Human Obesity 抗体 Array - Membrane (62 targets) (ab169819)
製品の概要
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製品名
Human Obesity Antibody Array - Membrane (62 targets)
Obesity Antibody Array antibody arrays 製品一覧 -
サンプルの種類
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media -
アッセイタイプ
Semi-quantitative -
種交差性
交差種: Human -
製品の概要
Abcam's Human Obesity Antibody Array - Membrane (62 targets) detects 62 Human Adipokines in 4 or 8 samples. Suitable for all liquid sample types.
Targets:4-1BB, ACE-2, Adiponectin, Adipsin, AgRP, Angiopoietin 1, Angiopoietin 2, ANGPTL4, CRP, ENA-78, Fas, FGF-6, Growth Hormone, HCC-4, IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGF-1, IGF-1 sR, IL-1 R4, IL-1 sRI, IL-10, IL-11, IL-12, IL-1 alpha, IL-1 beta, IL-6, IL-6 sR, IL-8, Insulin, IP-10, Leptin R, Leptin, LIF, Lymphotactin, MCP-1, MCP-3, M-CSF, MIF, MIP-1 beta, MSP-alpha, Osteoprotegerin, OSM , PAI-I, PARC, PDGF-AA, PDGF-AB, PDGF-BB, RANTES, Resistin, Serum Amyloid A , SDF-1 , sTNFRII, sTNFRI, TECK, TGF beta 1, TIMP-1, TIMP-2, TNF alpha, VEGF-A, XEDAR
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特記事項
If you are interested in this cytokine array, arrays ab133998 and ab134000 may also be of interest. A list of human antibody membrane arrays is available here.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
アプリケーション
適用あり: Multiplex Protein Detectionmore details
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 4 Membranes 1 x 8 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1X Blocking Buffer 1 x 25ml 2 x 25ml 2000X Biotin-Conjugated Anti Adipokines 2 vials 4 vials 20X Wash Buffer I 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit Detection Buffer C 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml Obesity Antibody Array Membranes 4 units 8 units -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab169819の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Multiplex Protein Detection |
Use at an assay dependent concentration.
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| 特記事項 |
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Multiplex Protein Detection
Use at an assay dependent concentration. |
画像
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Muliplex Protein Detection - Human Obesity Antibody Array - Membrane (62 targets) (ab169819)This image is courtesy of an anonymous Abreview
5 mg of lyophilized microdissected skeletal muscle (vastus laterals) was homogenized in 180 uL 1X cell lysis buffer (included in kit). After a 10,000 x g 5 min spin at 4°C removed the supernatant was removed and the total protein determined. 250 µg of total protein was added to each membrane with overnight incubations for each incubation step. The attached image is the first blot setting up the method and was exposed using a FluorChem M for 27 seconds. As can be seen Acrp30 will require less protein/less exposure but otherwise all but seven cytokines were visualized following this protocol.
Abreview rating 5/5 stars. Review from Abcam user community. Verified customer at Baltimore VA Medical Center. Submitted Oct 9 2014.
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Multiplex Protein Detection - Human Obesity Antibody Array - Membrane (62 targets) (ab169819)Typical images obtained with Abcam Human Cytokine Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab169819 は 4 報の論文で使用されています。
- Lee M et al. Anti-obesity effect of vegetable juice fermented with lactic acid bacteria isolated from kimchi in C57BL/6J mice and human mesenchymal stem cells. Food Funct 14:1349-1356 (2023). PubMed: 36630124
- Serra MC et al. Resistance training reduces inflammation and fatigue and improves physical function in older breast cancer survivors. Menopause 25:211-216 (2018). PubMed: 28832427
- Mustapic M et al. Plasma Extracellular Vesicles Enriched for Neuronal Origin: A Potential Window into Brain Pathologic Processes. Front Neurosci 11:278 (2017). PubMed: 28588440
- Gernapudi R et al. Targeting exosomes from preadipocytes inhibits preadipocyte to cancer stem cell signaling in early-stage breast cancer. Breast Cancer Res Treat 150:685-95 (2015). PubMed: 25783182