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AB218186

Human EGF Matched 抗体 Pair Kit

Human EGF Matched Antibody Pair Kit

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Human EGF Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Human EGF by sandwich ELISA method.

別名を表示する

Pro-epidermal growth factor, EGF

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ELISA - Human EGF Matched Antibody Pair Kit (AB218186)
  • ELISA

Supplier Data

ELISA - Human EGF Matched Antibody Pair Kit (AB218186)

Human EGF standard curve.

Standard calibration curve. Background subtracted values are graphed.

Key facts

検出方法

Colorimetric

交差種

Human

アッセイタイプ

ELISA set

検出感度

= 0.16 pg/mL

検出範囲

0.97 - 62.5 pg/mL

アッセイプラットフォーム

Reagents

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Reactivity data

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製品の詳細

Human EGF Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human EGF. Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification. Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our . An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905). For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab217772. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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製品内容

{ "values": { "5x96Tests": { "sellingSize": "5 x 96 Tests", "publicAssetCode":"ab218186-5x96Tests", "assetComponentDetails": [ { "size":"1 x 12.5 µg", "name":"Human EGF Detector Antibody", "number":"AB218186-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human EGF Lyophilized Protein", "number":"AB218186-CMP03", "productcode":"" }, { "size":"1 x 50 µg", "name":"Human EGF Capture Antibody", "number":"AB218186-CMP01", "productcode":"" } ] } } }
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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
-20°C
長期保存温度
-20°C
保管に関する情報
-20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Epidermal Growth Factor (EGF) also known as urogastrone is a single polypeptide that facilitates cell growth proliferation and differentiation by binding to the Epidermal Growth Factor Receptor (EGFR). The EGF protein is characterized by a relatively small molecular weight of approximately 6.4 kDa. It is expressed in various tissues including the kidney submandibular glands and Brunner's glands in the mouse and other species. The presence of anti-EGF antibodies can further enhance the understanding of its mechanical actions and functions in different organisms.
Biological function summary

The epidermal growth factor plays a pivotal role in the regulation of cell signaling. It functions outside a complex interacting mainly with EGFR located on the cell surface. Upon ligand binding EGF induces the receptor dimerization leading to autophosphorylation which activates intrinsic intracellular signaling cascades. This activation is critical for cellular responses such as DNA synthesis and the progression of the cell cycle. EGF recombinant proteins such as HEGF are utilized in laboratories to examine its biological activity meticulously.

Pathways

EGF engages in key cellular pathways that mediate various essential functions. It significantly activates the MAPK and PI3K-AKT signaling pathways which govern processes such as cell division and survival. These pathways require the participation of several other proteins including Ras Raf and PDK1 which help modulate their downstream effects. The interplay of EGF with associated proteins highlights its importance in maintaining proper signaling dynamics necessary for healthy cellular function. EGF ELISA kits as well as EGF ELISAs are tools developed for robust analysis of its pathway interactions in research and clinical settings.

Altered EGF signaling is associated with increased risk of certain cancers and inflammatory diseases. Overexpression or mutations in the EGF-EGFR axis can lead to the progression of carcinomas such as those found in the lung and breast as well as disorders like psoriasis. These aberrant signaling events are often linked with other proteins such as HER2 and VEGF which can exacerbate disease severity. The study and detection of EGF products therefore offer significant insights into both the progression and potential therapeutic targeting of these conditions.
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製品プロトコール

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ターゲットの情報

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed : 10964941).
See full target information EGF
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websiteProtocolBooklet
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組合せ製品

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Primary Antibodies

AB242990

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RabMAb|Recombinant

Sandwich ELISA - Anti-EGF antibody [EPR19899-46] - BSA and Azide free (Detector) (AB242990)

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