.



    For IF I recommend BrightMount/Plus (ab103748;https://www.abcam.com/BrightMount-Plus-25ml-Aqueous-Mounting-Medium-Anti-fading-for-Fluorescent-Staining-ab103748.html)


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Dear Technical Team, Hope this e-mail finds you well! Kindly arrange to send us the certificate of compliance (CoC) for the below Abcam products as desired by one of our customers : Cat No. Description ab97021 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin) ab97049 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin) ab97023 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) ab60314 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Chromeo™ 488) ab97080 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed ab65623 ECL Western Blotting Substrate Kit - 50 Tests ab52476 Rabbit monoclonal [EP1829Y] to CaMKII ab11800 Goat polyclonal to DCXR ab7260 Rabbit polyclonal to GFAP - Astrocyte Marker ab104224 Mouse monoclonal [1B7] to FOX3 ab76260 Rabbit monoclonal [EP2162Y] to Synapsin I (phospho S9) ab64230 Mounting Medium ab82477 Rabbit polyclonal to Adenosine A1 Receptor ab3462 Rabbit polyclonal to alpha 1 Adrenergic Receptor

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    Answer

    I am beginning to gather the CoC's you have requested. When you have a chance, please provide the lot number from each catalog number so I can include this information on the documentation. Thanks for the additional information.

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