Human BST2 抗体 Pair - BSA and Azide free (ab241912)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
製品の概要
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製品名
Human BST2 Antibody Pair - BSA and Azide free
BST2/Tetherin キット 製品一覧 -
アッセイタイプ
ELISA set -
検出範囲
7.8 pg/ml - 500 pg/ml -
種交差性
交差種: Human -
製品の概要
The Antibody Pair can be used to quantify Human BST2. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab231931), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Reagents
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
キャリア・フリー
はい -
内容 10 x 96 tests Human BST2 Capture Antibody (unconjugated) 1 x 100µg Human BST2 Detector Antibody (unconjugated) 1 x 100µg -
研究分野
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機能
May be involved in the sorting of secreted proteins (By similarity). May be involved in pre-B-cell growth. Antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. May play a role in B-cell activation in rheumatoid arthritis. -
組織特異性
Predominantly expressed in liver, lung, heart and placenta. Lower levels in pancreas, kidney, skeletal muscle and brain. Overexpressed in multiple myeloma cells. Highly expressed during B-cell development, from pro-B precursors to plasma cells. Highly expressed on T-cells, monocytes, NK cells and dendritic cells (at protein level). -
配列類似性
Belongs to the tetherin family. -
ドメイン
The extracellular coiled coil domain is important for virus retention at the cell surface and prevention of virus spreading. -
翻訳後修飾
Monoubiquitinated by KSHV E3 ubiquitin-protein ligase K5, leading to its targeting to late endosomes and degradation. -
細胞内局在
Golgi apparatus > trans-Golgi network. Cell membrane. Cell membrane. Late endosome. Targeted to late endosomes upon KSHV infection and subsequent ubiquitination. Targeted to the trans-Golgi network by viral VPU protein. - Information by UniProt
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別名
- Bone marrow stromal antigen 2
- Bone marrow stromal cell antigen
- Bone marrow stromal cell antigen 2
see all -
参照データベース
- Entrez Gene: 684 Human
- Omim: 600534 Human
- SwissProt: Q10589 Human
- Unigene: 118110 Human
関連製品
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Alternative Versions
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab241912の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
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Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Sandwich ELISA - Human BST2 Antibody Pair - BSA and Azide free (ab241912)Representative standard curve from corresponding SimpleStep ELISA® Kit (ab231931), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.
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Sandwich ELISA - Human BST2 Antibody Pair - BSA and Azide free (ab241912)Representative standard curve from corresponding SimpleStep ELISA® Kit (ab231931), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits. -
Sandwich ELISA - Human BST2 Antibody Pair - BSA and Azide free (ab241912)To learn more about the advantages of recombinant antibodies see here.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab241912 は論文での使用が確認できていません。
