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AB312860

Human VEGFA 抗体 Pair - BSA and Azide free

Human VEGFA Antibody Pair - BSA and Azide free

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Human VEGFA Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human VEGFA.

別名を表示する

VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF

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Sandwich ELISA - Human VEGFA Antibody Pair - BSA and Azide free (AB312860)
  • sELISA

Supplier Data

Sandwich ELISA - Human VEGFA Antibody Pair - BSA and Azide free (AB312860)

Sandwich ELISA of ab312860 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

交差種

Human

アッセイタイプ

ELISA set

検出範囲

7.8 - 500 pg/mL

アッセイプラットフォーム

Reagents

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

Human VEGFA Antibody Pair - BSA and Azide free (ab312860) is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human VEGFA in sandwich ELISAs and many other pair-based applications.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The pair can be used in variety of assays and platforms including but not limited to:

  • Sandwich ELISA
  • FRET/TR-FRET/HTRF
  • Meso Scale Discovery® (MSD®)
  • Bead-based assays
  • AlphaLISA®/AlphaScreen®
  • DELFIA® immunoassays
  • Simoa® and Single Molecule Counting (SMC™) immunoassays
  • Multiplex

Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

  • Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
  • Concentration of ~1 mg/ml as measured by the protein A280 method.

We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

Please note:

The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

Antibody properties:

Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

Concentration: ~1 mg/ml

Storage buffer: 100% PBS

Form: Liquid

Isotype: IgG

Recombinant monoclonal antibodies offer several advantages including:

- High batch-to-batch consistency and reproducibility

- Improved sensitivity and specificity

- Long-term security of supply

- Animal-free production

Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

Simoa is a registered trademark of Quanterix, Inc.

SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

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製品内容

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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

VEGF or Vascular Endothelial Growth Factor is a signaling protein that plays an essential role in blood vessel formation. Known also as VEGFA its molecular mass is approximately 45 kDa. VEGF is expressed in a variety of tissues especially those with high angiogenic activity such as endothelial cells macrophages and platelets. This protein activates receptors on the surface of endothelial cells triggering a cascade of intracellular events that lead to cellular proliferation migration and increased vascular permeability.
Biological function summary

This protein regulates angiogenesis the process through which new blood vessels form from pre-existing vasculature. It interacts with specific tyrosine kinase receptors such as VEGFR-1 and VEGFR-2. VEGF is not part of a multi-protein complex but it can form homodimers which significantly enhances its activity. It ensures proper vascularization during development and wound healing by directing the growth and branching of vessels. This makes it an important factor in supplying oxygen and nutrients to tissues.

Pathways

VEGF is primarily involved in the VEGF signaling and PI3K/AKT pathways. These pathways allow it to mediate endothelial cell function and survival. In the VEGF signaling pathway it associates closely with members like VEGFR-2 and PLCγ1 which activate further signal transduction needed for angiogenesis. The PI3K/AKT pathway linked with cell proliferation and survival also interacts with VEGF connecting it to other important proteins like AKT and mTOR highlighting its significant role in cellular responses to environmental stimuli.

VEGF has a strong association with cancer and age-related macular degeneration. In cancer overexpression of VEGF leads to pathological angiogenesis providing tumors with the blood supply needed for growth and metastasis. Tumor cells through hypoxia-inducible factors (HIF) upregulate VEGF expression correlating with cancer progression. In age-related macular degeneration abnormal blood vessel growth influenced by VEGF contributes to visual impairment and blindness further implicating the protein in ocular diseases. Targeted therapies often aim at inhibiting VEGF activity to control the disease progression.
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製品プロトコール

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ターゲットの情報

N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed : 35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity).. VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed : 34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 17446437).. Isoform VEGF165B. Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
See full target information VEGFA
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