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AB316996

Human beta 2 Defensin/BD-2 抗体 Pair - BSA and Azide free

Human beta 2 Defensin/BD-2 Antibody Pair - BSA and Azide free

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Human beta 2 Defensin/BD-2 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human beta 2 Defensin/BD-2.

別名を表示する

DEFB102, DEFB2, DEFB4, DEFB4B, DEFB4A, Defensin beta 4A, Beta-defensin 2, Skin-antimicrobial peptide 1, BD-2, hBD-2, SAP1

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Sandwich ELISA - Human beta 2 Defensin/BD-2 Antibody Pair - BSA and Azide free (AB316996)
  • sELISA

Supplier Data

Sandwich ELISA - Human beta 2 Defensin/BD-2 Antibody Pair - BSA and Azide free (AB316996)

Sandwich ELISA of ab316996 with the capture antibody ab316997 dilution at 2 ug/mL and detector antibody ab316998 dilution at 0.5 ug/mL.

Key facts

交差種

Human

アッセイタイプ

ELISA set

検出範囲

1.56 - 100 pg/mL

アッセイプラットフォーム

Reagents

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組合せ製品

Proteins & Peptides

AB243124

Recombinant human beta 2 Defensin/BD-2 protein (Active)

Bioactive

SDS-PAGE - Recombinant human beta 2 Defensin/BD-2 protein (Active) (AB243124)

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  • 0
Assay Kits

AB314384

Human S100A7 ELISA Kit

Sandwich ELISA - Human S100A7 ELISA Kit (AB314384)

  • 0
  • 0
Primary Antibodies

AB63982

Anti-beta 2 Defensin/BD-2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Defensin/BD-2 antibody (AB63982)

  • 5
  • 1
Primary Antibodies

AB282273

Anti-Psoriasin antibody [EPR23482-210] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Psoriasin antibody [EPR23482-210] - BSA and Azide free (Detector) (AB282273)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The pair can be used in variety of assays and platforms including but not limited to:

  • Sandwich ELISA
  • FRET/TR-FRET/HTRF
  • Meso Scale Discovery® (MSD®)
  • Bead-based assays
  • AlphaLISA®/AlphaScreen®
  • DELFIA® immunoassays
  • Simoa® and Single Molecule Counting (SMC™) immunoassays
  • Multiplex

Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

  • Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
  • Concentration of ~1 mg/ml as measured by the protein A280 method.

We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

Please note:

The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

Antibody properties:

Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

Concentration: ~1 mg/ml

Storage buffer: 100% PBS

Form: Liquid

Isotype: IgG

Recombinant monoclonal antibodies offer several advantages including:

- High batch-to-batch consistency and reproducibility

- Improved sensitivity and specificity

- Long-term security of supply

- Animal-free production

Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

Simoa is a registered trademark of Quanterix, Inc.

SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

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製品内容

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab316996-10x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human beta 2 Defensin/BD-2 Detector Antibody (unconjugated)", "number":"AB316996-CMP02", "productcode":"AB316998" }, { "size":"1 x 100 µg", "name":"Human beta 2 Defensin/BD-2 Capture Antibody (unconjugated)", "number":"AB316996-CMP01", "productcode":"AB316997" } ] } } }
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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Beta 2 defensin also known as BD-2 or beta-defensin 2 is a small antimicrobial peptide with a molecular mass of about 4.6 kDa. It plays an important role in the innate immune response by disrupting microbial membranes. BD-2 is expressed in epithelial cells making it important for creating a protective barrier in the skin respiratory tract and urogenital tract. This peptide acts directly against bacteria fungi and some viruses helping to maintain microbial homeostasis in these areas.
Biological function summary

Beta 2 defensin serves as a critical component of host defense mechanisms. It not only shows antimicrobial properties but also influences inflammatory processes. BD-2 interacts with immune cells recruiting and activating them for enhanced defense response. Presently there is no evidence of beta 2 defensin being part of a larger protein complex focusing its function on immediate immune response and cellular signaling in areas it is expressed.

Pathways

Beta 2 defensin engages in critical pathways of immune protection. It is part of the toll-like receptor signaling pathway and engages the NF-kB pathway. These pathways are essential for activating immune responses when pathogens are recognized. Additionally beta 2 defensin interacts with other defensins and related antimicrobial peptides to mount an effective immune response coordinating with various cytokines and chemokines to balance protective actions.

Beta 2 defensin has connections to inflammatory and autoimmune conditions. It has a potential link to psoriasis where its expression levels are increased serving both protective and inflammatory roles. BD-2 is also investigated in cystic fibrosis where its impaired function can contribute to chronic lung infections. In these conditions the altered balance of BD-2 associates it with proteins involved in immune dysregulation contributing to the pathophysiology of these disorders.
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製品プロトコール

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ターゲットの情報

Exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria, with highest activity against Gram-negative bacteria (PubMed : 10837369, PubMed : 9202117). Antimicrobial activity against P.aruginosa seems to be salt-sensitive and is reduced with high salt concentrations greater than 25 mM (PubMed : 10837369). Also exhibits antimicrobial activity against the yeast C.albicans (PubMed : 10837369, PubMed : 30050988, PubMed : 9202117). Permeabilizes C.albicans cell membranes via targeting plasma membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP2), thereby leading to cell fragmentation and cell death (PubMed : 30050988). Acts as a ligand for C-C chemokine receptor CCR6 (PubMed : 10521347, PubMed : 20068036). Binds to CCR6 and induces chemotactic activity of CCR6-expressing cells, such as immature dendritic cells and memory T cells (PubMed : 10521347, PubMed : 20068036).
See full target information DEFB4A
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