MEF2D 抗体 Pair - BSA and Azide free (ab300178)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Mouse, Rat, Human
製品の概要
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製品名
MEF2D Antibody Pair - BSA and Azide free -
アッセイタイプ
ELISA set -
検出範囲
78 pg/ml - 10000 pg/ml -
種交差性
交差種: Mouse, Rat, Human -
製品の概要
MEF2D Antibody Pair - BSA and Azide free (AB300178) is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify human MEF2D in sandwich ELISAs and many other pair-based applications.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The pair can be used in variety of assays and platforms including but not limited to:
- Sandwich ELISA
- FRET/TR-FRET/HTRF
- Meso Scale Discovery® (MSD®)
- Bead-based assays
- AlphaLISA®/AlphaScreen®
- DELFIA® immunoassays
- Simoa® and Single Molecule Counting (SMC™) immunoassays
- Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
- Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
- Concentration of ~1 mg/ml as measured by the protein A280 method.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration: ~1 mg/ml
Storage buffer: 100% PBS
Form: Liquid
Isotype: IgG
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
For more information see here.
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Reagents
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
キャリア・フリー
はい -
内容 10 x 96 tests ab300179 - MEF2D Capture Antibody (unconjugated) 1 x 100µg ab300180 - MEF2D Detector Antibody (unconjugated) 1 x 100µg -
研究分野
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機能
Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis. -
配列類似性
Belongs to the MEF2 family.
Contains 1 MADS-box domain.
Contains 1 Mef2-type DNA-binding domain. -
発生段階
Present in myotubes and also in undifferentiated myoblasts. -
ドメイン
The beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity. -
翻訳後修飾
Phosphorylated on Ser-444 by CDK5 is required for Lys-439 sumoylation and inhibits transcriptional activity. In neurons, enhanced CDK5 activity induced by neurotoxins promotes caspase 3-mediated cleavage leading to neuron apoptosis. Phsophorylation on Ser-180 can be enhanced by EGF.
Acetylated on Lys-439 by CREBBP. Deacetylated by SIRT1.
Sumoylated on Lys-439 by SUMO2 but not SUMO1; which inhibits transcriptional activity and myogenic activity. Desumoylated by SENP3.
Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation. -
細胞内局在
Nucleus. Translocated by HDAC4 to nuclear dots. - Information by UniProt
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別名
- DKFZp686I1536
- MADS box transcription factor 2 polypeptide D
- Mef2d
see all -
参照データベース
- Entrez Gene: 4209 Human
- Entrez Gene: 17261 Mouse
- Entrez Gene: 81518 Rat
- Omim: 600663 Human
- SwissProt: Q14814 Human
- SwissProt: Q63943 Mouse
- SwissProt: O89038 Rat
- Unigene: 314327 Human
see all
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300178の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Human MEF2D antibody pair
Sandwich ELISA of AB300178 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
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MEF2D Antibody Pair - BSA and Azide free (AB300178)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab300178 は論文での使用が確認できていません。
