Mouse GSK3 beta 抗体 Pair - BSA and Azide free (ab243994)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Mouse
製品の概要
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製品名
Mouse GSK3 beta Antibody Pair - BSA and Azide free
GSK3 beta キット 製品一覧 -
アッセイタイプ
ELISA set -
検出範囲
468.75 pg/ml - 30000 pg/ml -
種交差性
交差種: Mouse -
製品の概要
The Antibody Pair can be used to quantify Mouse GSK3 beta. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab218264), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Reagents
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
キャリア・フリー
はい -
内容 10 x 96 tests Mouse GSK3 beta Capture Antibody (unconjugated) 1 x 100µg Mouse GSK3 beta Detector Antibody (unconjugated) 1 x 100µg -
研究分野
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機能
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. -
組織特異性
Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216. -
細胞内局在
Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane. - Information by UniProt
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別名
- Glycogen Synthase Kinase 3 Beta
- Glycogen synthase kinase-3 beta
- GSK 3 beta
see all -
参照データベース
- Entrez Gene: 56637 Mouse
- SwissProt: Q9WV60 Mouse
- Unigene: 394930 Mouse
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab243994の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
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Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Sandwich ELISA - Mouse GSK3 beta Antibody Pair - BSA and Azide free (ab243994)Representative standard curve from corresponding SimpleStep ELISA® Kit (ab218264), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.
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Sandwich ELISA - Mouse GSK3 beta Antibody Pair - BSA and Azide free (ab243994)To learn more about the advantages of recombinant antibodies see here.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab243994 は論文での使用が確認できていません。
