Human c-Myc 抗体 Pair - BSA and Azide free (ab290702)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
製品の概要
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製品名
Human c-Myc Antibody Pair - BSA and Azide free
c-Myc キット 製品一覧 -
アッセイタイプ
ELISA set -
検出範囲
62.5 pg/ml - 8000 pg/ml -
種交差性
交差種: Human -
製品の概要
Human c-Myc Antibody Pair - BSA and Azide free (ab290702) is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human c-myc in sandwich ELISAs and many other pair-based applications.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The pair can be used in variety of assays and platforms including but not limited to:
- Sandwich ELISA
- FRET/TR-FRET/HTRF
- Meso Scale Discovery® (MSD®)
- Bead-based assays
- AlphaLISA®/AlphaScreen®
- DELFIA® immunoassays
- Simoa® and Single Molecule Counting (SMC™) immunoassays
- Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
- Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
- Concentration of ~1 mg/ml as measured by the protein A280 method.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration: ~1 mg/ml
Storage buffer: 100% PBS
Form: Liquid
Isotype: IgG
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
For more information see here.
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Reagents
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
キャリア・フリー
はい -
内容 10 x 96 tests ab290674 - Human c-Myc Capture Antibody (unconjuated) 1 x 100µg ab290675 - Human c-Myc Detector Antibody (unconjuated) 1 x 100µg -
研究分野
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機能
Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes. -
関連疾患
Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
翻訳後修飾
Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. -
細胞内局在
Nucleus > nucleoplasm. Nucleus > nucleolus. - Information by UniProt
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別名
- AU016757
- Avian myelocytomatosis viral oncogene homolog
- bHLHe39
see all -
参照データベース
- Entrez Gene: 4609 Human
- Omim: 190080 Human
- SwissProt: P01106 Human
- Unigene: 202453 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab290702の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Human c-Myc Antibody Pair - BSA and Azide free
Sandwich ELISA of ab290702 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
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Human c-Myc Antibody Pair - BSA and Azide freeInterpolated concentrations of native c-Myc in human control wild type HEK293T cell and MYC knockout HEK293T cell based on 1000 µg/mL extract loads. The concentrations of c-Myc were measured in duplicate and interpolated from the c-Myc standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean c-Myc concentration was determined to be 4509.9 pg/mL in wild type HEK293T extract (Human wild-type HEK293T cell line ab255449) and undetectable in MYC knockout HEK293T extract (Human MYC knockout HEK293T cell line ab256500).
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Human c-Myc Antibody Pair - BSA and Azide free (ab290702)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab290702 は論文での使用が確認できていません。
